Immunosensing for Detection of Protein Biomarkers

1st Edition - May 18, 2017
Authors: Huangxian Ju, Guosong Lai, Feng Yan
Language: English
Paperback ISBN:
9 7 8 - 0 - 0 8 - 1 0 1 9 9 9 - 3
eBook ISBN:
9 7 8 - 0 - 0 8 - 1 0 2 0 0 0 - 5

Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achieveme… Read more

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Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achievements in areas such as electrochemical immunosensors, nanoprobe-based immunoassay, chemiluminescence immunoassay, electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging for immunoassay, signal amplification for immunoassay, and so on.

In recent years, immunosensing and immunoassay methods have attracted considerable interest due to their applications in different fields, particularly clinical diagnosis. Although a large number of academic papers in immunosensing and immunoassay have been published in different journals recently, it is still a difficult and time-consuming task for researchers, especially those new to the area, to understand the principles, methods, and research progress of immunosensing.

Based on the research experience of the authors and their research groups, this book offers readers with new research ideas to develop immunosensing methodology. As a monograph, it offers deeper and more complete coverage than review papers, which only report certain aspects of progress. Grounded in the research experience of Professor Ju’s research group, the book focuses on immunosensing for detection of protein biomarkers, summarizing understanding, research, and practice on immunosensing methodology in detection of protein biomarkers.

Chapter 1. Introduction

1.1 Immunoassay

1.2 Immunosensing

1.3 Signal Labels Used in Immunoassay

1.4 Immobilization Method of Immunoreagents

1.5 Perspective

Chapter 2. Signal Amplification for Immunosensing

2.1 Multi-labelling for Signal Amplification

2.2 Nanoparticles as Signal Labels for Signal Amplification

2.3 Enzyme Mimics for Signal Amplification

2.4 PCR for Signal-Amplified Immunosensing

2.5 Rolling Circle Amplification for Amplified Immunoassay

2.6 Hybridization Chain Reaction for Amplified Immunoassay

2.7 Perspective

Chapter 3. Electrochemical Immunosensing

3.1 Introduction

3.2 Direct Immunoreaction Based immunosensors

3.3 Competitive Immunoreaction Based immunosensors

3.4 Sandwich-Type Immunosensors

3.5 Direct Electrochemistry of Enzyme for Immunosensing

3.6 Perspective

Chapter 4. Functional Nanoprobes for Immunosensing

4.1 Nanoprobe as Signal Labels

4.2 Enzyme-Functionalized Nanoprobes

4.3 Noble-Metal Nanoparticles-Functionalized Nanoprobes

4.4 Quantum Dots-Functionalized Nanoprobes

4.5 Other Signal Label-Functionalized Nanoprobes

Chapter 5. Chemiluminescence Immunoassay

5.1 Magnetic Bead Chemiluminescence Immunoassay

5.2 Flow Injection Chemiluminescence Immunoassay

5.3 Capillary Electrophoresis for Chemiluminescence Immunoassay

5.4 Perspective

Chapter 6. Electrochemiluminescent Immunosensing

6.1 Electrochemiluminescence and Electrochemiluminescent Emitters

6.2 Label-Free Electrochemiluminescent Immunoassay

6.3 "Signal-off" Electrochemiluminescent Immunoassay

6.4 "Signal-on" Electrochemiluminescent Immunoassay

6.5 Perspective

Chapter 7. Multianalyte Immunoassay

7.1 Electrode Array Based Multianalyte Immunoassay

7.2 Resolution Strategy for Multianalyte Chemiluminescent Immunoassay

7.3 Chemiluminescence Imaging Multianalyte Immunoassay

7.4 Fluorescence Imaging for Multianalyte Immunoassay

7.5 Perspective

Chapter 8. Fast Immunoassay

8.1 Mass transport-accelerated Immunosensing

8.2 Electric Field-Driven Strategy for Fast Immunoassay

8.3 Micro-bubble Acceleration for Fast Immunoassay

8.4 Infrared Heating for Flow Injection Immunoassay

8.5 Micro/Nano Motors for Fast Immunoassay

8.6 Perspective

Chapter 9. Proximity Hybridization Regulated Immunoassay

9.1 Proximity Hybridization Reaction

9.2 Ratiometric Electrochemical Proximity Immunoassay

9.3 Proximity Hybridization Regulated DNA Biogate

9.4 Target-driven Triple-binder Assembly of MNAzyme

9.5 Proximity-dependent DNAzyme for Immunoassay

9.6 Chemiluminescent Signal Switch Triggered by PHR

9.7 Perspective

Huangxian Ju

He received B.S., M.S. and Ph.D degrees from this university in 1986, 1989 and 1992, and became a full professor in 1999. He is a fellow of the International Society of Electrochemistry and the Royal Society of Chemistry, and won the National Funds for National Distinguished Young Scholars in 2003, and National Basic Research Program of China as a chief scientist in 2009. His research interests include analytical biochemistry and nanobiosensing, focusing on signal amplification and life analytical chemistry. He has guided about 40 students (1/3 of his students) to develop immunosensing methodology for detection of protein biomarkers with electrochemical biosensing, optical detection, imaging analysis, flow injection, biosensor array, molecular biology, signal amplification and nanobiotechnology.

Affiliations and expertise

Changjiang Scholar Professor of chemistry and Director, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University

Guosong Lai

Guosong Lai, graduated from Ju’s group with Ph.D degree in 2011, and is an associate professor of Chemistry in Hubei Normal University and named as Chutian Young Scholar in Hubei Province of China.

His research interests mainly focused on immunosensing and immunoassay methodology

Affiliations and expertise

Associate Professor of Chemistry, Hubei Normal University, China

Feng Yan

Feng Yan is a senior researcher at Jiangsu Institute of Cancer Prevention and Cure and a professor at Nanjing Medical University. She is also the deputy director of both the department of clinical laboratory and the Province Key Laboratory of Cancer Molecular Biology and Translational Medicine in Jiangsu Cancer Hospital, and the leading talent at Jiangsu medicine. Her research interest focuses on the methodology of molecular diagnosis and practical clinical application. She has published around 100 papers and 4 books with citations of over 2000.

Affiliations and expertise

Senior Researcher, Jiangsu Institute of Cancer Prevention and Cure and Professor, Nanjing Medical University; Deputy Director, Department of Clinical Laboratory and Province Key Laboratory of Cancer Molecular Biology and Translational Medicine, Jiangsu Cancer Hospital