Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

1st Edition - April 23, 2021

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  • Author: Bindesh Shrestha
  • Paperback ISBN: 9780128189986
  • eBook ISBN: 9780128189993

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Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions.

Key Features

  • Provides the introductory foundations of imaging mass spectrometry for those new to the technique
  • Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work
  • Includes case studies summarizing the imaging MS techniques developed for the class of molecules


Scientists interested in imaging mass spectrometry, including bioanalytical chemists in academia and pharma, many with experience with mass spectrometry (either as mass spec lab scientists/PIs or customers of such mass spec services). Biochemists, biologists, biomedical scientists or other scientists who want to learn more about visualizing biomolecules on tissue

Table of Contents

  • 1. Fundamentals of Imaging Mass Spectrometry
    2. Ionization Source for Imaging Mass Spectrometry
    3. Sample Preparation and MALDI Matrix Applications
    4. Tissue Sectioning for Imaging Mass Spectrometry
    5. Matrix for Matrix-assisted Laser Desorption/Ionization (MALDI)
    6. Molecule Identification Approaches in Imaging Mass Spectrometry
    7. Quantitation Workflow in Imaging Mass Spectrometry
    8. Spatial Resolution of Imaging Mass Spectrometry
    9. Visualization of Imaging Mass Spectrometry
    10. Analysis of imaging mass spectrometry data
    11. Multimodal imaging mass spectrometry
    12. Validation and Standardization of Imaging Mass Spectrometry
    13. Towards Clinical Imaging Mass Spectrometry
    14. Case study: Imaging N-Glycan on tissue
    15. Case study: Reactive matrix and neurotransmitters and brain metabolites
    16. Case study: Endogenous metabolites in mammalian tissues
    17. Case study: Imaging of small drug molecules
    18. Case study: Imaging Gangliosides

Product details

  • No. of pages: 270
  • Language: English
  • Copyright: © Elsevier 2021
  • Published: April 23, 2021
  • Imprint: Elsevier
  • Paperback ISBN: 9780128189986
  • eBook ISBN: 9780128189993

About the Author

Bindesh Shrestha

Dr. Bindesh Shrestha is a senior scientist at the Waters Corporation focusing on imaging mass spectrometry. He has co-authored more than two dozen peer-reviewed articles in the field of mass spectrometry spanning over the last decade. His publications have been featured multiple times on the covers of leading journals, including Angewandte Chemie, Analytical Chemistry, Analyst, and more.

Affiliations and Expertise

Senior Application Support Scientist, Waters Corporation, Beverly, MA, USA

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  • Mike A. Thu Jul 22 2021

    Excellent overview of mass spec imaging

    This book gives an excellent overview of mass spec imaging. It has good information on mass spec imaging from ion sources to sample preparation to molecule quantification/identification strategies. As someone who is familiar with mass spec, but not with imaging, it was very helpful to find a comprehensive guide to the mass spec imaging field. One note, the book primarily focuses on small molecule imaging, perhaps reflecting the current trends. In future editions, it will be helpful to have more material on new technologies such as metal-tag ICP imaging for single cells.