Liquid-Liquid Phase Coexistence and Membraneless Organelles

Liquid-Liquid Phase Coexistence and Membraneless Organelles

1st Edition - January 14, 2021
This is the Latest Edition
  • Editor: Christine D. Keating
  • eBook ISBN: 9780128211601
  • Hardcover ISBN: 9780128211595

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Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Using a sequence-specific coarse-grained model for studying protein liquid–liquid phase separation

    Roshan M. Regy, Wenwei Zheng and Jeetain Mittal

    2. Reconstituting bacterial cell division assemblies in crowded, phase-separated media

    Begoña Monterroso, Miguel Ángel Robles-Ramos, Silvia Zorrilla and Germán Rivas

    3. Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform

    Alexander F. Mason, Wiggert J. Altenburg, Shidong Song, Marleen van Stevendaal and Jan C.M. van Hest

    4. Cell-free reconstitution of multi-condensate assemblies

    Andrea Putnam and Geraldine Seydoux

    5. Practical considerations for generation of multi-compartment complex coacervates

    Gregory A. Mountain and Christine D. Keating

    6. Methods for characterizing the material properties of biomolecular condensates

    Ibraheem Alshareedah, Taranpreet Kaur and Priya R. Banerjee

    7. Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit

    Erik W. Martin, Jesse B. Hopkins and Tanja Mittag

    8. SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes

    Amanda B. Marciel, Samanvaya Srivastava, Jeffrey M. Ting and Matthew V. Tirrell

    9. Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates

    Vivek M. Prabhu, Samim Ali, Markus Bleuel, Yimin Mao and Yuanchi Ma

    10. Incorporation of proteins into complex coacervates

    Whitney C. Blocher McTigue and Sarah L. Perry

    11. Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation

    Raghav R. Poudyal, McCauley O. Meyer and Philip C. Bevilacqua

    12. Reversible photocontrol of DNA coacervation

    Suzanne Lafon and Nicolas Martin

    13. Enzymatic control over coacervation

    Karina K. Nakashima, Alain A.M. André and Evan Spruijt

Product details

  • No. of pages: 406
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: January 14, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128211601
  • Hardcover ISBN: 9780128211595

About the Serial Volume Editor

Christine D. Keating

Christine Keating is at Penn State University, PA, USA

Affiliations and Expertise

Penn State University, PA, USA