Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis

Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis

2nd Edition - February 27, 2010

Write a review

  • Editors: Christine Guthrie, Gerald Fink
  • eBook ISBN: 9780123751737
  • Hardcover ISBN: 9780123751720

Purchase options

Purchase options
DRM-free (EPub, PDF, Mobi)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.

Key Features

  • Provides up-to-date methods necessary to study genes in yeast
  • Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations
  • Serves as an essential reference for any beginning or experienced researcher in the field


Biochemists, geneticists, molecular biologists, cell biologists, and microbiologists

Table of Contents

  • PART I - Functional Genomics
    1. Analysis of Gene Function using DNA Microarrays
    Andrew P. Capaldi
    2. An introduction to microarray data analysis and visualization
    Gregg B. Whitworth
    3. Genome-wide approaches to monitor pre-mRNA splicing
    Maki Inada and Jeff Pleiss
    4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites
    Philippe Lefrançois, Wei Zheng and Michael Snyder
    5. Genome-wide mapping of nucleosomes in yeast
    Oliver J. Rando
    6. Genome-wide translational profiling by ribosome footprinting
    Nicholas T. Ingolia

    PART II - Systematic Genetic Analysis
    7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe
    Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone
    8. Making temperature-sensitive mutants
    Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter
    9. Quantitative Genetic Interaction Mapping using the E-MAP Approach
    Sean R. Collins, Assen Roguev and Nevan J. Krogan
    10. Exploring gene function and drug action using chemogenomic dosage assays
    Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow

    PART III - Proteomics
    11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry
    Tobias C. Walther, Jesper V. Olsen and Matthias Mann
    12. High-quality binary interactome mapping
    Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun
    13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics
    Bernd Bodenmiller and Rudi Aebersold
    14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells
    Stephen W. Michnick, Po-Hien Ear, Christian Landry, Mohan K. Malleshaiah and Vincent Messier
    15. Yeast Lipid Analysis and Quantification by Mass Spectrometry
    Xue Li Guan, Isabelle Riezman, Markus R. Wenk and Howard Riezman
    16. Mass Spectrometry-Based Metabolomics of Yeast
    Christopher A. Crutchfield, Wenyun Lu, Eugene Melamud, and Joshua D. Rabinowitz

    PART IV - Systems Analysis
    17. Imaging single mRNA molecules in yeast
    Hyun Youk, Arjun Raj and Alexander van Oudenaarden
    18. Reconstructing gene histories in Ascomycota fungi
    IlanWapinski and Aviv Regev
    19. Experimental evolution in yeast: a practical guide
    Maitreya J. Dunham

    PART V - Synthetic Biology
    20. Enhancing stress resistance and production phenotypes through transcriptome engineering
    Felix H. Lam, Franz S. Hartner, Gerald R. Fink, and Gregory Stephanopoulos

    PART VI - Advances in Cytology/Biochemistry
    21. Visualizing yeast chromosomes and nuclear architecture
    Peter Meister, Lutz R. Gehlen, Elisa Varela, Veronique Kalck and Susan M. Gasser
    22. Quantitative localization of chromosomal loci by immunofluorescence
    Donna Garvey Brickner, William Light and Jason H. Brickner
    23. Spinning Disk Confocal Microscopy of Yeast
    Kurt Thorn
    24. Correlative GFP-immunoelectron microscopy in yeast
    Christopher Buser and Kent McDonald
    25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae
    J Ross Buchan, Tracy Nissan and Roy Parker
    26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization
    Daniel Zenklusen and Robert H Singer
    27. The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD)
    Jeffrey L. Brodsky
    28. A protein transformation protocol for introducing yeast prion particles into yeast
    Motomasa Tanaka
    29. Overexpression and Purification of Integral Membrane Proteins in Yeast
    Franklin A. Hays, Zygy Roe-Zurz, and Robert M. Stroud
    30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast
    Simon Alberti, Randal Halfmann and Susan Lindquist

    PART VII - Other Fungi
    31. Genetics and Molecular Biology in Candida albicans
    Hernday, A.D., Noble, S. M., Mitrovich, Q.M., and Johnson, A.D.
    32. Molecular Genetics of Schizosaccharomyces pombe
    Sarah A. Sabatinos and Susan L. Forsburg
    33. Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans
    Cheryl D. Chun and Hiten D. Madhani
    34. The Fungal Genome Initiative and lessons learned from genome sequencing
    Christina A. Cuomo and Bruce W. Birren
    35. Ultradian metabolic cycles in yeast
    Benjamin Tu

Product details

  • No. of pages: 942
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: February 27, 2010
  • Imprint: Academic Press
  • eBook ISBN: 9780123751737
  • Hardcover ISBN: 9780123751720

About the Series Volume Editors

Christine Guthrie

Affiliations and Expertise

University of California, San Francisco, U.S.A.

Gerald Fink

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, U.S.A.

About the Serial Volume Editor

Jonathan Weissman

Ratings and Reviews

Write a review

There are currently no reviews for "Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis"