Introduction to Enzymology

Introduction to Enzymology focuses on the processes, methodologies, reactions, and approaches involved in enzyme chemistry. The book first offers information on the hydrolysis of peptides and proteins and fermentation and oxidation of major metabolic fuels. Discussions focus on oxidation of fatty acids, alternative pathways of carbohydrate metabolism, Krebs citric acid cycle, free energy and the concept of bond energy, pyruvate oxidation and acetyl coenzyme A formation, and glycolysis. The text then elaborates on the transfer of oxygen, hydrogen, and electrons and sugars and sugar derivatives. The publication takes a look at polynucleotides and their components, amino acids, and acids and acid derivatives. Topics include carbonic anhydrase, mechanism of action of pyridoxal phosphate enzymes, aromatic ring biosynthesis and metabolism of phenylalanine and tyrosine, metabolism of sulfur-containing amino acids, and oxidation of amino acids. The book is a valuable reference for chemists and researchers interested in enzymology.

Preface

I. Introduction

II. Hydrolysis of Peptides and Proteins

III. Fermentation and Oxidation of Major Metabolic Fuels

Glycolysis

Pyruvate Oxidation and Acetyl Coenzyme A Formation

Free Energy and the Concept of Bond Energy

Reactions of Coenzyme A

The Krebs Citric Acid Cycle

Alternative Pathways of Carbohydrate Metabolism

Oxidation of Fatty Acids

IV. Biological Oxidation: Tranfer of Oxygen, Hydrogen, and Electrons

Pyridine Nucleotides

Oxidation-Reduction Potentials

Flavoproteins

Cytochromes

The Hydroperoxidases: Catalase and Peroxidase

Oxidases and Oxygenases

V. Sugars and Sugar Derivatives

Polysaccharides

Reactions of Uridine Nucleotides

VI. Polynucleotides and their Components

Nucleic Acids

Purines and Pyrimidines

VII. Amino Acids

Amino Acid Decarboxylases

Transamination

Oxidation of Amino Acids

Glutamic Acid and Amino Acids Derived from It

Aspartic Acid, Threonine, and Lysine

The Urea Cycle

β-Hydroxy-α-amino Acids

Metabolism of Sulfur-Containing Amino Acids

Histidine

Aromatic Ring Biosynthesis and Metabolism of Phenylalanine and Tyrosine

Tryptophan

Mechanism of Action of Pyridoxal Phosphate Enzymes

Metabolism of Amines

VIII. Acids and Acid Derivatives

Esters

Carbonic Anhydrase

IX. Organization of Structure and Function

Integration of Biochemical Reactions

Adaptive Enzymes and the Synthesis of Proteins

Appendix: Abbreviations

Subject Index