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Ion Channels: Channel Production and Optical Methods - 1st Edition - ISBN: 9780323853767

Ion Channels: Channel Production and Optical Methods, Volume 653

1st Edition

Serial Volume Editors: Daniel Minor Henry Colecraft
Hardcover ISBN: 9780323853767
Imprint: Academic Press
Published Date: 11th June 2021
Page Count: 412
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Table of Contents

Preface

Daniel L. Minor Jr. and Henry M. Colecraft

Methods to produce and purify ion channels

1. Effective production of oligomeric membrane proteins by EarlyBac

Hiro Furukawa

2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies

Lejla Zubcevic

3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies

Vera Moiseekova-Bell

4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies

Seok-Yong Lee

5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination

William A. Catterall

6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy

Nieng Yan

7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy

Show-Ling Shyng

8. Production of K2P2.1 (TREK-1) for structural studies

Daniel L. Minor Jr.

9. Purification of a native nicotinic receptor

Ryan Hibbs

10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs

Stephen Brohawn

Optical methods for probing and controlling channel function

11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid

Sebastian E. Brauchi

12. Fluorescence lifetime measurement of absolute membrane potential

Evan Miller

13. Fluorescent Toxins as Ion Channel Activity Sensors

Jon Sack

14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer

Manu Ben-Johny

15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers

Thomas Grutter

16. Counting Subunits in Kv Channel Complexes

Rikard Blunck


Description

Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2021
Published:
11th June 2021
Imprint:
Academic Press
Hardcover ISBN:
9780323853767

Ratings and Reviews


About the Serial Volume Editors

Daniel Minor

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA

Affiliations and Expertise

Professor, University of California San Francisco, CA, USA

Henry Colecraft

Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Affiliations and Expertise

Professor of Physiology and Cellular Biophysics and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY, USA