Introduction to Electron Microscopy for Biologists

Introduction to Electron Microscopy for Biologists

1st Edition - July 2, 2008
  • Editor: Terry Allen
  • Hardcover ISBN: 9780123743206
  • eBook ISBN: 9780080888163

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Description

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).

Key Features


*Basic techniques in transmission and scanning electron microscopy
*Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton
*Discussion on electron microscopy of viruses and virus-cell interactions

Readership

Researchers in all fields of biology who use electron microscopy in their lab and studies.

Table of Contents

  • PART ONE. Exploring the Organisation of the Cell by Electron Microscopy

    Basic Transmission and Scanning Electron Microscopy

    1. Scanning Electron Microscopy at the sub-cellular level-Terry Allen

    2 Electron Probe X-ray microanalysis for the study of cell physiology.
    E. Fernandez-Segura and A.Warley

    3 Preparation of cells and tissues for Immuno EM
    Paul Webster, Heinz Schwarz and Gareth Griffiths

    4 Fixation, Freezing and Embedding for Genetically Altered Tissue
    Heinz Schwarz

    5 Combined video fluorescence and 3-D electron microscopy
    Alexander A. Mironov, Roman S. Polishchuk and Galina V. Beznoussenko

    6. From Live-cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label
    Sheona P Drummond and Terry D Allen

    7 Immunolabelling for High Resolution Scanning Electron Microscopy .- Martin Goldberg

    8 Immunogoldlabeling of Thawed Cryosections
    Peter J. Peters and Jason Pierson

    9. Close-to-Native Ultrastructural Preservation by High Pressure Freezing
    Dimitri Vanhecke, Werner Graber, and Daniel Studer

    10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
    Siegfried Reipert and Gerhard Wiche

    11 Freeze-fracture Cytochemistry in Cell Biology
    Nicholas J. Severs and Horst Robenek

    PART TWO. Electron Microscopy of Specific Cellular Structure
    The Cell Membrane

    12 The Plasma membrane cytoskeleton Akihiro Kusumi

    The Cytoskeleton

    13. Correlated Light and Electron Microscopy of the Cytoskeleton
    Sonja Auinger and J.Victor Small

    14 Electron microscopy of intermediate filaments: Teaming up with atomic force and confocal laser scanning microscopy
    Laurent Kreplak, Karsten Richter, Ueli Aebi and Harald Herrmann

    15. Studying Microtubules by Electron Microscopy
    Carolyn Moores

    Cytoplasm, Extracellular Matrix and Cell Junctions

    16. Endoplasmic reticulum and Golgi Complex.
    John Lucoqc

    17. Visualization of Dynamins
    Jason A. Mears and Jenny E. Hinshaw

    18. Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction
    Tobias Starborg, Yinhui Lu, Karl E. Kadler and David F. Holmes

    19 Visualisation of Desmosomes in the Electron Microscope
    Anthea Scothern & David Garrod

    The Nucleus

    20 A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM)
    Stephen Murray and Elena Kiseleva

    21. High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM
    Stephen Murray

    22. Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans
    Merav Cohen, Rachel Santarella, Naama Wiesel, Iain Mattaj, and Yosef Gruenbaum

    23. Visualization of Nuclear Organisation by Ultrastructural Cytochemistry
    Marco Biggiogera and Stanislav Fakan

    24. Scanning Electron Microscopy of Chromosomes
    Gerhard Wanner and Elizabeth Schroeder-Reiter

    PART THREE Cells and Infectious Agents

    25. Infection at the Cellular Level
    Christian Goosmann, Ulrike Abu Abed, and Volker Brinkmann

    26. Electron Microscopy of Viruses and Virus-Cell Interactions
    Peter Wild

Product details

  • No. of pages: 560
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: July 2, 2008
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123743206
  • eBook ISBN: 9780080888163

About the Serial Volume Editor

Terry Allen

Affiliations and Expertise

Paterson Institute, University of Manchester, UK