Hydrolytic Enzymes, Volume 16

1st Edition

Editors: K. Brocklehurst A. Neuberger
Hardcover ISBN: 9780444808868
eBook ISBN: 9780080860756
Imprint: Elsevier Science
Published Date: 1st March 1988
Page Count: 422
72.95 + applicable tax
43.99 + applicable tax
54.95 + applicable tax
Unavailable
Compatible Not compatible
VitalSource PC, Mac, iPhone & iPad Amazon Kindle eReader
ePub & PDF Apple & PC desktop. Mobile devices (Apple & Android) Amazon Kindle eReader
Mobi Amazon Kindle eReader Anything else

Institutional Access


Table of Contents

Chapter 1. Aspartyl proteinases (J.S. Fruton). Molecular properties. Action on Protein Substrates. Action on Synthetic Substrates. Mechanism of Action. Chapter 2. Cysteine proteinases (K. Brocklehurst, F. Willenbrock and E. Salih). The Cysteine Proteinases: General Considerations. An Historical Perspective from Papaine to Calpain. Physiological and Pathological Aspects. Isolation and Assay. Molecular Structure. Specificity. Chemical Modification. Spectroscopic Studies. Kinetics and Catalytic Mechanism. Chapter 3. Structure and function of serine proteases (L. Polgar). The Pre-crystallographic Age. Structural Features of the Catalytic Mechanism. Beyond the Structure. Conclusion. Chapter 4. Carboxypeptidase A (D.S. Auld and B.L. Vallee). Structural Properties. Catalytic Properties. Chemical Modification of Active Site Residues. Structure-Function Studies by Site-Directed Mutagenesis. Function of the Metal. The Chromophoric Cobalt Atom as an Active Site Probe. Cryospectrokinetic Studies. Hydrolysis of Peptide Substrates. Rate Determining Step in Ester and Peptide Hydrolysis. Relationship to Other Metalloproteases. Summary. Chapter 5. Proteinase Inhibitors (Y. Birk). Distribution and Occurrence. Classification and Nomenclature. Mechanism of Action of Proteinase Inhibitors. Plant Proteinase Inhibitors. Proteinase Inhibitors of Animal Origin. Concluding Remarks. Chapter 6. Intracellular proteolysis (P. Bohley). Turnover of Proteins in Organelles. Cell Proteinases. Substrate Properties Determining Proteolysis in Cells. Regulation of Intracellular Proteolysis. Concluding Remarks. Chapter 7. Pancreatic Ribonuclease A: The most studied endoribonuclease (M.R. Eftink and R.L. Biltonen). Biological Function and Occurrence of RNases. Structure of RNase A. Thermodynamics of Ligand Binding to RNase A. Kinetics. Miscellaneous Topics. Specificity, Mechanism, and Ener

Description

This newest volume in the impressive New Comprehensive Biochemistry series presents up-to-date discussions of six types of hydrolytic enzyme that are well characterized structurally: aspartic-, cysteins-, and serine-proteinases, carboxypeptidase A, pancreatic ribonuclease A, and the phosphomonoesterases. The emphasis is on molecular mechanisms deduced by crystallographic, kinetic, spectroscopic and molecular genetic studies. The chapters on the various types of proteinases are complemented by others on proteinase inhibitors and intracellular proteolysis.
This book will prove valuable to researchers in general biochemistry, particularly those with interest in enzyme mechanism and protein chemistry, and to Honours and Postgraduate students.


Details

No. of pages:
422
Language:
English
Copyright:
© Elsevier Science 1987
Published:
Imprint:
Elsevier Science
eBook ISBN:
9780080860756
Hardcover ISBN:
9780444808868

Reviews

This newest volume in the impressive New Comprehensive Biochemistry series presents up-to-date discussions of six types of hydrolytic enzyme that are well characterized structurally: aspartic-, cysteins-, and serine-proteinases, carboxypeptidase A, pancreatic ribonuclease A, and the phosphomonoesterases. The emphasis is on molecular mechanisms deduced by crystallographic, kinetic, spectroscopic and molecular genetic studies. The chapters on the various types of proteinases are complemented by others on proteinase inhibitors and intracellular proteolysis. This book will prove valuable to researchers in general biochemistry, particularly those with interest in enzyme mechanism and protein chemistry, and to Honours and Postgraduate students.


About the Editors

K. Brocklehurst Editor

A. Neuberger Editor