Human Stem Cell Manual

Human Stem Cell Manual

A Laboratory Guide

1st Edition - June 1, 2007

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  • Editors: Suzanne Peterson, Jeanne Loring, Robin Wesselschmidt, Philip Schwartz
  • eBook ISBN: 9780080549880

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Description

Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.

Readership

Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses.

Table of Contents

  • Foreword by James Battey
    Foreword by Jeannie Fontana
    Preface
    Part I: Basic methods: Maintenance of stem cell cultures
    1.Human Embryonic Stem Cell Culture
    2.Feeder layers and feeder-free culture
    3.Mouse Embryonic Fibroblast Feeder Cells
    4.Cryopreservation of hESC

    Part II: Basic methods: Characterization of stem cells
    5.Classical Cytogenetics: Karyotyping
    6.Spectral Karyotyping and Fluorescent in situ Hybridization
    7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
    8.FACS/Cell sorting
    9.Fluorescence Immunocytochemical Analysis of Stem Cells
    10.Characterization of Stem Cells Using RT-PCR
    11.Gene Expression Profiling of Stem Cells by Microarray
    12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice
    13.Generation of HESC-Derived Teratomas
    Part III: Differentiation of stem cells
    14.“Embryoid Body” Formation and Neuroepithelia Differentiation
    15.Motor Neuron and Dopamine Neuron Differentiation
    16.Oligodendrocyte Differentiation from hESC
    17.Cardiac
    18.Hematopoietic
    Part IV: Genetic manipulation of stem cells
    19.Genetic Manipulation of hESC: Lentivirus Vectors
    20.Methods developed for mouse ES cells
    Part V: Advanced methods
    21.Derivation of human ES cells
    22.Neural Stem Cell Culture
    23.Transplantation of stem cells
    24.In vitro fertilization
    25.Functional characterization-neural cells
    Part VI: Practical issues in establishing a human ES cell laboratory
    26.Setting Up a Facility for hESC Research

    Part VII: Essays on stem cell biology
    27.Intellectual Property: Owning the Stem Cell
    28.Ethical Concerns for Stem Cell Research
    29.Guidelines for hESC Research Oversight (ESCRO) Committees

Product details

  • No. of pages: 488
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: June 1, 2007
  • Imprint: Academic Press
  • eBook ISBN: 9780080549880

About the Editors

Suzanne Peterson

Suzanne Peterson received her doctoral degree from USC and immediately moved back to her home town of San Diego to pursue her Post-Doctoral work. A long-time member and the only native San Diegan of the Loring Laboratory at Scripps Research Institute, this summer Dr. Peterson followed her mentor Dr. Loring to Aspen Neuroscience and will serve as a senior scientist in the new founded lab. Her specialty is hematopoietic stem cells and she will continue to serve as co-editor of Human Stem Cell Manual: A Laboratory Guide with Dr. Jeanne Loring.

Affiliations and Expertise

This summer Dr. Peterson followed her mentor Dr. Loring to Aspen Neuroscience and will serve as a senior scientist in the new founded lab.

Jeanne Loring

Jeanne Loring is a world renown stem cell biologist, developmental neurobiologist, and geneticist. Dr. Loring’s 3+ decades have spanned embryology and neurobiology, gene editing and stem cells, ultimately landing in regenerative medicine. Her decades of experience ranges from biotech, to pharma to bioscience and of course academia. She was the director for the Center for Regenerative Medicine and professor at Scripps Research Institute in La Jolla, CA for 12+ years. In June 2019 she founded Aspen Neuroscience, in La Jolla, where she and her staff will continue her work in regenerative medicine for those with currently untreatable neurological diseases. Their first target is a patient-specific neuron replacement therapy for Parkinson’s disease. She will continue as Professor Emeritus and remains on the faculty of Scripps Research Institute. Dr. Loring has received numerous awards and accolades, including the 2015 Stem Cell Action Advocacy Award and 2015 Stem Cell Person of the Year.

Affiliations and Expertise

The Scripps Research Institute, La Jolla, California, U.S.A.

Robin Wesselschmidt

Philip Schwartz

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