This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.

Key Features

Provides up-to-date methods necessary to study genes in yeast.
Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations.
This volume serves as an essential reference for any beginning or experienced researcher in the field.


Biochemists, geneticists, molecular biologists, cell biologists, and microbiologists

Table of Contents

PART I - Functional Genomics

1.Analysis of Gene Function using DNA Microarrays

Andrew P. Capaldi

2.An introduction to microarray data analysis and visualization

Gregg B. Whitworth

3. Genome-wide approaches to monitor pre-mRNA splicing

Maki Inada and Jeff Pleiss

4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites

Philippe Lefrançois, Wei Zheng and Michael Snyder

5. Genome-wide mapping of nucleosomes in yeast

Oliver J. Rando

6. Genome-wide translational profiling by ribosome footprinting

Nicholas T. Ingolia

PART II - Systematic Genetic Analysis

7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone

8. Making temperature-sensitive mutants

Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter

9. Quantitative Genetic Interaction Mapping using the E-MAP Approach

Sean R. Collins, Assen Roguev and Nevan J. Krogan

10. Exploring gene function and drug action using chemogenomic dosage assays

Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow

PART III - Proteomics

11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry

Tobias C. Walther, Jesper V. Olsen and Matthias Mann

12. High-quality binary interactome mapping

Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun

13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics

Bernd Bo


No. of pages:
© 2010
Academic Press
eBook ISBN:
Print ISBN:

About the serial-volume-editor

About the series-volume-editors

Christine Guthrie

Affiliations and Expertise

University of California, San Francisco, U.S.A.

Gerald Fink

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, U.S.A.


Praise for the first edition:
"...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."--Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY (April 2003)