
Guide to Protein Purification
Description
Key Features
- Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology
- Assembles chapters on both common and less-common relevant techniques
- Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Readership
Researchers, professors, and students of biochemistry, pharmacology, genetics, molecular biology and cell biology
Table of Contents
1. Why Purify Enzymes?
2. Strategies and Considerations for Protein Purifications (Linn)
3. Bioinformatics in Planning a Protein Purification (Burgess)
4. Preparing a Protein Purification Summary Table (Burgess)
5. Setting up a Laboratory (Deutscher)
6. Buffers: Principle and Practice (Blanchard)
7. Measurement of Enzyme Activity (Harris)
8. Quantitation of Protein (Bailey and Noble)
9. Maintaining Protein Stability (Deutscher)
10. Strategy for Choosing and Expression System (Brondyk)
11. Bacterial Expression Systems (Collart)
12. Yeast Expression Systems (Cregg)
13. Baculovirus-Insect Cell Expression Systems (Jarvis)
14. Mammalian Expression Systems (Geisse and Knopf)
15. Expression of Tagged Proteins (Melhorta)
16. Re-folding of Solubilized Inclusion Body Proetin (Burgess)
17. Preparation of Biological Extract (Grabski)
Product details
- No. of pages: 912
- Language: English
- Copyright: © Academic Press 2009
- Published: November 19, 2009
- Imprint: Academic Press
- Paperback ISBN: 9780123749789