Human Stem Cell Manual

2nd Edition

A Laboratory Guide

Editors: Suzanne Peterson Jeanne Loring
Hardcover ISBN: 9780123854735
eBook ISBN: 9780123854742
Imprint: Academic Press
Published Date: 1st August 2012
Page Count: 656
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Description

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work.  The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.

Key Features

  • Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications.
  • Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America.
  • Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

Readership

Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses

Table of Contents

Foreword 1

Foreword 2

Preface 1

Preface 2

List of Contributors

PART 1: Basic Methods

Chapter 1. Culturing Human Pluripotent Stem Cells on a Feeder Layer

Editor’s Commentary

Overview

Procedures

Alternative Procedures

Pitfalls and Advice

Equipment

Reagents and Supplies

Recipes

Reading List

Chapter 2. Preparation of Mouse Embryonic Fibroblast Feeder Cells

Editor’s Commentary

Overview

Procedures

Alternative Procedures

Pitfalls and Advice

Equipment

Reagents and Supplies

Recipes

Reading List

Chapter 3. Culture of Human Pluripotent Stem Cells in Feeder-Free Conditions

Editor’s Commentary

Overview

Procedures

Alternative Procedures

Pitfalls and Advice

Equipment

Reagents and Supplies

Recipes

Quality Control Methods

Reading List

Chapter 4. Contamination

Editor’s Commentary

Overview

Identifying Contamination

Controlling Contamination

Reagents and Supplies

Reading List

Chapter 5. Cell Culture Medium

Editor’s Commentary

Overview

Design of Culture Medium

Commercially Available Stem Cell Media

Assessing Medium Efficacy

Perspective

Reading List

Chapter 6. Cryopreservation of Human Embryonic Stem Cells

Editor’s Commentary

Overview

Procedures

Pitfalls and Advice

Alternative Procedures

Equipment

Reagents and Supplies

Recipes

Reading List

Chapter 7. Setting up a Research Scale Laboratory for Human Pluripotent Stem Cells

Editor’s Commentary

Overview

Laboratory

Record Keeping

Equipment Maintenance

Safety

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Details

No. of pages:
656
Language:
English
Copyright:
© Academic Press 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780123854742
Hardcover ISBN:
9780123854735
Paperback ISBN:
9780128101018

About the Editor

Suzanne Peterson

Affiliations and Expertise

The Scripps Research Institute, La Jolla, CA

Jeanne Loring

Affiliations and Expertise

The Burnham Institute, La Jolla, California, U.S.A.

Reviews

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work.  The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.