Key Features

  • Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications.
  • Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America.
  • Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

Readership

Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses

Table of Contents

Foreword 1

Foreword 2

Preface 1

Preface 2

List of Contributors

PART 1: Basic Methods

Chapter 1. Culturing Human Pluripotent Stem Cells on a Feeder Layer

Editor’s Commentary

Overview

Procedures

Alternative Procedures

Pitfalls and Advice

Equipment

Reagents and Supplies

Recipes

Reading List

Chapter 2. Preparation of Mouse Embryonic Fibroblast Feeder Cells

Editor’s Commentary

Overview

Procedures

Alternative Procedures

Pitfalls and Advice

Equipment

Reagents and Supplies

Recipes

Reading List

Chapter 3. Culture of Human Pluripotent Stem Cells in Feeder-Free Conditions

Editor’s Commentary

Overview

Procedures

Alternative Procedures

Pitfalls and Advice

Equipment

Reagents and Supplies

Recipes

Quality Control Methods

Reading List

Chapter 4. Contamination

Editor’s Commentary

Overview

Identifying Contamination

Controlling Contamination

Reagents and Supplies

Reading List

Chapter 5. Cell Culture Medium

Editor’s Commentary

Overview

Design of Culture Medium

Commercially Available Stem Cell Media

Assessing Medium Efficacy

Perspective

Reading List

Chapter 6. Cryopreservation of Human Embryonic Stem Cells

Editor’s Commentary

Overview

Procedures

Pitfalls and Advice

Alternative Procedures

Equipment

Reagents and Supplies

Recipes

Reading List

Chapter 7. Setting up a Research Scale Laboratory for Human Pluripotent Stem Cells

Editor’s Commentary

Overview

Laboratory

Record Keeping

Equipment Maintenance

Safety

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Details

No. of pages:
656
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123854742
Print ISBN:
9780123854735
Print ISBN:
9780128101018

About the editor