Human Chromosome Methodology - 1st Edition - ISBN: 9781483231945, 9781483266312

Human Chromosome Methodology

1st Edition

Editors: Jorge J. Yunis
eBook ISBN: 9781483266312
Imprint: Academic Press
Published Date: 1st January 1965
Page Count: 272
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Human Chromosome Methodology fills the need for an authoritative and up-to-date treatise which would serve as a text and reference for advances in human cytogenetics. The book includes readily comprehensible chapters that cover each phase of laboratory investigation from the preparation of materials for sex chromatin and chromosome techniques for bone marrow, blood, skin, and gonadal specimens to the subject of autoradiography and chromosome identification. Included also are guides to microscopy and photomicrography as well as an up-to-date treatment of chromosomes in disease.
It is hoped that this volume will serve as an adequate guide to laboratory techniques and their applications for research workers, students of genetics, and members of the medical profession involved in setting up a laboratory of cytogenetics.

Table of Contents

Preface Introduction Sex Chromatin Techniques

1. Introduction II. Historical Sketch III. Morphological Characteristics of the Sex Chromatin IV. Sex Chromatin Techniques V. Origin of the Sex Chromatin VI. Sex Chromatin Patterns and Sex Chromosome Complexes References

Human Peripheral Blood Leucocyte Cultures I. General II. The Mononuclear Leucocyte as the Mitotic Cell in Culture III. Collection of Peripheral Blood Leucocytes and Preparation of Cell Inoculum IV. Initiation of Mitosis in Blood Cultures: The Role of Phytohemagglutinin and Other Potentially Mitogenic Agents V. Influence of Culture Conditions on Cell Proliferation VI. Preparation of Metaphase Spreads for Cytogenic Studies VII. Life Span of PHA Initiated Cultures: Evidence for Secondary Mitoses in Culture VIII. Further Application of the Leucocyte Culture System IX. Methods for Culturing Human Peripheral Blood Leucocytes References Direct Chromosome Preparations of Bone Marrow Cells I. Introduction II. Procedures III. Discussion References The Skin Culture Technique I. Introduction II. Media and Materials III. Step by Step Procedure IV. General Discussion of Method Appendix References Direct Handling of Germ Cells I. Introduction II. Fixing, Squashing, and Staining Gonadal Material III. Various Methods of Pretreatment IV. Interpreting the Findings V. Summary References Autoradiography of Human Chromosomes I. Introduction II. Methodology III. Synopsis and Sequence of Technical Procedures for Autoradiography of Human Chromosomes IV. Techniques Appendix References Bright Field, Phase Contrast, and Fluorescence Microscopy I. Introduction II. Theory of Light Microscopes III. Properties of Microscopic Lens Systems: Bright Field Microscopy IV. Phase


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© Academic Press 1965
Academic Press
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About the Editor

Jorge J. Yunis

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