High-Performance Liquid Chromatography of Proteins and Peptides

High-Performance Liquid Chromatography of Proteins and Peptides

Proceedings of the first International Symposium

1st Edition - January 28, 1983

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  • Editor: Milton Hearn
  • eBook ISBN: 9780323157704

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Description

High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.

Table of Contents


  • Contributors

    Preface

    High-Performance Ion-Exchange Chromatography of Proteins: The Current Status

    Analysis of Peptic Fragmentation of Human Immunoglobulin G Using High-Performance Liquid Chromatography

    Cord Blood Screening for Hemoglobin Disorders by High-Performance Liquid Chromatography

    A Simple and Rapid Purification of Commercial Trypsin and Chymotrypsin by Reverse-Phase High-Performance Liquid Chromatography

    Adaptation of Reverse-Phase High-Performance Liquid Chromatography for the Isolation and Sequence Analysis of Peptides from Plasma Amyloid P-Component

    Characterization of Human Alcohol Dehydrogenase Isoenzymes by High-Performance Liquid Chromatographic Peptide Mapping

    Purification of Glycopeptides of Human Ceruloplasmin and Immunoglobulin D by High-Pressure Liquid Chromatography

    High-Performance Size-Exclusion Chromatography of Hydrolyzed Plant Proteins

    The Isolation of Peptides by High-Performance Liquid Chromatography Using Predicted Elution Positions

    The Application of High-Performance Liquid Chromatography for the Resolution of Proteins Encoded by the Human Adenovirus Type 2 Cell Transformation Region

    The Importance of Silica Type for Reverse-Phase Protein Separations

    Variables in the High-Pressure Cation-Exchange Chromatography of Proteins

    Application of High-Performance Liquid Chromatography to Competitive Labeling Studies: The Chemical Properties of Functional Groups of Glucagon

    Purification by Reverse-Phase High-Performance Liquid Chromatography of an Epidermal Growth Factor-Dependent Transforming Growth Factor

    Preparative Size-Exclusion Chromatography of Human Serum Apolipoproteins Using an Analytical Liquid Chromatograph

    Use of Reverse-Phase High-Performance Liquid Chromatography in Structural Studies of Neurophysins, Photolabeled Derivatives, and Biosynthetic Precursors

    Purification of Radiolabeled and Native Polypeptides by Gel Permeation High-Performance Liquid Chromatography

    Nonideal Size-Exclusion Chromatography of Proteins: Effects of pH at Low Ionic Strength

    Factors Influencing Chromatography of Proteins on Short Alkylsilane-Bonded Large Pore-Size Silicas

    High-Performance Liquid Chromatography Analysis in the Synthesis, Characterization, and Reactions of Neoglycopeptides

    Use of Size-Exclusion High-Performance Liquid Chromatography for the Analysis of the Activation of Prothrombin

    High-Performance Liquid Chromatography of Proteins: Purification of the Acidic Isozyme of Adenylosuccinate Synthetase from Rat Liver

    Application of High-Performance Liquid Chromatographic Peptide Purification to Protein Microsequencing by Solid-Phase Edman Degradation

    Measurement of Endogenous Leucine Enkephalin in Canine Caudate Nuclei and Hypothalami with High-Performance Liquid Chromatography and Field-Desorption Mass Spectrometry

    Fluorescent Techniques for the Selective Detection of Chromatographically Separated Peptides

    High-Performance Liquid Chromatographie Purification of Peptide Hormones: Ovine Hypothalamic Amunine (Corticotropin Releasing Factor)

    Isolation and Purification of Escherichia coli Heat-Stable Enterotoxin of Porcine Origin

    α-H-Acetyl-ß-Endorphin1-26 from the Neurointermediary Lobe of the Rat Pituitary: Isolation, Purification, and Characterization by High-Performance Liquid Chromatography

    Index

Product details

  • No. of pages: 284
  • Language: English
  • Copyright: © Academic Press 1983
  • Published: January 28, 1983
  • Imprint: Academic Press
  • eBook ISBN: 9780323157704

About the Editor

Milton Hearn

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