Human Chromosome Methodology

1st Edition - January 1, 1965
Editor: Jorge J. Yunis
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 6 6 3 1 - 2

Human Chromosome Methodology fills the need for an authoritative and up-to-date treatise which would serve as a text and reference for advances in human cytogenetics. The book… Read more

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Human Chromosome Methodology fills the need for an authoritative and up-to-date treatise which would serve as a text and reference for advances in human cytogenetics. The book includes readily comprehensible chapters that cover each phase of laboratory investigation from the preparation of materials for sex chromatin and chromosome techniques for bone marrow, blood, skin, and gonadal specimens to the subject of autoradiography and chromosome identification. Included also are guides to microscopy and photomicrography as well as an up-to-date treatment of chromosomes in disease. It is hoped that this volume will serve as an adequate guide to laboratory techniques and their applications for research workers, students of genetics, and members of the medical profession involved in setting up a laboratory of cytogenetics.

Preface

Introduction

Sex Chromatin Techniques

1. Introduction

II. Historical Sketch

III. Morphological Characteristics of the Sex Chromatin

IV. Sex Chromatin Techniques

V. Origin of the Sex Chromatin

VI. Sex Chromatin Patterns and Sex Chromosome Complexes

References

Human Peripheral Blood Leucocyte Cultures

I. General

II. The Mononuclear Leucocyte as the Mitotic Cell in Culture

III. Collection of Peripheral Blood Leucocytes and Preparation of Cell Inoculum

IV. Initiation of Mitosis in Blood Cultures: The Role of Phytohemagglutinin and Other Potentially Mitogenic Agents

V. Influence of Culture Conditions on Cell Proliferation

VI. Preparation of Metaphase Spreads for Cytogenic Studies

VII. Life Span of PHA Initiated Cultures: Evidence for Secondary Mitoses in Culture

VIII. Further Application of the Leucocyte Culture System

IX. Methods for Culturing Human Peripheral Blood Leucocytes

References

Direct Chromosome Preparations of Bone Marrow Cells

I. Introduction

II. Procedures

III. Discussion

References

The Skin Culture Technique

I. Introduction

II. Media and Materials

III. Step by Step Procedure

IV. General Discussion of Method

Appendix

References

Direct Handling of Germ Cells

I. Introduction

II. Fixing, Squashing, and Staining Gonadal Material

III. Various Methods of Pretreatment

IV. Interpreting the Findings

V. Summary

References

Autoradiography of Human Chromosomes

I. Introduction

II. Methodology

III. Synopsis and Sequence of Technical Procedures for Autoradiography of Human Chromosomes

IV. Techniques

Appendix

References

Bright Field, Phase Contrast, and Fluorescence Microscopy

I. Introduction

II. Theory of Light Microscopes

III. Properties of Microscopic Lens Systems: Bright Field Microscopy

IV. Phase Contrast Microscopy

V. Fluorescence Microscopic Technique

References

Applied Photography in Chromosome Studies

I. Introduction

II. Photomicrography

III. Photosensitive Materials and Processing

Appendix

References

Identification of Chromosomes

I. Morphological Identification in the Absence of Special Markers

II. Markers and Other Aids to Chromosome Identification

III. Nomenclature

IV. The Human Complement

References

Human Chromosomes in Disease

I. Introduction

II. Sex Chromosomal Abnormalities

III. Autosomal Chromosome Abnormalities

IV. Chromosomes in Malignant Diseases

V. Diseases with a Normal Chromosome Complement

VI. Conclusion

References

Author Index

Subject Index