Laboratory Methods in Cell Biology: Imaging - 1st Edition - ISBN: 9780124072398, 9780124078079

Laboratory Methods in Cell Biology: Imaging, Volume 113

1st Edition

Serial Volume Editors: P. Michael Conn
eBook ISBN: 9780124078079
Hardcover ISBN: 9780124072398
Imprint: Academic Press
Published Date: 18th January 2013
Page Count: 464
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Table of Contents

Series Editors

Contributors

Dedication

Preface

Chapter 1. Structural Dynamics of Nucleic Acids by Single-Molecule FRET

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol 1—SMFRET in Sealed Sample Chamber

6 Protocol 2—Flow Cell for SMFRET

References

Chapter 2. Visualizing the Location and the Dynamics of Gene Expression in Living Animals through Bioluminescence Imaging

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 3. Quality Control Protocol for Confocal Systems

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 4. Electron Microscopy of Bone

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 5. Immunostaining

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein–Protein Interaction

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 7. Immunohistochemical Detection of Neuropeptides/Transmitters in Mammalian Brain

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 8. Intravital Imaging of Autoreactive T Cells in Living Animals

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 9. Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 10. High-Throughput Methods for the Detection of Protein Overexpression Using Fluorescence Markers

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 11. Correlative Microscopy

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocols

References

Chapter 12. Stereology of the Lung

1 Purpose

2 Theory

3 Equipments and Materials

4 Protocols

References

Chapter 13. Quantitative 3D Cell-Based Assay Performed with Cellular Spheroids and Fluorescence Microscopy

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 14. Optimizing Fluorescence Excitation and Detection for Intravital Two-Photon Microscopy

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 15. Two-Dimensional Crystallization by Dialysis for Structural Studies of Membrane Proteins by the Cryo-EM Method Electron Crystallography

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 16. In situ Hybridization

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 17. RNA Imaging

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Index

Volumes in Series

Color Plate


Description

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.

Key Features

  • Covers the most important laboratory methods in cell biology
  • Chapters written by experts in their fields

Readership

Researchers and students in cell, molecular and developmental biology


Details

No. of pages:
464
Language:
English
Copyright:
© Academic Press 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124078079
Hardcover ISBN:
9780124072398

About the Serial Volume Editors

P. Michael Conn Serial Volume Editor

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA