Guide to Techniques in Mouse Development, Part B

Contributors Preface Volumes in Series Section I. Transgenesis 1. Lentivirus Transgenesis 1. Introduction 2. Generation of Lentiviral Vectors 3. Generation of Transgenic Animals with Lentiviral Vectors 4. Characterization of Transgenic Animals 5. Summary Acknowledgments References 2. Germline Modification Using Mouse Spermatogonial Stem Cells 1. Introduction 2. Establishing and Maintaining a GS Cell Culture 3. Gene Transduction and Genetic Selection of GS Cells 4. Spermatogonial Transplantation and Offspring Production References 3. Embryonic In Vivo Electroporation in the Mouse 1. Introduction 2. Materials 3. In Utero Electroporation 4. Exo Utero Electroporation 5. Analysis of Electroporated Mice Acknowledgments References Section II. Transposons 4. Current Applications of Transposons in Mouse Genetics 1. Introduction 2. Molecular Characteristics of TEs with Activity in Mice 3. Applications of TEs in Mouse Genetics 4. The Future of TEs in Mouse Genetics References 5. Functional Genomics in the Mouse using the Sleeping Beauty Transposon System 1. Introduction 2. Genome-Wide Germline Mutagenesis with the SB Transgenic Approach 3. Region-Specific Chromosome Engineering with the SB Knock-in Approach 4. Concluding Remarks Acknowledgments References 6. The Use of DNA Transposons for Cancer Gene Discovery in Mice 1. Introduction 2. Choice of Transposon 3. Transposon Design for Cancer Screens 4. Whole-Body (Constitutive) Screens 5. Tissue-Specific Mutagenesis 6. Inducible Transposase Expression 7. Mapping of Transposon Integration Sit