Guide to Techniques in Mouse Development, Part A

Guide to Techniques in Mouse Development, Part A

Mice, Embryos, and Cells

1st Edition - August 4, 2010

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  • Editor: Paul Wassarman
  • eBook ISBN: 9780080887784
  • Hardcover ISBN: 9780123747754

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Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

Key Features

  • Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
  • Covers new technologies and methodologies, including:
    • new techniques for the cryopreservation of gametes and embryos
    • production of transgenic and null (knockout) animals (use of ES cells)
    • generation of conditional/inducible mutant animals
    • use of gene-trap mutagenesis
    • analysis of allele-specific expresion
    • use of new reporter constructs
    • humanizing of transgenic animals
    • transcript profiling of mouse development
    • imaging of mouse development
    • rederivation of animals and use of mouse genomics


Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.

Table of Contents

  • Section I. General Resources

    1. A survey of internet resources for mouse development

    Thomas L. Saunders

    Section II. Handling Mouse Lines

    2. Transport of mouse lines by shipment of live embryos

    Kevin A. Kelley

    3. Strategies and considerations for distributing and recovering mouse lines

    Yubin Du, Wen Xie, and Chengyu Liu

    4. Archiving and distributing mouse lines by sperm cryopreservation, IVF, and embryo transfer

    Hideko Takahashi and Chengyu Liu

    Section III. Gametes and Embryos

    5. Isolation and manipulation of mouse gametes and embryos

    Eveline S. Litscher and Paul M. Wassarman

    6. Cryopreservation of mouse gametes and embryos

    Carlisle P. Landel

    7. Ovarian follicle culture systems for mammals

    David F. Albertini and Gokhan Akkoyunlu



    8. Production of mouse chimaeras by aggregating pluripotent stem cells with embryos

    Andras Nagy, Kristina Nagy, and Marina Gertsenstein

    9. Production of cloned mice from somatic cells, ES cells, and frozen bodies

    Sayaka Wakayama, Eiji Mizutani, and Teruhiko Wakayama

    10. Nuclear transfer in mouse oocytes and embryos

    Zhiming Han, Yong Cheng, Cheng-Guang Liang, and Keith E. Latham

    11. Culture of whole mouse embryos at early post-implantation to organogenesis stages: developmental staging and methods

    Jaime A. Rivera-Perez, Vanessa Jones, and Patrick P. L. Tam

    12. In utero and ex utero surgery on rodent embryos

    Valerie Ngo-Muller and Ken Mueoka

    Section IV. Fertilization

    13. Enhancement of IVF in the mouse by zona-drilling

    Kevin A. Kelley

    14. ICSI in the mouse

    Paula Stein and Richard M. Schultz

    Section V. ES and iPS Cells

    15. A simple procedure for the efficient derivation of mouse ES cells

    Esther Wong, Kenneth Ban, Rafidah Mutalif, Nancy A. Jenkins, Neal G. Copeland, and Colin L. Stewart

    16. Producing fully ES cell-derived mice from 8-cell stage embryo injections

    Thomas M. DeChiara, William T. Poueymirou, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, and David M. Valenzuela

    17. The loss-of-allele assay for ES cell screening and mouse genotyping

    David Frendewey, Rostislav Chernomorsky, Lakeisha Esau, Jinsop Om, Yingzi Xue, Andrew J. Murphy, George D. Yancopoulos, and David M. Valenzuela

    18. Induced pluripotent stem (iPS) cells

    Holm Zaehres, Jeong Beom Kim, and Hans R. Schöler


    Section VI. Imaging Mouse Development

    19. Imaging mouse embryonic development

    Ryan S. Udan and Mary E. Dickinson

    20. Imaging mouse development with confocal time-lapse microscopy

    Sonja Nowotschin, Anna Ferrer-Vaquer, and Anna-Katerina Hadjantonakis

    21. Ultrasound and magnetic resonance microimaging of mouse development

    Brian J. Nieman and Daniel H. Turnbull


    Section VII. Hematopoiesis

    22. Use of transgenic fluorescent reporter mouse lines to monitor hematopoietic and erythroid development during embryogenesis

    Stuart T. Fraser, Joan Isern, and Margaret H. Baron

    23. Identification and in vivo analysis of murine hematopoietic stem cells

    Serine Avagyan, Yacine M. Amrani, and Hans-Willem Snoeck

Product details

  • No. of pages: 536
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: August 4, 2010
  • Imprint: Academic Press
  • eBook ISBN: 9780080887784
  • Hardcover ISBN: 9780123747754

About the Serial Editor

Paul Wassarman

Affiliations and Expertise

Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA

About the Serial Volume Editor

Philippe Soriano

Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

Affiliations and Expertise

Professor of Cell, Developmental and Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

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