Description

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.

Key Features

    • Covers the most important laboratory methods in cell biology
    • Chapters written by experts in their fields

    Readership

    Researchers and students in cell, molecular and developmental biology.

    Table of Contents

    Series Editors

    Contributors

    Series Page

    Preface

    Chapter 1. Extraction/Characterization of DNA

    1 Purpose

    2 Theory

    3 Equipment (in order of appearance)

    4 Materials (in order of appearance)

    5 Protocol

    References

    Chapter 2. Co-Immunoprecipitation

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    5 Protocol

    References

    Chapter 3. Real-Time PCR

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Prepare Workspace

    6 Protocol

    References

    Chapter 4. Single-Cell Gel Electrophoresis Combined with Lesion-Specific Enzymes to Measure Oxidative Damage to DNA

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 5. High-Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    5 Protocol

    References

    Chapter 6. Two-Dimensional Difference Gel Electrophoresis (2D DIGE)

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    5 Protocol

    References

    Chapter 7. Optimization of Cell Cycle Measurement by Time-Lapse Microscopy

    1 Purpose

    2 Theory

    3 Determination of Cell Cycle Length

    4 Equipments

    5 Materials

    6 Protocol

    References

    Chapter 8. Transfection Methods

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    5 Protocol

    References

    Chapter 9. Cell Enrichment from Human Blood through Red Cell Lysis

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 10. Magnetic, Batch Separation

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    Details

    No. of pages:
    544
    Language:
    English
    Copyright:
    © 2012
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780124059146
    Electronic ISBN:
    9780124055490