This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).

Key Features

*Basic techniques in transmission and scanning electron microscopy *Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton *Discussion on electron microscopy of viruses and virus-cell interactions


Researchers in all fields of biology who use electron microscopy in their lab and studies.

Table of Contents

PART ONE. Exploring the Organisation of the Cell by Electron Microscopy Basic Transmission and Scanning Electron Microscopy 1. Scanning Electron Microscopy at the sub-cellular level-Terry Allen 2 Electron Probe X-ray microanalysis for the study of cell physiology. E. Fernandez-Segura and A.Warley 3 Preparation of cells and tissues for Immuno EM Paul Webster, Heinz Schwarz and Gareth Griffiths 4 Fixation, Freezing and Embedding for Genetically Altered Tissue Heinz Schwarz 5 Combined video fluorescence and 3-D electron microscopy Alexander A. Mironov, Roman S. Polishchuk and Galina V. Beznoussenko 6. From Live-cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label Sheona P Drummond and Terry D Allen 7 Immunolabelling for High Resolution Scanning Electron Microscopy .- Martin Goldberg 8 Immunogoldlabeling of Thawed Cryosections Peter J. Peters and Jason Pierson 9. Close-to-Native Ultrastructural Preservation by High Pressure Freezing Dimitri Vanhecke, Werner Graber, and Daniel Studer 10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips Siegfried Reipert and Gerhard Wiche 11 Freeze-fracture Cytochemistry in Cell Biology Nicholas J. Severs and Horst Robenek PART TWO. Electron Microscopy of Specific Cellular Structure The Cell Membrane 12 The Plasma membrane cytoskeleton Akihiro Kusumi The Cytoskeleton


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© 2008
Academic Press
eBook ISBN:
Print ISBN:

About the serial-volume-editor

Terry Allen

Affiliations and Expertise

Paterson Institute, University of Manchester, UK