Description

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • The third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells
  • The chapters provide hints and tricks not available in primary research publications
  • It is an invaluable resource for academics, researchers and students alike

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

Table of Contents

A: Viruses, Microbes and Parasites

Nanoscale imaging of microbial pathogens using atomic force microscopy

David

Alsteens

Challenges in imaging parasites

Friedrich

Frischknecht

Real-time live imaging to study bacterial infections in vivo

Agneta

Richter-Dahlfors

Analysis of cellular membrane dynamics by following virus endocytosis

Mario

Schelhaas

Imaging of live malaria blood stage parasites

Tobias

Spielmann

Escherichia coli K1 invasion of human brain microvascular endothelial cells

Theresa H.

Ward

B: Disease States

Real-time imaging after cerebral ischemia

Jasna

Kriz

Imaging acute inflammation in zebrafish

Graham J.

Lieschke

Imaging protein oligomerization in neurodegeneration using BiEC

Tiago Fleming

Outeiro

Imagng and quantifying the dyanmics of tumor-associated proteolysis

Bonnie F.

Sloane

C: Other Techniques

Live cell imaging in vivo with fluorescent proteins

Robert M.

Hoffman

Visualization of gene expression

Banafshe

Larijani

Imaging stem cell implant for cellular-based therapies

Zhenglong

Lee

Live cell single molecule imaging to investigate early events in B lymphocyte signaling

Susan K.

Pierce

A quantitative method for measuring phototoxicity of a live cell imaging microscope

Spencer L.

Shorte

High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines

Davide

Danovi

High-content screening of primary neurons

Steven

Finkbeiner

Live-imaging fluorescent prot

Details

No. of pages:
544
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123918680
Print ISBN:
9780123918567

About the serial-volume-editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve