Guide to Techniques in Mouse Development, Part A - 1st Edition - ISBN: 9780123747754, 9780080887784

Guide to Techniques in Mouse Development, Part A, Volume 476

1st Edition

Mice, Embryos, and Cells

Serial Volume Editors: Philippe Soriano
Serial Editors: Paul Wassarman
eBook ISBN: 9780080887784
Hardcover ISBN: 9780123747754
Imprint: Academic Press
Published Date: 26th August 2010
Page Count: 536
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Table of Contents

Section I. General Resources

1. A survey of internet resources for mouse development

Thomas L. Saunders

Section II. Handling Mouse Lines

2. Transport of mouse lines by shipment of live embryos

Kevin A. Kelley

3. Strategies and considerations for distributing and recovering mouse lines

Yubin Du, Wen Xie, and Chengyu Liu

4. Archiving and distributing mouse lines by sperm cryopreservation, IVF, and embryo transfer

Hideko Takahashi and Chengyu Liu

Section III. Gametes and Embryos

5. Isolation and manipulation of mouse gametes and embryos

Eveline S. Litscher and Paul M. Wassarman

6. Cryopreservation of mouse gametes and embryos

Carlisle P. Landel

7. Ovarian follicle culture systems for mammals

David F. Albertini and Gokhan Akkoyunlu



8. Production of mouse chimaeras by aggregating pluripotent stem cells with embryos

Andras Nagy, Kristina Nagy, and Marina Gertsenstein

9. Production of cloned mice from somatic cells, ES cells, and frozen bodies

Sayaka Wakayama, Eiji Mizutani, and Teruhiko Wakayama

10. Nuclear transfer in mouse oocytes and embryos

Zhiming Han, Yong Cheng, Cheng-Guang Liang, and Keith E. Latham

11. Culture of whole mouse embryos at early post-implantation to organogenesis stages: developmental staging and methods

Jaime A. Rivera-Perez, Vanessa Jones, and Patrick P. L. Tam

12. In utero and ex utero surgery on rodent embryos

Valerie Ngo-Muller and Ken Mueoka

Section IV. Fertilization

13. Enhancement of IVF in the mouse by zona-drilling

Kevin A. Kelley

14. ICSI in the mouse

Paula Stein and Rich


Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.

Key Features

  • Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
  • Covers new technologies and methodologies, including:
    • new techniques for the cryopreservation of gametes and embryos
    • production of transgenic and null (knockout) animals (use of ES cells)
    • generation of conditional/inducible mutant animals
    • use of gene-trap mutagenesis
    • analysis of allele-specific expresion
    • use of new reporter constructs
    • humanizing of transgenic animals
    • transcript profiling of mouse development
    • imaging of mouse development
    • rederivation of animals and use of mouse genomics


Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.


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© Academic Press 2010
Academic Press
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Ratings and Reviews

About the Serial Volume Editors

Philippe Soriano Serial Volume Editor

About the Serial Editors

Paul Wassarman Serial Editor

Affiliations and Expertise

Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA, Tel: +1 212 241 8616, Fax: +1 212 427 7532, Email: