This book sets out clearly and effectively the preparation and working methods of laboratory techniques involving isolated hepatocytes and will make life easier for every laboratory worker concerned with these techniques.

Table of Contents

Chapter 1. Background. 1.1. The development of the isolated hepatocyte preparation. 1.2. Factors involved in cellular organization and adhesion. 1.3. Mechanical methods for separation of parenchymal liver cells. 1.4. Development of enzymatic methods for isolated hepatocyte preparation. 1.5. Principles of cell separation. 1.6. Separation of cells from solid organs other than liver. 1.7. Advantages of the isolated hepatocyte preparation. 2. High-yield preparation of isolated hepatocytes from rat liver. 2.1 Introduction. 2.2. One-step method. 2.3. Two-step procedure. 2.4. Evaluation of common modifications to the recommended method. 2.5. Initial determination of cell quality. 2.6. Assessment of cell yield. 2.7. Reasons for unsatisfactory preparations. 2.8. Removal of damaged cells from the preparation. 2.9. Storage of hepatocytes. 3. Other methods for hypatocyte preparation. 3.1. Introduction. 3.2. Isolated hepatocyte preparation from liver slices. 3.3. Preparation of intact isolated hepatocytes without collagenase. 3.4. Preparation of isolated hepatocytes from species other than rat. 3.5. Preparation of hepatocytes from foetal or neonatal animals. 3.6. Preparation of hepatocytes from abnormal animals. 3.7. Preparation of suspensions of damaged hepatocytes. 3.8. Preparation and purification of non-parenchymal cells. 4. Assessment of integrity of isolated hepatocytes. 4.1. Introduction. 4.2. Measures of cellular integrity. 4.3. Additional methods for determining cellular integrity. 5. Microscopy of isolated hepatocytes. 5.1. Light microscopy of isolated hepatocytes. 5.2. Morphology of isolated hepatocytes by transmission electron microscopy. 5.3. Techniques. 5.4. Morphological studies yet to be exploited. 5.5. Summary of studies by transmission electron microscopy. 5.6. Scanning electron microscopy. 6. Biochemical properties. 6.1. Basis for expressing cell activity. 6.2. Measurement of cellular composition. 6.3. Cellular composition. 6.4. Experimental conditions. 6


No. of pages:
© 1991
Elsevier Science
eBook ISBN:
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About the editor

About the authors

A.M. Edwards

Affiliations and Expertise

University of South Australia, Department of Medical Biochemistry, The Flinders, Adelaide, Australia


@qu:This book covers almost every aspect of the preparation, culture and use of hepatocytes and does so in great detail and with great excellent book, full of good practical advice... A must for every researcher working with, or thinking of working with, hepatocytes in any context. @source:Hepatocyte Users Group Newsletter @qu:This excellent publication will be of value to those entering the area of hepatocyte preparation for the first time, as well as a source of valuable information to the experienced researcher... @source:FEBS Letters