Description

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology. The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text: o Lentivirus transgenesis o Germline modification using mouse stem cells o Electroporation o Applications of transposons in mouse genetics o Functional genomics using transposon systems o The use of DNA transposons in detecting cancer genes in mice o Recombination, conditional mutagenesis and induction of tamoxifen o Genetic fate mapping using recombinases o Genetic screens mouse ES cells o Gene trap mutagenesis o Mouse mutagenesis o Self- renewal and pluripotency o Transgenic RNAi applications o Gene knockdowns o Tetracycline-controlled transcription o Gene expression profiling of mouse embryos The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.

Key Features

This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. 

It comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

Readership

Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.

Table of Contents

Contributors Preface Volumes in Series Section I. Transgenesis 1. Lentivirus Transgenesis 1. Introduction 2. Generation of Lentiviral Vectors 3. Generation of Transgenic Animals with Lentiviral Vectors 4. Characterization of Transgenic Animals 5. Summary Acknowledgments References 2. Germline Modification Using Mouse Spermatogonial Stem Cells 1. Introduction 2. Establishing and Maintaining a GS Cell Culture 3. Gene Transduction and Genetic Selection of GS Cells 4. Spermatogonial Transplantation and Offspring Production References 3. Embryonic In Vivo Electroporation in the Mouse 1. Introduction 2. Materials 3. In Utero Electroporation 4. Exo Utero Electroporation 5. Analysis of Electroporated Mice Acknowledgments References Section II. Transposons 4. Current Applications of Transposons in Mouse Genetics 1. Introduction 2. Molecular Characteristics of TEs with Activity in Mice 3. Applications of TEs in Mouse Genetics 4. The Future of TEs in Mouse Genetics References 5. Functional Genomics in the Mouse using the Sleeping Beauty Transposon System 1. Introduction 2. Genome-Wide Germline Mutagenesis with the SB Transgenic Approach 3. Region-Specific Chromosome Engineering with the SB Knock-in Approach 4. Concluding Remarks Acknowledgments References 6. The Use of DNA Transposons for Cancer Gene Discovery in Mice 1. Introduction 2. Choice of Transposon 3. Transposon Design for Cancer Screens 4. Whole-Body (Constitutive) Screens 5. Tissue-Specific Mutagenesis 6. Inducible Transposase Expression 7. Mapping of Transposon Integration Sit

Details

No. of pages:
628
Language:
English
Copyright:
© 2010
Published:
Imprint:
Academic Press
eBook ISBN:
9780123854636
Print ISBN:
9780123848826

About the editors

Paul Wassarman

Affiliations and Expertise

Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA, Tel: +1 212 241 8616, Fax: +1 212 427 7532, Email: P.Wassarman@smtplink.mssm.edu.