Description

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.

Key Features

  • Covers the most important laboratory methods in cell biology
  • Chapters written by experts in their fields

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

Series Editors

Contributors

Series Page

Preface

Chapter 1. Extraction/Characterization of DNA

1 Purpose

2 Theory

3 Equipment (in order of appearance)

4 Materials (in order of appearance)

5 Protocol

References

Chapter 2. Co-Immunoprecipitation

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 3. Real-Time PCR

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Prepare Workspace

6 Protocol

References

Chapter 4. Single-Cell Gel Electrophoresis Combined with Lesion-Specific Enzymes to Measure Oxidative Damage to DNA

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 5. High-Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 6. Two-Dimensional Difference Gel Electrophoresis (2D DIGE)

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 7. Optimization of Cell Cycle Measurement by Time-Lapse Microscopy

1 Purpose

2 Theory

3 Determination of Cell Cycle Length

4 Equipments

5 Materials

6 Protocol

References

Chapter 8. Transfection Methods

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 9. Cell Enrichment from Human Blood through Red Cell Lysis

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 10. Magnetic, Batch Separation

1 Purpose

2 Theory

3 Equipment

4 Materials

Details

No. of pages:
544
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Print ISBN:
9780124059146
Electronic ISBN:
9780124055490