Ion Channels: Channel Production and Optical Methods
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Ion Channels: Channel Production and Optical Methods

1st Edition - June 5, 2021

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  • Editors: Daniel Minor, Henry Colecraft
  • Hardcover ISBN: 9780323853767
  • eBook ISBN: 9780323853774

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Description

Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Preface

    Daniel L. Minor Jr. and Henry M. Colecraft

    Methods to produce and purify ion channels

    1. Effective production of oligomeric membrane proteins by EarlyBac

    Hiro Furukawa

    2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies

    Lejla Zubcevic

    3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies

    Vera Moiseekova-Bell

    4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies

    Seok-Yong Lee

    5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination

    William A. Catterall

    6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy

    Nieng Yan

    7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy

    Show-Ling Shyng

    8. Production of K2P2.1 (TREK-1) for structural studies

    Daniel L. Minor Jr.

    9. Purification of a native nicotinic receptor

    Ryan Hibbs

    10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs

    Stephen Brohawn

    Optical methods for probing and controlling channel function

    11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid

    Sebastian E. Brauchi

    12. Fluorescence lifetime measurement of absolute membrane potential

    Evan Miller

    13. Fluorescent Toxins as Ion Channel Activity Sensors

    Jon Sack

    14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer

    Manu Ben-Johny

    15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers

    Thomas Grutter

    16. Counting Subunits in Kv Channel Complexes

    Rikard Blunck

Product details

  • No. of pages: 420
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: June 5, 2021
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323853767
  • eBook ISBN: 9780323853774

About the Serial Volume Editors

Daniel Minor

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA

Affiliations and Expertise

Professor, University of California San Francisco, CA, USA

Henry Colecraft

Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Affiliations and Expertise

Professor of Physiology and Cellular Biophysics and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY, USA

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