Description

This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

Table of Contents

Series Page

Preface

Volume in series

Laser-Induced Radiation Microbeam Technology and Simultaneous Real-Time Fluorescence Imaging in Live Cells

1. Introduction

2. Experimental Procedure

3. Conclusion/Forward Look

Acknowledgments

A Cell Biologist's Guide to High Resolution Imaging

1. Introduction

2. Physical Limitations on the Resolution of Conventional Microscopy

3. Preparations for High Resolution Fluorescence Imaging

4. High Resolution Image Data Acquisition and Processing

5. Processing

6. Analysis of High Resolution Image Data

7. Conclusions

Acknowledgments

Applications of Fluorescence Lifetime Spectroscopy and Imaging to Lipid Domains In Vivo

1. The Challenge of Studying Lipid Domains In Vivo Defied by Fluorescence Lifetimes

2. Importance of Studies In Vitro to Design and Rationalize Studies In Vivo

3. Labeling Cell Membranes In Vivo: Lipophilic Probes Versus GFP-Tagged Membrane Proteins

4. Cuvette Lifetimes and FLIM: In Vivo Applications

Acknowledgments

Detecting and Tracking Nonfluorescent Nanoparticle Probes in Live Cells

1. Introduction

2. Techniques and Tools

3. Biological Applications

4. Cytotoxicity of Nanoparticle Probes

5. Conclusions and Future Perspective

Acknowledgments

Fluorescence Lifetime Microscopy of Tumor Cell Invasion, Drug Delivery, and Cytotoxicity

1. Introduction

2. Preparation of Mouse Mammary Tumor Cell Spheroids in a Collagen Matrix

3. Simultaneous Acquisition of Fluorescence Lifetime and Intensity to Monitor DOX Uptake

4. Monitoring Drug Uptake Kinetics by FLIM

5. Conclusions and Outlook

Acknowledgment

Measuring Membrane Protein Dynamics in Neurons Using Fluorescence Recovery after

Details

No. of pages:
560
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123918697
Print ISBN:
9780123918574

About the serial-volume-editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve