The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

Table of Contents


Preface to the Second Edition

Preface to the First Edition

Chapter 1. The Relation Between the Object and the Image

Luminous and Nonluminous Objects

Object and Image

Theories of Vision

Light Travels in Straight Lines

Images Formed in a Camera Obscura: Geometric Considerations

Where Does Light Come From?

How Can the Amount of Light Be Measured?

Web Resources

Chapter 2. The Geometric Relationship Between Object and Image

Reflection by a Plane Mirror

Reflection by a Curved Mirror

Reflection from Various Sources

Images Formed by Refraction at a Plane Surface

Images Formed by Refraction at a Curved Surface

Fermat’s Principle

Optical Path Length

Lens Aberrations

Geometric Optics and Biology

Geometric Optics of the Human Eye

Web Resources

Chapter 3. The Dependence of Image Formation on the Nature of Light

Christiaan Huygens and the Invention of the Wave Theory of Light

Thomas Young and the Development of the Wave Theory of Light

James Clerk Maxwell and the Wave Theory of Light

Ernst Abbe and the Relationship of Diffraction to Image Formation

Resolving Power and the Limit of Resolution


Web Resources

Chapter 4. Bright-Field Microscopy

Components of the Microscope

The Optical Paths of the Light Microscope

Using the Bright-Field Microscope

Depth of Field

Out-of-Focus Contrast

Uses of Bright-Field Microscopy

Care and Cleaning of the Light Microscope

Web Resources

Chapter 5. Photomicrography

Setting up the Microscope for Photomicrography

Scientific History of Photography

General Nature of the Photographic Process

The Resolution of the Film

Exposure and Composition

The Simi


No. of pages:
© 2014
Academic Press
eBook ISBN:
Print ISBN:
Print ISBN:


"As such, this book would be an excellent undergraduate-level text on optical microscopy for biologists. It is also valuable to anyone using a light microscope." --Carol Bayles, Cornell University, Ithaca, NY, USA