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Daniel L. Minor Jr. and Henry M. Colecraft
Methods to produce and purify ion channels
1. Effective production of oligomeric membrane proteins by EarlyBac
2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies
3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies
4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies
5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination
William A. Catterall
6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy
7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy
8. Production of K2P2.1 (TREK-1) for structural studies
Daniel L. Minor Jr.
9. Purification of a native nicotinic receptor
10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs
Optical methods for probing and controlling channel function
11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid
Sebastian E. Brauchi
12. Fluorescence lifetime measurement of absolute membrane potential
13. Fluorescent Toxins as Ion Channel Activity Sensors
14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer
15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers
16. Counting Subunits in Kv Channel Complexes
Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2021
- 11th June 2021
- Academic Press
- Hardcover ISBN:
Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA
Professor, University of California San Francisco, CA, USA
Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA
Professor of Physiology and Cellular Biophysics and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY, USA
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