This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

    Table of Contents

    Series Page


    Volume in series

    Laser-Induced Radiation Microbeam Technology and Simultaneous Real-Time Fluorescence Imaging in Live Cells

    1. Introduction

    2. Experimental Procedure

    3. Conclusion/Forward Look


    A Cell Biologist's Guide to High Resolution Imaging

    1. Introduction

    2. Physical Limitations on the Resolution of Conventional Microscopy

    3. Preparations for High Resolution Fluorescence Imaging

    4. High Resolution Image Data Acquisition and Processing

    5. Processing

    6. Analysis of High Resolution Image Data

    7. Conclusions


    Applications of Fluorescence Lifetime Spectroscopy and Imaging to Lipid Domains In Vivo

    1. The Challenge of Studying Lipid Domains In Vivo Defied by Fluorescence Lifetimes

    2. Importance of Studies In Vitro to Design and Rationalize Studies In Vivo

    3. Labeling Cell Membranes In Vivo: Lipophilic Probes Versus GFP-Tagged Membrane Proteins

    4. Cuvette Lifetimes and FLIM: In Vivo Applications


    Detecting and Tracking Nonfluorescent Nanoparticle Probes in Live Cells

    1. Introduction

    2. Techniques and Tools

    3. Biological Applications

    4. Cytotoxicity of Nanoparticle Probes

    5. Conclusions and Future Perspective


    Fluorescence Lifetime Microscopy of Tumor Cell Invasion, Drug Delivery, and Cytotoxicity

    1. Introduction

    2. Preparation of Mouse Mammary Tumor Cell Spheroids in a Collagen Matrix

    3. Simultaneous Acquisition of Fluorescence Lifetime and Intensity to Monitor DOX Uptake

    4. Monitoring Drug Uptake Kinetics by FLIM

    5. Conclusions and Outlook


    Measuring Membrane Protein Dynamics in Neurons Using Fluorescence Recovery after


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    © 2012
    Academic Press
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