Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques

The Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques: Kidney, Lung, Heart, Intestine and Mesenteric Lymph Node provides a thoroughly illustrated review of the tissue damage and reparative changes associated with standardized irradiation doses in rhesus macaques. In addition to time-sequenced, routinely stained histologic sections, the book presents results of numerous histochemical, immunohistochemical and chromogenic in-situ stains that provide insight into the pathogenesis of radiation-associated tissue injury and repair.

This book is compiled and written by a board-certified veterinary pathologist with more than 40 years of experience in the interpretation of experimentally induced tissue alterations in laboratory animals. This experience included three years of service as facility pathologist at the Armed Forces Radiobiology Research Institute and in subsequent years providing histopathology interpretations on studies associated with development of therapeutics for radiation-associated injury. The combination of broad experience in pharmaceutical development coupled with specific experience with radiation-associated injury allows unique interpretive insights into the radiation-associated tissue changes presented in the Atlas.

The Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques is an important resource for researchers in the field of animal science radiation injury, including radiation oncologists and individuals involved in disaster preparedness related to accidental or deliberate radiation exposure of large populations.

• Addresses the chronological sequence of tissue alterations following defined radiation doses in rhesus macaques
• Provides photo-documentation of a broad range of histochemical and immunohistochemical stains that illustrate tissue damage and reparative changes following irradiation
• Provides insights into the involvement of various signaling and effector molecules in the development of radiation-associated injury

Researchers in nonhuman primate radiation (animal science and animal oncology). Radiation oncologists seeking translatable research for humans

Chapter 1: Kidney
Routine histology stain-
Hematoxylin & eosin
Histochemical stains-
Periodic acid-Schiff/hematoxylin
Phosphotungstic acid/hematoxylin
Chromotrope-aniline blue
Masson’s trichrome
Immunohistochemical stains-
von Willebrand factor
Collagen 1
Alpha smooth muscle actin
Connective tissue growth factor
CD3
CD20
CD31
CD38
CD68
CD163
Myeloperoxidase
Tryptase
Galectin-3
Pro-renin receptor

Chapter 2: Lung
Routine histology stain-
Hematoxylin & eosin
Histochemical stains-
Masson’s trichrome
Toluidine blue
Immunohistochemical stains-
Collagen 1
Alpha smooth muscle actin
Connective tissue growth factor
CD31
CD163
CD206
Myeloperoxidase
Tryptase
Galectin-3
Transforming growth factor-beta
Caspase 3

Chapter 3: Heart
Routine histology stain-
Hematoxylin & eosin
Histochemical stains-
Masson’s trichrome
Immunohistochemical stains-
Caspase 3
Galectin-3
Alpha smooth muscle actin

Chapter 4: Jejunum
Routine histology stain-
Hematoxylin & eosin
Histochemical stains-
Masson’s trichrome
Alcian blue/periodic acid-Schiff
Immunohistochemical stains-
MxA
Ki67
Connective tissue growth factor
Collagen 1
CD13
CD163
Lipopolysaccharide

Chapter 5: Colon
Routine histology stain-
Hematoxylin & eosin
Histochemical stains-
Masson’s trichrome
Alcian blue/periodic acid-Schiff
Immunohistochemical stains
Ki67
Collagen 1
Connective tissue growth factor
CD163
Lipopolysaccharide

Chapter 6: Mesenteric lymph node
Routine histology stain-
Hematoxylin & eosin
Histochemical stains-
Masson’s trichrome
Immunohistochemical stains-
Collagen 1
Myeloperoxidase
MxA
Lipopolysaccharide
Transforming growth factor-beta
Tryptase
CD163