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Description
This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).
Key Features
*Basic techniques in transmission and scanning electron microscopy
*Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton
*Discussion on electron microscopy of viruses and virus-cell interactions
Readership
Researchers in all fields of biology who use electron microscopy in their lab and studies.
Table of Contents
PART ONE. Exploring the Organisation of the Cell by Electron Microscopy
Basic Transmission and Scanning Electron Microscopy
1. Scanning Electron Microscopy at the sub-cellular level-Terry Allen
2 Electron Probe X-ray microanalysis for the study of cell physiology.
E. Fernandez-Segura and A.Warley
3 Preparation of cells and tissues for Immuno EM
Paul Webster, Heinz Schwarz and Gareth Griffiths
4 Fixation, Freezing and Embedding for Genetically Altered Tissue
Heinz Schwarz
5 Combined video fluorescence and 3-D electron microscopy
Alexander A. Mironov, Roman S. Polishchuk and Galina V. Beznoussenko
6. From Live-cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label
Sheona P Drummond and Terry D Allen
7 Immunolabelling for High Resolution Scanning Electron Microscopy .- Martin Goldberg
8 Immunogoldlabeling of Thawed Cryosections
Peter J. Peters and Jason Pierson
9. Close-to-Native Ultrastructural Preservation by High Pressure Freezing
Dimitri Vanhecke, Werner Graber, and Daniel Studer
10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
Siegfried Reipert and Gerhard Wiche
11 Freeze-fracture Cytochemistry in Cell Biology
Nicholas J. Severs and Horst Robenek
PART TWO. Electron Microscopy of Specific Cellular Structure
The Cell Membrane
12 The Plasma membrane cytoskeleton Akihiro Kusumi
The Cytoskeleton
Details
- No. of pages:
- 560
- Language:
- English
- Copyright:
- © 2008
- Published:
- 2nd July 2008
- Imprint:
- Academic Press
- eBook ISBN:
- 9780080888163
- Print ISBN:
- 9780123743206
About the serial-volume-editor
Terry Allen
Affiliations and Expertise
Paterson Institute, University of Manchester, UK