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Integrated Methods in Protein Biochemistry: Part B
- 1st Edition, Volume 679 - January 18, 2023
- Editor: Arun K. Shukla
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 2 6 4 - 0
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 9 2 6 5 - 7
Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting inter… Read more
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Request a sales quoteIntegrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology series
- Includes the latest information on Integrated Methods in Protein Biochemistry
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
1. Precise modification of native proteins, purification, and analysis of bioconjugates
Vishal Rai
2. NanoBiT-based methods to monitor the activation and modulation of RTKs
Andy Chevigne
3. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling
Ajith Karunarathne
4. Structure and function of bacterial secretion system
Florian Schubot
5. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase
Renwick Dobson
6. Tools and protocols for probing protein sumoylation
Ram Kumar Mishra
7. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase
Thomas C. Brunold and Joshua Miller
8. DeGlyPHER: MS-based analysis of viral spike N-glycoforms
John Yates
9. Covalent protein painting: MS-based protein footprinting
John Yates
10. Characterization of GPCR signaling complexes using negative-staining electron microscopy
Arun K. Shukla
11. Probing protein misfolding and dissociation with free electron laser
Kazuhiro Nakamura and Hisashi Okumura
12. Optimized protocol for the characterization of Cas12a activities
Rakhi Rajan
13. Proximity proteomics for the identification and characterization of extracellular vesicles
Norihiro Kotani
14. Structural and Functional characterization of lytic polysaccharide monooxygenases
Vincent Eijsink
15. Characterization of RRE domain in RiPP biosynthesis
Douglas A. Mitchell
16. Structural and mechanistic insights into ferrous iron import and export systems
Aaron T. Smith
17. Testing anti-cancer drugs with holographic Incoherent light source quantitative phase imaging
Daniel Zicha
18. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry
Nobuaki Takemori
19. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins
Shawn Chen
20. Integrated studies on ageing-related proteins extending life span in yeast
Hiroyui Osada and Makoto Muroi
21. An optimized pipeline for global selenoproteome analysis
Chu Wang
22. High-throughput screening and evolution of carbohydrate-active enzymes
Shishir PS Chundawat
23. Regulation of enzymes with light using unnatural amino acids
Andrea Kneuttinger
24. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies
Sven Eyckerman
25. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics
Emily E. Weinert
26. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors
Nicholas Alexander Graham
27. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules
Joanna F. McGouran
28. Reconstitution of the MLL1 complex and probing methyltransferase activity
Yali Dou
29. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy
Geert van den Bogaart
30. Structural and functional analysis of mono-copper enzymes from fungal species
Vincent Eijsink
Vishal Rai
2. NanoBiT-based methods to monitor the activation and modulation of RTKs
Andy Chevigne
3. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling
Ajith Karunarathne
4. Structure and function of bacterial secretion system
Florian Schubot
5. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase
Renwick Dobson
6. Tools and protocols for probing protein sumoylation
Ram Kumar Mishra
7. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase
Thomas C. Brunold and Joshua Miller
8. DeGlyPHER: MS-based analysis of viral spike N-glycoforms
John Yates
9. Covalent protein painting: MS-based protein footprinting
John Yates
10. Characterization of GPCR signaling complexes using negative-staining electron microscopy
Arun K. Shukla
11. Probing protein misfolding and dissociation with free electron laser
Kazuhiro Nakamura and Hisashi Okumura
12. Optimized protocol for the characterization of Cas12a activities
Rakhi Rajan
13. Proximity proteomics for the identification and characterization of extracellular vesicles
Norihiro Kotani
14. Structural and Functional characterization of lytic polysaccharide monooxygenases
Vincent Eijsink
15. Characterization of RRE domain in RiPP biosynthesis
Douglas A. Mitchell
16. Structural and mechanistic insights into ferrous iron import and export systems
Aaron T. Smith
17. Testing anti-cancer drugs with holographic Incoherent light source quantitative phase imaging
Daniel Zicha
18. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry
Nobuaki Takemori
19. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins
Shawn Chen
20. Integrated studies on ageing-related proteins extending life span in yeast
Hiroyui Osada and Makoto Muroi
21. An optimized pipeline for global selenoproteome analysis
Chu Wang
22. High-throughput screening and evolution of carbohydrate-active enzymes
Shishir PS Chundawat
23. Regulation of enzymes with light using unnatural amino acids
Andrea Kneuttinger
24. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies
Sven Eyckerman
25. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics
Emily E. Weinert
26. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors
Nicholas Alexander Graham
27. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules
Joanna F. McGouran
28. Reconstitution of the MLL1 complex and probing methyltransferase activity
Yali Dou
29. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy
Geert van den Bogaart
30. Structural and functional analysis of mono-copper enzymes from fungal species
Vincent Eijsink
- No. of pages: 420
- Language: English
- Edition: 1
- Volume: 679
- Published: January 18, 2023
- Imprint: Academic Press
- Hardback ISBN: 9780323992640
- eBook ISBN: 9780323992657
AS
Arun K. Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, IndiaRead Integrated Methods in Protein Biochemistry: Part B on ScienceDirect