High-Resolution Electrophoresis and Immunofixation

High-Resolution Electrophoresis and Immunofixation

Techniques and Interpretation

1st Edition - April 3, 1987

Write a review

  • Author: David F. Keren
  • eBook ISBN: 9781483165264

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


High-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation acquaints the reader with the available methodologies and instrumentation for performing high-resolution electrophoresis and immunofixation. This text presents the use of procedures of high-resolution electrophoresis and immunofixation to facilitate the diagnosis of a variety of serum protein abnormalities. It also provides case examples to illustrate the uses of these procedures and their possible caveats. The book contains chapters devoted to the methods and rationale for high-resolution electrophoresis; interpretation of high-resolution electrophoresis patterns in serum, urine, and cerebrospinal fluid; the immunofixation technique; and strategies for diagnosing monoclonal gammopathies. Medical technologists, physicians, and pathologists will find the book invaluable.

Table of Contents

  • Preface

    1. Methods and Rationale for High-Resolution Electrophoresis

    Review of Protein Structure and Electrophoretic Techniques

    Methods of High-Resolution Electrophoresis

    High-Resolution Electrophoresis versus Standard Electrophoresis


    2. Serum Proteins Identified by High-Resolution Electrophoresis

    Proteins Identified by High-Resolution Electrophoresis

    Alpha Region

    Inter-Alpha-1, -Alpha-2 Region

    Beta Region

    Gamma Region


    3. Interpretation of High-Resolution Electrophoresis Patterns in Serum, Urine, and Cerebrospinal Fluid

    Approach to Pattern Interpretation

    Interpretation of the Individual Patient's Sample

    Pattern Interpretation in Cerebrospinal Fluid

    HRE Interpretation for Urine


    4. Immunofixation Technique

    Principles of Immunoprecipitation


    Limitations of Immunoelectrophoresis


    Limitations of Immunofixation

    Cost Considerations


    5. Strategies for Diagnosing Monoclonal Gammopathies

    Ontogeny of B Lymphocytes

    T-Lymphocyte Development

    Conditions Associated with Monoclonal Gammopathies

    Monoclonal Gammopathies in Patients with B-Lymphocyte Neoplasms

    Laboratory Diagnosis of Monoclonal Gammopathies


    6. Case Studies for Interpretation

    General Comments about Interpretation


Product details

  • No. of pages: 248
  • Language: English
  • Copyright: © Butterworth-Heinemann 1987
  • Published: April 3, 1987
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483165264

About the Author

David F. Keren

Ratings and Reviews

Write a review

There are currently no reviews for "High-Resolution Electrophoresis and Immunofixation"