Human Chromosome Methodology
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Human Chromosome Methodology serves as an authoritative guide to cytogenetic techniques. This book presents each phase of laboratory work from preparation of materials for the X and Y bodies to application of other laboratory techniques including chromosome identification, autoradiography, and dermatoglyphics. The text also describes the structure and molecular organization of chromosomes and the advances in the automation of chromosome analysis. It provides a thorough review of the clinical manifestations of chromosome disorders. Organized into 13 chapters, the book presents the illustrated and diagrammatic examples and discussions of the subject matter and detailed tables and charts for learning efficiency. It also provides outlined presentation of cytogenetic procedures and notes and comments for each procedure that will assist readers in erroneous work phases. Moreover, it gives thorough lists of references in each chapter for further reading. This reference will be useful for research professionals, lecturers, genetics and molecular biology students, and members of the medical profession involved in genetics.
Table of Contents
List of Contributors
Preface
Preface to First Edition
1 Structure and Molecular Organization of Chromosomes
Text
References
2 Identification of Human Chromosomes
I. Introduction
II. Terms Used in the Identification of Human Chromosomes
III. Standardization of Nomenclature
IV. Recommendations of the Paris Conference
V. The Use of the Paris Conference Nomenclature in Clinical Cytogenetics
References
3 Quinacrine Fluorescent Patterns
I. Introduction
II. Procedure
References
4 Staining Constitutive Heterochromatin and Giemsa Crossbands of Mammalian Chromosomes
I. Introduction
II. Cell Harvest and Slide Preparations
III. C-Banding
IV. G-Banding
References
5 Sex Chromatin Bodies
I. Introduction
II. Discovery and Nomenclature
III. Appearance of Barr Bodies
IV. Numbers of Barr Bodies per Cell
V. Origin and Nature of Barr Bodies
VI. Appearance and Nature of Drumsticks
VII. Fluorescent Y Bodies
VIII. Techniques
References
6 Human Peripheral Blood Leukocyte Cultures
I. General
II. The Mononuclear Leukocyte as the Mitotic Cell in Culture
III. Collection of Peripheral Blood Leukocytes and Preparation of Cell Inoculum
IV. Initiation of Mitosis in Blood Cultures: The Role of Phytohemagglutinin and Other Potentially Mitogenic Agents
V. Influence of Culture Conditions on Cell Proliferation
VI. Preparation of Metaphase Spreads for Cytogenetic Studies
VII. Life Span of PHA-Initiated Cultures: Evidence for Secondary Mitoses in Culture
VIII. Further Application of the Leukocyte Culture System
IX. Methods for Culturing Human Peripheral Blood Leukocytes
References
7 Autoradiography of Human Chromosomes
I. Introduction
II. Principles of Methodology
III. Autoradiography in Combination with Other Techniques
IV. The Study of DNA Synthesis with Precursors Other Than Thymidine and of the Synthesis of RNA and Chromosomal Proteins
V. Technical Schedules
VI. Equipment and Materials for Autoradiography
References
8 Direct Chromosome Preparation of Bone Marrow Cells
I. Introduction
II. Procedures
III. Discussion
References
9 Skin Culture and Solid Tumor Technique
I. Introduction
II. Skin Culture
III. Solid Tumor Techniques
Appendix: Notes on Media and Materials
References
10 Amniotic Cell Culture
I. Introduction
II. Methodology
III. Discussion
References
11 Clinical Manifestations of Chromosome Disorders
I. Introduction
II. Autosomal Chromosome Abnormalities
III. Sex Chromosomal Abnormalities
IV. Chromosome Abnormalities in Spontaneous Abortion
V. Chromosomes and Cancer
VI. Diseases Associated with Spontaneous Chromosome Aberrations
VII. Radiation, Viruses, and Chemical Clastogens
References
12 Dermatoglyphics and Chromosomal Aberrations
I. Introduction
II. Classification of Dermatoglyphics
III. Flexion Creases
IV. Recording Dermatoglyphics and Creases
V. Dermatoglyphics in Clinical Disorders
VI. Clinical Applications of Dermatoglyphics: Advantages and Limitations
References
13 Chromosome Identification by Image Analysis and Quantitative Cytochemistry
I. Introduction
II. Cytochemistry
III. Scanning
IV. Image Analysis
V. Results
VI. Conclusion and Perspectives
Appendix: Staining Procedures Used for DNA Cytophotometry of Metaphase Chromosomes
References
Author Index
Subject Index
Product details
- No. of pages: 396
- Language: English
- Copyright: © Academic Press 1974
- Published: September 28, 1974
- Imprint: Academic Press
- eBook ISBN: 9780323161794