Human Stem Cell Manual

Human Stem Cell Manual

A Laboratory Guide

2nd Edition - August 1, 2012

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  • Editors: Suzanne Peterson, Jeanne Loring
  • eBook ISBN: 9780123854742
  • Hardcover ISBN: 9780123854735

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Description

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.

Key Features

  • Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications
  • Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America
  • Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs

Readership

Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses

Table of Contents

  • Foreword 1

    Foreword 2

    Preface 1

    Preface 2

    List of Contributors

    PART 1: Basic Methods

    Chapter 1. Culturing Human Pluripotent Stem Cells on a Feeder Layer

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 2. Preparation of Mouse Embryonic Fibroblast Feeder Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 3. Culture of Human Pluripotent Stem Cells in Feeder-Free Conditions

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    Chapter 4. Contamination

    Editor’s Commentary

    Overview

    Identifying Contamination

    Controlling Contamination

    Reagents and Supplies

    Reading List

    Chapter 5. Cell Culture Medium

    Editor’s Commentary

    Overview

    Design of Culture Medium

    Commercially Available Stem Cell Media

    Assessing Medium Efficacy

    Perspective

    Reading List

    Chapter 6. Cryopreservation of Human Embryonic Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Advice

    Alternative Procedures

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 7. Setting up a Research Scale Laboratory for Human Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Laboratory

    Record Keeping

    Equipment Maintenance

    Safety

    Quality Control

    Cell Banking

    Reading List

    Chapter 8. Biobanks for Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Banking of Human Pluripotent Stem Cells

    Stem Cell Providers

    Future of Banking and New iPSC Initiatives

    Reading List

    PART 2: Induced Pluripotent Stem Cell Technology

    Chapter 9. Isolation of Human Dermal Fibroblasts from Biopsies

    Editor’s Commentary

    Overview

    Procedure

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 10. Human Induced Pluripotent Stem Cell Generation

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Acknowledgments

    Reading List

    Chapter 11. Integration-Free Method for the Generation of Human Induced Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Acknowledgments

    Reading List

    Chapter 12. Methods for Evaluating Human Induced Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Equipment

    Reagents and Supplies

    Acknowledgments

    Reading List

    PART 3: Characterization of Human Pluripotent Stem Cells

    Chapter 13. Classical Cytogenetics

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Acknowledgments

    Reading List

    Chapter 14. SNP Genotyping to Detect Genomic Alterations in Human Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative procedures

    Pitfalls and Advice

    Equipment and Supplies

    Quality Control Methods

    Reading List

    Chapter 15. Analysis and Purification Techniques for Human Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Reading list

    Chapter 16. Immunocytochemical Analysis of Human Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Advice

    Digital Images of Fluorescent Cells

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 17. Analysis of Genome-Wide Gene Expression Data from Microarrays and Sequencing

    Editor’s Commentary

    Overview

    Experimental Design

    Critical Statistical Concepts

    Microarray Data Analysis

    RNA Sequencing (RNA-Seq)

    Pitfalls and Advice

    Reading List

    Chapter 18. PluriTest Molecular Diagnostic Assay for Pluripotency in Human Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Pluritest Output

    Novelty Score

    Density Plot

    Quality Control Outputs

    Data, Experimental Design, and Formats

    Pitfalls and Advice: Frequently Asked Questions

    Reading List

    Chapter 19. Epigenetics

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Equipment

    Reagents and Supplies

    Recipes

    ChIP Antibodies

    Quality Control Methods

    Reading List

    Chapter 20. Epigenetics

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment, Reagents, and Supplies

    Quality Control Methods

    Reading List

    Chapter 21. Generation of Human Pluripotent Stem Cell-Derived Teratomas

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Reading List

    Chapter 22. Characterization of Human Pluripotent Stem Cell-Derived Teratomas

    Editor’s Commentary

    Overview

    Procedures

    hiPSC-derived Teratomas

    Reading List

    PART 4: Differentiation

    Chapter 23. Spontaneous Differentiation of Human Pluripotent Stem Cells via Embryoid Body Formation

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 24. Differentiation of Human Pluripotent Stem Cells into Neural Progenitors

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 25. Dopaminergic Neuronal Differentiation of Human Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 26. Directed Differentiation of Human Pluripotent Stem Cells to Oligodendrocyte Progenitor Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    Chapter 27. Cardiomyocyte Differentiation of Human Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    Chapter 28. Directed Differentiation of Human Pluripotent Stem Cells into Fetal-Like Hepatocytes

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    PART 5: Genetic Manipulation

    Chapter 29. Transfection of Human Pluripotent Stem Cells by Electroporation

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    Chapter 30. Lentiviral Vector Systems for Transgene Delivery

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 31. Gene Targeting by Homologous Recombination in Human Pluripotent Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 32. Genetic Manipulation of Human Pluripotent Stem Cells Using Zinc Finger Nucleases

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Advice

    Reagents and Supplies

    Recipes

    Reading List

    PART 6: Stem Cell Transplantation

    Chapter 33. Intraspinal Transplantation of Human Neural Stem Cells

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    Chapter 34. Transplanting Stem Cells into the Brain

    Editor’s Commentary

    Overview

    Procedures

    Pitfalls and Caveats

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    PART 7: Derivation of Human Pluripotent Stem Cells

    Chapter 35. Development of Human Blastocysts

    Editor’s Commentary

    Overview

    Laws and Ethics

    Background

    Procedures

    Equipment

    Reagents and Supplies

    Recipes

    Reading List

    Chapter 36. Derivation of Human Embryonic Stem Cells from Blastocysts

    Editor’s Commentary

    Overview

    Procedures

    Alternative Procedures

    Pitfalls and Advice

    Equipment

    Reagents and Supplies

    Recipes

    Quality Control Methods

    Reading List

    PART 8: Stem Cells and Society

    Chapter 37. Stem Cell Patents

    Editor’s Commentary

    Overview

    What is a Patent?

    Obtaining a Patent on an Invention

    The America Invents Act of 2011

    Patent Licensing Policies

    Material Transfer

    Patent Pools

    Stem Cell Patents

    Human Embryonic Stem Cell Patents

    Induced Pluripotent Stem Cell Patents

    Reading List

    Chapter 38. Ethics of Human Pluripotent Stem Cell Research and Development

    Editor’s Commentary

    Overview

    Ethical Issues for Human Pluripotent Stem Cell Research

    Informed Consent

    Early Clinical Trials and Stem Cell Tourism

    Review Committees

    Scientific Responsibilities

    Reading List

    Index

Product details

  • No. of pages: 656
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: August 1, 2012
  • Imprint: Academic Press
  • eBook ISBN: 9780123854742
  • Hardcover ISBN: 9780123854735

About the Editors

Suzanne Peterson

Suzanne Peterson received her doctoral degree from USC and immediately moved back to her home town of San Diego to pursue her Post-Doctoral work. A long-time member and the only native San Diegan of the Loring Laboratory at Scripps Research Institute, this summer Dr. Peterson followed her mentor Dr. Loring to Aspen Neuroscience and will serve as a senior scientist in the new founded lab. Her specialty is hematopoietic stem cells and she will continue to serve as co-editor of Human Stem Cell Manual: A Laboratory Guide with Dr. Jeanne Loring.

Affiliations and Expertise

This summer Dr. Peterson followed her mentor Dr. Loring to Aspen Neuroscience and will serve as a senior scientist in the new founded lab.

Jeanne Loring

Jeanne Loring is a world renown stem cell biologist, developmental neurobiologist, and geneticist. Dr. Loring’s 3+ decades have spanned embryology and neurobiology, gene editing and stem cells, ultimately landing in regenerative medicine. Her decades of experience ranges from biotech, to pharma to bioscience and of course academia. She was the director for the Center for Regenerative Medicine and professor at Scripps Research Institute in La Jolla, CA for 12+ years. In June 2019 she founded Aspen Neuroscience, in La Jolla, where she and her staff will continue her work in regenerative medicine for those with currently untreatable neurological diseases. Their first target is a patient-specific neuron replacement therapy for Parkinson’s disease. She will continue as Professor Emeritus and remains on the faculty of Scripps Research Institute. Dr. Loring has received numerous awards and accolades, including the 2015 Stem Cell Action Advocacy Award and 2015 Stem Cell Person of the Year.

Affiliations and Expertise

The Scripps Research Institute, La Jolla, California, U.S.A.

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