Guide to Techniques in Mouse Development, Part A

Guide to Techniques in Mouse Development, Part A

Mice, Embryos, and Cells

1st Edition - August 24, 2010

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  • Editor: Paul Wassarman
  • Paperback ISBN: 9780123848833

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This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

Key Features

  • First of a 2 part update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
  • Comprehensively covers:
    • new techniques for the cryopreservation of gametes and embryos
    • production of transgenic and null (knockout) animals (use of ES cells)
    • generation of conditional/inducible mutant animals
    • use of gene-trap mutagenesis
    • analysis of allele-specific expression
    • use of new reporter constructs
    • humanizing of transgenic animals
    • transcript profiling of mouse development, imaging of mouse development
    • rederivation of animals and use of mouse genomics


Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology

Table of Contents

  • Section I. General Resources
    1. A survey of internet resources for mouse development
    Thomas L. Saunders

    Section II. Handling Mouse Lines
    2. Transport of mouse lines by shipment of live embryos
    Kevin A. Kelley
    3. Strategies and considerations for distributing and recovering mouse lines
    Yubin Du, Wen Xie, and Chengyu Liu
    4. Archiving and distributing mouse lines by sperm cryopreservation, IVF, and embryo transfer
    Hideko Takahashi and Chengyu Liu

    Section III. Gametes and Embryos
    5. Isolation and manipulation of mouse gametes and embryos
    Eveline S. Litscher and Paul M. Wassarman
    6. Cryopreservation of mouse gametes and embryos
    Carlisle P. Landel
    7. Ovarian follicle culture systems for mammals
    David F. Albertini and Gokhan Akkoyunlu
    8. Production of mouse chimaeras by aggregating pluripotent stem cells with embryos
    Andras Nagy, Kristina Nagy, and Marina Gertsenstein
    9. Production of cloned mice from somatic cells, ES cells, and frozen bodies
    Sayaka Wakayama, Eiji Mizutani, and Teruhiko Wakayama
    10. Nuclear transfer in mouse oocytes and embryos
    Zhiming Han, Yong Cheng, Cheng-Guang Liang, and Keith E. Latham
    11. Culture of whole mouse embryos at early post-implantation to organogenesis stages: developmental staging and methods
    Jaime A. Rivera-Perez, Vanessa Jones, and Patrick P. L. Tam
    12. In utero and ex utero surgery on rodent embryos
    Valerie Ngo-Muller and Ken Mueoka

    Section IV. Fertilization
    13. Enhancement of IVF in the mouse by zona-drilling
    Kevin A. Kelley
    14. ICSI in the mouse
    Paula Stein and Richard M. Schultz

    Section V. ES and iPS Cells
    15. A simple procedure for the efficient derivation of mouse ES cells
    Esther Wong, Kenneth Ban, Rafidah Mutalif, Nancy A. Jenkins, Neal G. Copeland, and Colin L. Stewart
    16. Producing fully ES cell-derived mice from 8-cell stage embryo injections
    Thomas M. DeChiara, William T. Poueymirou, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, and David M. Valenzuela
    17. The loss-of-allele assay for ES cell screening and mouse genotyping
    David Frendewey, Rostislav Chernomorsky, Lakeisha Esau, Jinsop Om, Yingzi Xue, Andrew J. Murphy, George D. Yancopoulos, and David M. Valenzuela
    18. Induced pluripotent stem (iPS) cells
    Holm Zaehres, Jeong Beom Kim, and Hans R. Schöler

    Section VI. Imaging Mouse Development
    19. Imaging mouse embryonic development
    Ryan S. Udan and Mary E. Dickinson
    20. Imaging mouse development with confocal time-lapse microscopy
    Sonja Nowotschin, Anna Ferrer-Vaquer, and Anna-Katerina Hadjantonakis
    21. Ultrasound and magnetic resonance microimaging of mouse development
    Brian J. Nieman and Daniel H. Turnbull

    Section VII. Hematopoiesis
    22. Use of transgenic fluorescent reporter mouse lines to monitor hematopoietic and erythroid development during embryogenesis
    Stuart T. Fraser, Joan Isern, and Margaret H. Baron
    23. Identification and in vivo analysis of murine hematopoietic stem cells
    Serine Avagyan, Yacine M. Amrani, and Hans-Willem Snoeck

Product details

  • No. of pages: 536
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: August 24, 2010
  • Imprint: Academic Press
  • Paperback ISBN: 9780123848833

About the Serial Volume Editor

Paul Wassarman

Affiliations and Expertise

Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA

About the Series Volume Editor

Philippe Soriano

Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

Affiliations and Expertise

Professor of Cell, Developmental and Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

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