Description

It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories.

Key Features

@introbul:Key Features @bul:* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments

Readership

Working chromatographers and other separation scientists (chemical engineers and analytical chemists) in industry (pharmaceutical companies and biotech firms) and academia.

Table of Contents

Preface. S. Ahuja, Bioseparations: An Overview. D.O. O'Keefe, Analysis of Protein Impurities in Pharmaceuticals Derived from Recombinant DNA. M.T.W. Hearn, Physicochemical Factors in Polypeptide and Protein Purification and Analysis by High Performance Liquid Chromatographic Techniques. S.Ahuja, Capillary Electrophoresis of Compounds of Biological Interest. D.E. Garfin, Isoelectric Focusing. D. Gibson, C.E. Costello, Mass Spectrometry of Biomolecules. T. Gu, Liquid-Liquid Partitioning Methods for Bioseparations. R. Harve, R. Bajpai, Separation of Nucleic Acids and Proteins. A.A. Shukla, S.M. Cramer, Bioseparations by Displacement Chromatography. B. Mattiasson, M.P.Nandakumar, Physicochemical Basis for Expanded-Bed Adsorption for Protein Purification. J. Shiloach, R.M. Kennedy, Expanded-Bed Adsorption Process for Protein Capture. R.R. Deshmukh, T.N. Warner, Adsorptive Membranes for Bioseparations. R.M.Nicoud, Simulated Moving Bed Chromatography for Biomolecules. R.R. Deshmukh, W.E. Leitch II, Y.S. Sanghvi, D.L. Cole, Large-Scale Chromatographic Purification of Oligonucleotides. E. Boschetti, A. Jungbauer, Separation of Antibodies by Liquid Chromatograpy. P. Bowles, Processing Plants and Equipment. R.M. Price, A. Sadana, Engineering Process Control of Biosepartion Processes. A. Ramakrishnan, A. Sadana, Economics of Bioseparation Processes. S. Ahuja, Future Developments. Index.

Details

No. of pages:
722
Language:
English
Copyright:
© 2000
Published:
Imprint:
Academic Press
Print ISBN:
9780120455409
Electronic ISBN:
9780080507798

About the editor

Satinder Ahuja

Satinder Ahuja earned his PhD in analytical chemistry from the University of the Sciences, Philadelphia. He worked for Novartis Corp. in various leadership positions for over 25 years while simultaneously teaching and guiding research as an adjunct professor at Pace University for over 10 years. As president of Ahuja Consulting, he advises on quality issues relating to chemicals/pharmaceuticals and water. Dr. Ahuja has published numerous papers and more than eighteen books. Two of his latest books are entitled "Water Reclamation and Sustainability" (Elsevier) and Comprehensive "Water Quality and Purification" (Elsevier). Others include "Monitoring Water Quality: Pollution Assessment, Analysis, and Remediation" (Elsevier, 2013); "Novel Solutions to Water Pollution" (ACS Symposium Volume, 2013); "Handbook of Water Purity and Quality" (Elsevier, 2009); and "Arsenic Contamination of Water: Mechanism, Analysis, and Remediation" (Wiley, 2008). A member of the executive committee of Rivers of the World Foundation (ROW), Dr. Ahuja is an expert on improving water quality and has organized numerous global symposia on this topic (American Chemical Society, UNESCO, etc.) In 2013, he gave a lecture on the nexus of food, energy, and water and chaired a symposium on the subject for the American Chemical Society meeting in New Orleans.

Affiliations and Expertise

Ahuja Consulting, Calabash, NC, USA