Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.

Key Features

* Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics


Students and researchers in global academic research in health science institutes and the pharmaceutical industry

Table of Contents

Preface (D. Garfin, S. Ahuja).
Incidents of Travel in IEF and IPGs (P. Giorgio Righetti).
Evolution and Development of IEP (A. Vegvari, F. Kilar).
Theory and Simulation of IEF (T. L. Sounart et al.)
Generation of pH Gradients (T. Berkelman).
Slab Gel IEF (R. Westermeier).
Two-Dimensional Gel Electrophoresis (M.P. Mollay et al.).
Some Practices and Pitfalls of Sample Preparation for IEF in Proeomics (B. Herbert).
Protein Detection and Imaging in IEF Gels (W.P. Patton).
Capillary IEF (T. Wehr).
Free Flow IEF (P.J.A. Weber et al.).
Isoelectric Focusing and Proteomics (S.J. Cordwell, M.Y. White).
Chromatofocusing (D. Anderson).
Alternative Forms of IEF (C.F. Ivory).


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© 2005
Academic Press
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About the editors

David Garfin

Affiliations and Expertise

Garfin Consulting, Kensington, California, USA

Satinder Ahuja

Satinder Ahuja earned his PhD in analytical chemistry from the University of the Sciences, Philadelphia. He worked for Novartis Corp. in various leadership positions for over 25 years while simultaneously teaching and guiding research as an adjunct professor at Pace University for over 10 years. As president of Ahuja Consulting, he advises on quality issues relating to chemicals/pharmaceuticals and water. Dr. Ahuja has published numerous papers and more than eighteen books. Two of his latest books are entitled "Water Reclamation and Sustainability" (Elsevier) and Comprehensive "Water Quality and Purification" (Elsevier). Others include "Monitoring Water Quality: Pollution Assessment, Analysis, and Remediation" (Elsevier, 2013); "Novel Solutions to Water Pollution" (ACS Symposium Volume, 2013); "Handbook of Water Purity and Quality" (Elsevier, 2009); and "Arsenic Contamination of Water: Mechanism, Analysis, and Remediation" (Wiley, 2008). A member of the executive committee of Rivers of the World Foundation (ROW), Dr. Ahuja is an expert on improving water quality and has organized numerous global symposia on this topic (American Chemical Society, UNESCO, etc.) In 2013, he gave a lecture on the nexus of food, energy, and water and chaired a symposium on the subject for the American Chemical Society meeting in New Orleans.

Affiliations and Expertise

Ahuja Consulting, Calabash, NC, USA