Light and Video Microscopy

2nd Edition

Authors: Randy Wayne
Hardcover ISBN: 9780124114845
eBook ISBN: 9780124115361
Imprint: Academic Press
Published Date: 2nd January 2014
Page Count: 366
Tax/VAT will be calculated at check-out
Compatible Not compatible
VitalSource PC, Mac, iPhone & iPad Amazon Kindle eReader
ePub & PDF Apple & PC desktop. Mobile devices (Apple & Android) Amazon Kindle eReader
Mobi Amazon Kindle eReader Anything else

Institutional Access


The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

Key Features

  • Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope
  • Clearly develops all ideas from historical and logical foundations
  • Laboratory exercises included to assist the reader with practical applications
  • Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope


Cell biologists, neurobiologists, reproductive biologists, plant biologists, microbiologists, fungal biologists, veterinarians, forensic scientists, food scientists, horticulturalists, biochemists, physicists, engineers, and anyone using a light or video microscope

Table of Contents


Preface to the Second Edition

Preface to the First Edition

Chapter 1. The Relation Between the Object and the Image

Luminous and Nonluminous Objects

Object and Image

Theories of Vision

Light Travels in Straight Lines

Images Formed in a Camera Obscura: Geometric Considerations

Where Does Light Come From?

How Can the Amount of Light Be Measured?

Web Resources

Chapter 2. The Geometric Relationship Between Object and Image

Reflection by a Plane Mirror

Reflection by a Curved Mirror

Reflection from Various Sources

Images Formed by Refraction at a Plane Surface

Images Formed by Refraction at a Curved Surface

Fermat’s Principle

Optical Path Length

Lens Aberrations

Geometric Optics and Biology

Geometric Optics of the Human Eye

Web Resources

Chapter 3. The Dependence of Image Formation on the Nature of Light

Christiaan Huygens and the Invention of the Wave Theory of Light

Thomas Young and the Development of the Wave Theory of Light

James Clerk Maxwell and the Wave Theory of Light

Ernst Abbe and the Relationship of Diffraction to Image Formation

Resolving Power and the Limit of Resolution


Web Resources

Chapter 4. Bright-Field Microscopy

Components of the Microscope

The Optical Paths of the Light Microscope

Using the Bright-Field Microscope

Depth of Field

Out-of-Focus Contrast

Uses of Bright-Field Microscopy

Care and Cleaning of the Light Microscope

Web Resources

Chapter 5. Photomicrography

Setting up the Microscope for Photomicrography

Scientific History of Photography

General Nature of the Photographic Process

The Resolution of the Film

Exposure and Composition

The Simi


No. of pages:
© Academic Press 2014
Academic Press
eBook ISBN:
Hardcover ISBN:
Paperback ISBN:

About the Author

Randy Wayne

Affiliations and Expertise

Department of Plant Biology Cornell University, Ithaca, NY, USA


"As such, this book would be an excellent undergraduate-level text on optical microscopy for biologists. It is also valuable to anyone using a light microscope." --Carol Bayles, Cornell University, Ithaca, NY, USA