Description

Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.

Key Features

  • Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progress
  • Includes detailed protocols that address real-life needs in laboratory practice
  • Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceutics
  • Compiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins

Readership

Analytical chemists, biochemists, clinical chemists, biologists, including graduate-level students in these disciplines; genetic and biomedical researchers

Table of Contents

Acknowledgments

Preface

Biography

Pier Giorgio Righetti

Egisto Boschetti

Abbreviations

Trade Names

Antiphon

Chapter 1. Introducing Low-Abundance Species in Proteome Analysis

1.1 The Proteomic Arena

1.2 Low-Abundance Protein Definition and Their Tracking Interest

1.3 Categories of Low-Abundance Proteins

1.4 The Proposal for Low-Abundance Protein Access

1.5 References

Chapter 2. Chromatographic and Electrophoretic Prefractionation Tools in Proteome Analysis

2.1 Introduction

2.2 Prefractionation Tools in Proteome Analysis

2.3 Sample Prefractionation via Different Electrophoretic Techniques

2.4 Conclusions

2.5 References

Chapter 3. Current Low-Abundance Protein Access

3.1 General Situation

3.2 High-Abundance Protein Subtraction

3.3 Enrichment of Low-Abundance, Post-translationally Modified Protein Groups

3.4 Low-Abundance Protein Grouping by Common Interaction

3.5 Importance and Limits of Classical Affinity-Enrichment Methods

3.6 Integration of Low-Abundance Protein Access Methods

3.7 References

Chapter 4. Low-Abundance Protein Access by Combinatorial Peptide Libraries

4.1 Combinatorial Chemistry

4.2 Combinatorial Peptide Libraries

4.3 Preparation of Solid-phase Combinatorial Peptide Ligand Libraries

4.4 Use of Peptide Ligand Libraries as a Means of Reducing Protein Dynamic Range Concentration

4.5 Peptide Ligand Library Properties in Protein Capturing

4.6 Elution Mechanism Diversity and Complexity

4.7 Selection of Elution Processes as a Function of Analytical Methods

4.8 Towards a two-dimensional protein capture?

4.9 References

Chapter 5. Plant Proteomics and Food and Beverage Analysis via CPLL Capture

5.1 Introducing Global Protein Analysis in Food

5.2

Details

No. of pages:
364
Language:
English
Copyright:
© 2013
Published:
Imprint:
Elsevier
Electronic ISBN:
9780124045965
Print ISBN:
9780124017344

About the authors

Reviews

"The book was written by the inventors of and leading experts in the technology and provides a comprehensive overview of what has been achieved with this relatively new approach. The detailed description of important experimental parameters and the protocols provided in this book make it a unique resource for all practitioners in the field and all scientists interested in the technology."--Analytical & Bioanalytical Chemistry, February 2014
"Boschetti and Righetti introduce the new technique of combinatorial peptide ligand libraries for identifying low-abundance peptide fragments. The standard proteomics techniques of mass spectrometry, 2D electrophoresis and chromatography are deliberately given brief treatment only as prefractionation tools in favor of new methodologies."--Reference & Research Book News, October 2013