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Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry - 1st Edition - ISBN: 9780128189986, 9780128189993

Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

1st Edition

Author: Bindesh Shrestha
Paperback ISBN: 9780128189986
eBook ISBN: 9780128189993
Imprint: Elsevier
Published Date: 23rd April 2021
Page Count: 270
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Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules.

The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions.

Key Features

  • Provides the introductory foundations of imaging mass spectrometry for those new to the technique
  • Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work
  • Includes case studies summarizing the imaging MS techniques developed for the class of molecules


Scientists interested in imaging mass spectrometry, including bioanalytical chemists in academia and pharma, many with experience with mass spectrometry (either as mass spec lab scientists/PIs or customers of such mass spec services). Biochemists, biologists, biomedical scientists or other scientists who want to learn more about visualizing biomolecules on tissue

Table of Contents

1. Fundamentals of Imaging Mass Spectrometry
2. Ionization Source for Imaging Mass Spectrometry
3. Sample Preparation and MALDI Matrix Applications
4. Tissue Sectioning for Imaging Mass Spectrometry
5. Matrix for Matrix-assisted Laser Desorption/Ionization (MALDI)
6. Molecule Identification Approaches in Imaging Mass Spectrometry
7. Quantitation Workflow in Imaging Mass Spectrometry
8. Spatial Resolution of Imaging Mass Spectrometry
9. Visualization of Imaging Mass Spectrometry
10. Analysis of imaging mass spectrometry data
11. Multimodal imaging mass spectrometry
12. Validation and Standardization of Imaging Mass Spectrometry
13. Towards Clinical Imaging Mass Spectrometry
14. Case study: Imaging N-Glycan on tissue
15. Case study: Reactive matrix and neurotransmitters and brain metabolites
16. Case study: Endogenous metabolites in mammalian tissues
17. Case study: Imaging of small drug molecules
18. Case study: Imaging Gangliosides


No. of pages:
© Elsevier 2021
23rd April 2021
Paperback ISBN:
eBook ISBN:

About the Author

Bindesh Shrestha

Dr. Bindesh Shrestha is a senior scientist at the Waters Corporation focusing on imaging mass spectrometry. He has co-authored more than two dozen peer-reviewed articles in the field of mass spectrometry spanning over the last decade. His publications have been featured multiple times on the covers of leading journals, including Angewandte Chemie, Analytical Chemistry, Analyst, and more.

Affiliations and Expertise

Senior Application Support Scientist, Waters Corporation, Beverly, MA, USA

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