Description

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.

Key Features

  • Covers the most important laboratory methods in cell biology
  • Chapters written by experts in their fields

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

Series Editors

Contributors

Dedication

Preface

Chapter 1. Structural Dynamics of Nucleic Acids by Single-Molecule FRET

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol 1—SMFRET in Sealed Sample Chamber

6 Protocol 2—Flow Cell for SMFRET

References

Chapter 2. Visualizing the Location and the Dynamics of Gene Expression in Living Animals through Bioluminescence Imaging

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 3. Quality Control Protocol for Confocal Systems

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 4. Electron Microscopy of Bone

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 5. Immunostaining

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein–Protein Interaction

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 7. Immunohistochemical Detection of Neuropeptides/Transmitters in Mammalian Brain

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 8. Intravital Imaging of Autoreactive T Cells in Living Animals

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 9. Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 10. High-Throughput Methods for the Detection of Protein Ov

Details

No. of pages:
464
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124072398
Electronic ISBN:
9780124078079