This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.

Key Features

@introbul:Key Features @bul:* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies * Includes overviews of @subbul:* Optical probes and reagents * Fabrication and use of ion-selective microelectrodes * Use of NMR spectroscopy * Ionophores

Table of Contents

Light-Optical-Based Reagents for the Measurement and Manipulation of Ions, Metabolites, and Macromolecules in Living Cells, K.A. Giuliano and D.L. Taylor. Use of Ion-Selective Microelectrodes for Recording Intracellular Ion Levels, M.E. Morris. Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations, S.J. Karlik. Use of Ionophores for Manipulating Intracellular Ion Concentrations, G.A. Woolley, D.R. Pfeiffer, and C.M. Deber. Fluorescence Imaging of Cytosolic Calcium: An Introduction to Basic Experimental Principles, D.A. Williams. Fluorescence Measurements of Cytosolic Calcium: Combined Photometry with Electrophysiology, W.T. Mason, J. Dempster, R. Zorec, J. Joyland, and P.M. Lledo. Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium, A.M. Gurney and S.E. Bates. Measurement of Cytosolic Calcium by 19 NMR, G.A. Smith and H.S. Bachelard. Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes, S.J. Dixon and J.X. Wilson. Brain Cell Acid-Base Changes Measured with Ion-Selective Microelectrodes, C.D. Lascola and R.P. Kraig. Measurement of Cytosolic pH by Nuclear Magnetic Resonance Spectroscopy, O.A.C. Petroff and J.W. Prichard. Manipulation and Regulation of Cytosolic pH, M.O. Bevensee and W.F. Boron. Fluorescence Measurements of Cytosolic Sodium Concentration, M.A. Robertson and J.K. Foskett. Measurement of Cytosolic Sodium Using Ion-Selective Microelectrodes, J.W. Deitmer and T. Munsch. A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic Free Magnesium, E. Murphy and R.E. London. Measurement of Cytosolic Magnesium by 31P NMR Sp


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© 1995
Academic Press
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About the serial-volume-editors

Jacob Kracier

Affiliations and Expertise

The University of Western Ontario, Health Sciences Centre, London, Canada and Human Frontier Science Program, Strasbourg, France

S. Dixon

Affiliations and Expertise

The University of Western Ontario, Health Sciences Centre, London, Canada