Human Chromosome Methodology

Human Chromosome Methodology

2nd Edition - September 28, 1974

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  • Author: Jorge J. Yunis
  • eBook ISBN: 9780323161794

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Human Chromosome Methodology serves as an authoritative guide to cytogenetic techniques. This book presents each phase of laboratory work from preparation of materials for the X and Y bodies to application of other laboratory techniques including chromosome identification, autoradiography, and dermatoglyphics. The text also describes the structure and molecular organization of chromosomes and the advances in the automation of chromosome analysis. It provides a thorough review of the clinical manifestations of chromosome disorders. Organized into 13 chapters, the book presents the illustrated and diagrammatic examples and discussions of the subject matter and detailed tables and charts for learning efficiency. It also provides outlined presentation of cytogenetic procedures and notes and comments for each procedure that will assist readers in erroneous work phases. Moreover, it gives thorough lists of references in each chapter for further reading. This reference will be useful for research professionals, lecturers, genetics and molecular biology students, and members of the medical profession involved in genetics.

Table of Contents

  • List of Contributors


    Preface to First Edition

    1 Structure and Molecular Organization of Chromosomes



    2 Identification of Human Chromosomes

    I. Introduction

    II. Terms Used in the Identification of Human Chromosomes

    III. Standardization of Nomenclature

    IV. Recommendations of the Paris Conference

    V. The Use of the Paris Conference Nomenclature in Clinical Cytogenetics


    3 Quinacrine Fluorescent Patterns

    I. Introduction

    II. Procedure


    4 Staining Constitutive Heterochromatin and Giemsa Crossbands of Mammalian Chromosomes

    I. Introduction

    II. Cell Harvest and Slide Preparations

    III. C-Banding

    IV. G-Banding


    5 Sex Chromatin Bodies

    I. Introduction

    II. Discovery and Nomenclature

    III. Appearance of Barr Bodies

    IV. Numbers of Barr Bodies per Cell

    V. Origin and Nature of Barr Bodies

    VI. Appearance and Nature of Drumsticks

    VII. Fluorescent Y Bodies

    VIII. Techniques


    6 Human Peripheral Blood Leukocyte Cultures

    I. General

    II. The Mononuclear Leukocyte as the Mitotic Cell in Culture

    III. Collection of Peripheral Blood Leukocytes and Preparation of Cell Inoculum

    IV. Initiation of Mitosis in Blood Cultures: The Role of Phytohemagglutinin and Other Potentially Mitogenic Agents

    V. Influence of Culture Conditions on Cell Proliferation

    VI. Preparation of Metaphase Spreads for Cytogenetic Studies

    VII. Life Span of PHA-Initiated Cultures: Evidence for Secondary Mitoses in Culture

    VIII. Further Application of the Leukocyte Culture System

    IX. Methods for Culturing Human Peripheral Blood Leukocytes


    7 Autoradiography of Human Chromosomes

    I. Introduction

    II. Principles of Methodology

    III. Autoradiography in Combination with Other Techniques

    IV. The Study of DNA Synthesis with Precursors Other Than Thymidine and of the Synthesis of RNA and Chromosomal Proteins

    V. Technical Schedules

    VI. Equipment and Materials for Autoradiography


    8 Direct Chromosome Preparation of Bone Marrow Cells

    I. Introduction

    II. Procedures

    III. Discussion


    9 Skin Culture and Solid Tumor Technique

    I. Introduction

    II. Skin Culture

    III. Solid Tumor Techniques

    Appendix: Notes on Media and Materials


    10 Amniotic Cell Culture

    I. Introduction

    II. Methodology

    III. Discussion


    11 Clinical Manifestations of Chromosome Disorders

    I. Introduction

    II. Autosomal Chromosome Abnormalities

    III. Sex Chromosomal Abnormalities

    IV. Chromosome Abnormalities in Spontaneous Abortion

    V. Chromosomes and Cancer

    VI. Diseases Associated with Spontaneous Chromosome Aberrations

    VII. Radiation, Viruses, and Chemical Clastogens


    12 Dermatoglyphics and Chromosomal Aberrations

    I. Introduction

    II. Classification of Dermatoglyphics

    III. Flexion Creases

    IV. Recording Dermatoglyphics and Creases

    V. Dermatoglyphics in Clinical Disorders

    VI. Clinical Applications of Dermatoglyphics: Advantages and Limitations


    13 Chromosome Identification by Image Analysis and Quantitative Cytochemistry

    I. Introduction

    II. Cytochemistry

    III. Scanning

    IV. Image Analysis

    V. Results

    VI. Conclusion and Perspectives

    Appendix: Staining Procedures Used for DNA Cytophotometry of Metaphase Chromosomes


    Author Index

    Subject Index

Product details

  • No. of pages: 396
  • Language: English
  • Copyright: © Academic Press 1974
  • Published: September 28, 1974
  • Imprint: Academic Press
  • eBook ISBN: 9780323161794

About the Author

Jorge J. Yunis

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