Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas

Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas

Molecular Genetics; Lung and Breast Carcinomas

1st Edition - May 11, 2004

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  • Editor: M. Hayat
  • eBook ISBN: 9780080495194
  • Hardcover ISBN: 9780123339416

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Description

The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.

Key Features

  • The only book available that translates molecular genetics into cancer diagnosis
  • Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
  • Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
  • Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
  • Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
  • Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
  • Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References

Readership

Graduate and medical students in cancer research, oncology, pathology, biology, immunology, bioinformatics and endocrinology.

Table of Contents

  • Comparison of Immunohistochemistry, in Situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization
    Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization and Immunohistochemistry
    Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization
    Polymerase Chain Reaction
    DNA Microarrays Technology
    Tissue Microarrays and their Modifications in Highthoroughput Analysis of Clinical Specimens
    Gene Expression Profiling Using Laser Microdissection in Cancer Tissues
    Differential Display of Gene Expression in Human Carcinomas
    Serial Analysis of Gene Expression in Human Diseases
    Lung Carcinoma: An Introduction
    Hitopathological Classification and Phenotype of Lung Tumors
    Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma
    Immunohistochemical Expression of MDM2 in Lung Cancer
    Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma
    Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma
    Immunohistochemistry of Laminin-5 in Lung Carcinoma
    Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma
    Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma
    Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma
    Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma
    In Situ Hybridization and Immunohistochemistry of Telomerase in Lung Carcinoma
    Use of Fluorescence in situ Hybridization in Detection of Lung Cancer Cells
    Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma
    Breast Carcinoma: An Introduction
    Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/KDR in Breast Carcinoma
    HER2/Neu Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ Hybridization
    Her-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast Cancer
    Detection of HER-2 Oncogene with Chromogenic in situ Hybridization in Breast Carcinoma
    Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma Patients
    CD10 Expression in Normal Breast and Breast Cancer Tissue
    Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma
    Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma
    Immunohistochemistry of Adhesion Moleculae Ceacam1 Expression in Breast Carcinoma
    Role of Cadherins in Breast Cancer
    Immunohistochemical Expression of Erythropoitein and Erythropoitin Receptor in Breast Carcinoma
    Loss of BRCA1 Expression in Breast Carcinoma
    Role of Immunohistochemical Expression of BRCA1 in Breast Cancer
    Fluorescence in situ Hybridization of BRCA1 Gene in Breast Carcinoma
    Immunohistochemistry of C-MYC Expression in Breast Carcinoma
    Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma: A Possible Molecular Marker for Diagnosis
    Role of the Epidermal Growth Factor Receptor in Breast Carcinoma
    Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer
    Correlation with Proliferation, Apoptosis, and Clinical Outcome
    Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma
    Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma
    Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma
    Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma
    Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma
    Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence
    Role of ETV6-NTRK3 Gene Fusion in Breast Carcinoma
    Role of CA6 Protein Expression in Breast Carcinoma
    Immunohistochemistry of Effusions
    Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas

Product details

  • No. of pages: 556
  • Language: English
  • Copyright: © Academic Press 2004
  • Published: May 11, 2004
  • Imprint: Academic Press
  • eBook ISBN: 9780080495194
  • Hardcover ISBN: 9780123339416

About the Editor

M. Hayat

M. Hayat
Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

Affiliations and Expertise

Distinguished Professor, Department of Biological Sciences, Kean University, Union, NJ, USA

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