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Handbook of Solid Phase Microextraction - 1st Edition - ISBN: 9780124160170, 9780123914491

Handbook of Solid Phase Microextraction

1st Edition

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Editor: Janusz Pawliszyn
eBook ISBN: 9780123914491
Imprint: Elsevier
Published Date: 1st December 2011
Page Count: 496
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The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a "green" technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis. This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed.

This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method.

Key Features

  • Practical application of Solid Phase Microextraction methods including detailed steps
  • Provides history of extraction methods to better understand the process
  • Suitable for all levels, from beginning student to experienced practitioner


Chemists and chemistry students at postgraduate and undergraduate levels

Table of Contents



List of Contributors

1. Solid-Phase Microextraction in Perspective

1.1. Sample Preparation as Part of the Analytical Process

1.2. Classification of Extraction Techniques

1.3. Perspective on Microextraction Techniques

1.4. Implementations of SPME

1.5. Miniaturisation and Integration

1.6. In Vivo Analysis

1.7. SPME Versus SPE

2. Theory of Solid-Phase Microextraction

2.1. Introduction

2.2. SPME Principle

2.3. Thermodynamics

2.4. Kinetics

2.5. Extraction with Derivatisation

2.6. Extraction of Sample Matrices Containing Solids

2.7. Solid Versus Liquid Sorbents

2.8. Passive TWA Sampling

2.9. In-Tube SPME

2.10. Experimental Verification

3. Development of SPME Devices and Coatings

3.1. Historical Perspective

3.2. Rational Design of SPME Devices

3.3. On-Site Samplers

3.4. Development of New SPME Coatings

3.5. Interfaces to Analytical Instrumentation

4. SPME Commercial Devices and Fibre Coatings

4.1. Introduction

4.2. Description of SPME Fibre Assemblies and Holders

4.3. Description of Fibre Cores, Coatings and the Coating Process

4.4. A Guide for the Selection of the Appropriate SPME Fibre

5. Automated SPME Systems

5.1. Automated Solid-Phase Microextraction–Gas Chromatography

5.2. Automated SPME–LC

5.3. Other Automated Configurations Involving SPME

6. Calibration

6.1. Introduction

6.2. Traditional Calibration Methods for the Quantification of SPME

6.3. Equilibrium Extraction

6.4. Exhaustive Extraction

6.5. Diffusion-Based Calibration

6.6. Calibration of SPME by Liquid Injection

6.7. Summary

7. Solid-Phase Microextraction Method Development

7.1. Introduction

7.2. SPME Method Development – General

7.3. SPME Method Development for GC Applications

7.4. SPME Method Development for HPLC Applications

7.5. Method Validation

7.6. Concluding Remarks

8. SPME and Environmental Analysis

8.1. Introduction

8.2. Fibre SPME

8.3. In-Tube SPME

8.4. TFME

8.5. Applications of SPME in Various Environmental Sample Matrices

8.6. Applications of SPME for Various Analytes in Environmental Samples

8.7. Concluding Remarks

9. Application of Solid-Phase Microextraction in Food and Fragrance Analysis

9.1. Introduction and Method Development Considerations

9.2. Reviews and Case Studies Involving SPME as an Extraction Procedure

9.3. Concluding Remarks

10. Drug Analysis by SPME

10.1. Introduction

10.2. Fundamentals of Extraction

10.3. Fibre Selection: Adsorption Versus Absorption

10.4. Considerations of Drug Properties

10.5. Calibration

10.6. Novel SPME Coatings for LC

10.7. Derivatisation

10.8. Instrumental Configurations

10.9. Applications

10.10. Conclusions

11. Ligand—Receptor Binding and Determination of Free Concentrations

11.1. Introduction

11.2. Analysis of Biological Samples

11.3. Determination of Free Concentrations and Binding Constants

11.4. Calibration of SPME for Bioanalytical Applications

11.5. Conclusions

12. In Vivo Sampling with Solid-Phase Microextraction

12.1. Introduction

12.2. In Vivo Method Development

12.3. In Vivo Applications

12.4. Conclusions

13. Solid-Phase Microextraction Protocols

13.1. Protocol for Automated High-Throughput SPME-LC using the Concept 96 Robotic Sample Preparation Station

13.2. Protocol for Automation of Ligand-Receptor Binding Studies Using Concept 96

13.3. In Vivo SPME Protocol for Direct Monitoring of Circulating Intravenous Blood Concentrations

13.4. Protocol for Setting up Automated SPME-GC Methods


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© Elsevier 2012
1st December 2011
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About the Editor

Janusz Pawliszyn

Janusz Pawliszyn

The primary focus of Professor Pawliszyn's research program is the design of highly automated and integrated instrumentation for the isolation of analytes from complex matrices and the subsequent separation, identification and determination of these species. The primary separation tools used by his group are Gas Chromatography, Liquid Chromatography and Capillary Electrophoresis coupled to variety of detections systems, including range of mass spectrometry techniques. Currently his research is focusing on elimination of organic solvents from the sample preparation step to facilitate on-site monitoring and in-vivo analysis. Several alternative techniques to solvent extraction are investigated including use of coated fibers, packed needles, membranes and supercritical fluids. Dr. Pawliszyn is exploring application of the computational and modeling techniques to enhance performance of sample preparation, chromatographic separations and detection. The major area of his interest involves the development and application of imaging detection techniques for microcolumn chromatography, capillary electrophoresis and micro chip separation devices.

He is an author of over 400 scientific publications and a book on Solid Phase Microextraction. His Hirsch Index (H-index) is 69. He is a Fellow of Royal Society of Canada and Chemical Institute of Canada, editor of Analytica Chimica Acta, Trends in Analytical Chemistry and a member of the Editorial Board of Journal of Separation Science. He initiated a conference, "ExTech", focusing on new advances in sample preparation and disseminates new scientific developments in the area, which meets every year in different part of the world. He received the 1995 McBryde Medal, the 1996 Tswett Medal, the 1996 Hyphenated Techniques in Chromatography Award, the 1996 Caledon Award, the Jubilee Medal 1998 from the Chromatographic Society, U.K., the 2000 Maxxam Award from Canadian Society for Chemistry, the 2000 Varian Lecture Award from Carleton University, the Alumni Achievement Award for 2000 from Southern Illinois University, the Humboldt Research Award for 2001, 2002 COLACRO Medal, 2003 Canada Research Chair, in 2006 he has been elected to the most cited chemists by ISI, in 2008 he received A.A. Benedetti-Pichler Award from Eastern Analytical Symposium, 2008 Andrzej Waksmundzki Medal from Polish Academy of Sciences, 2008 Manning Principal Award, 2010 Torbern Bergman Medal from the Swedish Chemical Society, 2010 Ontario Premier's Innovation Award, 2010 Marcel Golay Award, 2010 ACS Award in Separation Science and Technology and 2011 PittCon Dal Nogare Award. He presently holds the Canada Research Chair and Natural Sciences and Engineering Research Council of Canada Industrial Research Chair in New Analytical Methods and Technologies. He presently holds the University Professor title, the Canada Research Chair and NSERC Industrial Research Chair in New Analytical Methods and Technologies. His Hirsh Index ("H" Index) is 70.

Affiliations and Expertise

University Professor and Canada Research Chair, Department of Chemistry, University of Waterloo, Ontario

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