Invertebrate Tissue Culture - 1st Edition - ISBN: 9780127099019, 9780323149662

Invertebrate Tissue Culture

1st Edition

Editors: C Vago
eBook ISBN: 9780323149662
Imprint: Academic Press
Published Date: 1st January 1971
Page Count: 456
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Invertebrate Tissue Culture, Volume I, reviews advances in the use of tissue and organ culture in invertebrate research in physiology and pathology. It describes methods in invertebrate tissue culture, including organ culture techniques in liquid and gel media; aseptic rearing of invertebrates for tissue culture; and cell culture of organisms, ranging from Lepidoptera and Diptera to Coleoptera, Orthoptera, Dictyoptera, Hymenoptera, Hemiptera, Crustacea, Arachnida, Merostomacea, and mollusks.
Organized into two sections encompassing 13 chapters, this volume begins with an overview of the general methodology in cell and organ cultures and their preparation from aseptic conditions. It then discusses methods for the examination of cultures, including those concerning ultrastructure studies by electron microscopy. The reader is also introduced to cell cultures obtained from different groups of invertebrates, with emphasis on peculiarities specific to each group; the morphology and physiology of cultured invertebrate cells; and cultivation and growth of cells. This book is a valuable resource for specialists in the field of invertebrate cell and organ culture.

Table of Contents

List of Contributors


Contents of Volume II

I. Methods in Invertebrate Tissue Culture

1 Cell Culture Media and Methods

I. Design of Cell Culture Media

II. Selection of Tissue for Culture

III. Preparation of Tissue for Culture

IV. Cultures in Fluid Medium

V. Vapor Phase

VI. Incubation Temperature


2 Organ Culture Methods

I. Introduction

II. Organ Culture Technique in Liquid Medium

III. Organ Culture Methods on a Gel Medium

IV. Conclusions


3 Electron Microscopy of Cell and Organ Cultures of Invertebrates

I. Introduction

II. General Techniques and the Adaptation of These Techniques to Invertebrate Tissue Culture

III. Results

IV. Conclusion


4 Aseptic Rearing of Invertebrates for Tissue Culture

I. Introduction

II. Sterile Methods for Rearing Invertebrates

III. Conclusions


II. Cell Cultures

5 The Morphology and Physiology of Cultured Invertebrate Cells

I. Introduction

II. Sources and Types of Cells Used

III. Morphology of Cells in Primary Cultures

IV. Cell Lines

V. Growth of Cells in Culture

VI. Metabolism of Cultured Cells


6 Cell Culture of Lepidoptera

I. Introduction

II. Materials and Techniques

III. Cultivation and Growth of Cells


7 Cell Culture of Diptera

I. Introduction

II. Cultures of Drosophila, Calliphora, and Musca Tissues

III. Cultures of Aedes, Culex, Glossina, and Anopheles Tissues

IV. Conclusion


8 Cell Culture of Coleoptera, Orthoptera and Dictyoptera

I. Introduction

II. Cell Culture of Coleoptera

III. Cell Culture of Orthoptera

IV. Cell Culture of Dictyoptera


9 Cell Culture of Hymenoptera

I. Introduction

II. Culture Media

III. Methods

IV. Cultures Obtained


10 Cell Culture of Hemiptera

I. Introduction

II. Leafhopper (Cicadellidae) Cell Culture

III. Aphid (Aphididae) and Planthopper (Delphacidae) Cell Culture


11 Cell Cultures of Crustacea, Arachnida, and Merostomacea

I. Introduction

II. Cell Cultures of Crustacea

III. Cell Cultures of Arachnida

IV. Cell Cultures of Merostomacea


12 Cell Culture of Mollusks

I. Introduction

II. Techniques for Cell Culture of Mollusks

III. Cell Cultures Obtained


13 Cell Culture of Invertebrates Other Than Mollusks and Arthropods

I. Introduction

II. Development of Culture Media

III. Cell Culture of Porifera

IV. Cell Culture of Cnidaria

V. Cell Culture of Platyhelminthes

VI. Cell Culture of Annelids

VII. Cell Culture of Sipunculids

VIII. Cell Culture of Echinoderms

IX. Cell Culture of Stomochordates

X. Cell Culture of Protochordates


Author Index

Subject Index


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© Academic Press 1971
Academic Press
eBook ISBN:

About the Editor

C Vago

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