Introduction to Electron Microscopy for Biologists
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This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).
Key Features
*Basic techniques in transmission and scanning electron microscopy
*Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton
*Discussion on electron microscopy of viruses and virus-cell interactions
Readership
Researchers in all fields of biology who use electron microscopy in their lab and studies.
Table of Contents
- PART ONE. Exploring the Organisation of the Cell by Electron Microscopy
Basic Transmission and Scanning Electron Microscopy
1. Scanning Electron Microscopy at the sub-cellular level-Terry Allen
2 Electron Probe X-ray microanalysis for the study of cell physiology.
E. Fernandez-Segura and A.Warley
3 Preparation of cells and tissues for Immuno EM
Paul Webster, Heinz Schwarz and Gareth Griffiths
4 Fixation, Freezing and Embedding for Genetically Altered Tissue
Heinz Schwarz
5 Combined video fluorescence and 3-D electron microscopy
Alexander A. Mironov, Roman S. Polishchuk and Galina V. Beznoussenko
6. From Live-cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label
Sheona P Drummond and Terry D Allen
7 Immunolabelling for High Resolution Scanning Electron Microscopy .- Martin Goldberg
8 Immunogoldlabeling of Thawed Cryosections
Peter J. Peters and Jason Pierson
9. Close-to-Native Ultrastructural Preservation by High Pressure Freezing
Dimitri Vanhecke, Werner Graber, and Daniel Studer
10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
Siegfried Reipert and Gerhard Wiche
11 Freeze-fracture Cytochemistry in Cell Biology
Nicholas J. Severs and Horst Robenek
PART TWO. Electron Microscopy of Specific Cellular Structure
The Cell Membrane
12 The Plasma membrane cytoskeleton Akihiro Kusumi
The Cytoskeleton
13. Correlated Light and Electron Microscopy of the Cytoskeleton
Sonja Auinger and J.Victor Small
14 Electron microscopy of intermediate filaments: Teaming up with atomic force and confocal laser scanning microscopy
Laurent Kreplak, Karsten Richter, Ueli Aebi and Harald Herrmann
15. Studying Microtubules by Electron Microscopy
Carolyn Moores
Cytoplasm, Extracellular Matrix and Cell Junctions
16. Endoplasmic reticulum and Golgi Complex.
John Lucoqc
17. Visualization of Dynamins
Jason A. Mears and Jenny E. Hinshaw
18. Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction
Tobias Starborg, Yinhui Lu, Karl E. Kadler and David F. Holmes
19 Visualisation of Desmosomes in the Electron Microscope
Anthea Scothern & David Garrod
The Nucleus
20 A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM)
Stephen Murray and Elena Kiseleva
21. High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM
Stephen Murray
22. Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans
Merav Cohen, Rachel Santarella, Naama Wiesel, Iain Mattaj, and Yosef Gruenbaum
23. Visualization of Nuclear Organisation by Ultrastructural Cytochemistry
Marco Biggiogera and Stanislav Fakan
24. Scanning Electron Microscopy of Chromosomes
Gerhard Wanner and Elizabeth Schroeder-Reiter
PART THREE Cells and Infectious Agents
25. Infection at the Cellular Level
Christian Goosmann, Ulrike Abu Abed, and Volker Brinkmann
26. Electron Microscopy of Viruses and Virus-Cell Interactions
Peter Wild
Product details
- No. of pages: 560
- Language: English
- Copyright: © Academic Press 2008
- Published: July 2, 2008
- Imprint: Academic Press
- Hardcover ISBN: 9780123743206
- eBook ISBN: 9780080888163
About the Serial Volume Editor
Terry Allen
Affiliations and Expertise
Paterson Institute, University of Manchester, UK