Guide to Biochemistry

Guide to Biochemistry

1st Edition - March 16, 1989

Write a review

  • Author: James C. Blackstock
  • eBook ISBN: 9781483183671

Purchase options

Purchase options
DRM-free (Mobi, PDF, EPub)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Guide to Biochemistry provides a comprehensive account of the essential aspects of biochemistry. This book discusses a variety of topics, including biological molecules, enzymes, amino acids, nucleic acids, and eukaryotic cellular organizations. Organized into 19 chapters, this book begins with an overview of the construction of macromolecules from building-block molecules. This text then discusses the strengths of some weak acids and bases and explains the interaction of acids and bases involving the transfer of a proton from an acid to a base. Other chapters consider the effectiveness of enzymes, which can be appreciated through the comparison of spontaneous chemical reactions and enzyme-catalyzed reactions. This book discusses as well structure and function of lipids. The final chapter deals with the importance and applications of gene cloning in the fundamental biological research, which lies in the preparation of DNA fragments containing a specific gene. This book is a valuable resource for biochemists and students.

Table of Contents

  • Preface


    1 Biological Molecules

    1.1 Cell Theory

    1.2 The Role of Carbon

    1.3 Stereochemistry

    1.4 The Nature of Macromolecules

    1.5 Hybrid Molecules

    1.6 The Functions of Macromolecules

    1.7 Other Functions of Monomeric Units

    2 The Physical Chemistry of Aqueous Systems

    2.1 Ionization and Macromolecules

    2.2 Hydrogen Bonding in Water

    2.3 Ionization of Water

    2.4 pH Scale

    2.5 Theories of Acids and Bases

    2.6 The Relative Strengths of Acids and Bases

    2.7 The Interaction of Aqueous Solutions of Monoprotic Acids and Bases

    2.8 The Interaction of Polyprotic Weak Acids and Strong Bases

    2.9 Buffer Systems

    2.10 Summary of Useful Equations in pH Calculations

    2.11 Example Calculations

    3 Carbohydrates

    3.1 The Definition of Carbohydrate

    3.2 The Monosaccharides

    3.3 The Ring Structures of Monosaccharides

    3.4 Derivatives of Monosaccharides

    3.5 Oligosaccharides

    3.6 Polysaccharides: Homoglycans

    3.7 Polysaccharides: Heteroglycans

    4 Amino Acids and Proteins

    4.1 The Classification of Amino Acids

    4.2 Ionic Properties of Amino Acids

    4.3 The Major Bonds between Amino Acids in Polypeptide Chains

    4.4 The Primary Structure of Proteins

    4.5 The Secondary Structure of Proteins

    4.6 The Tertiary Structure of Proteins

    4.7 The Quaternary Structure of Proteins

    5 Enzymes

    5.1 Comparison of Chemical and Enzymic Reactions

    5.2 Enzyme Architecture

    5.3 Enzyme Classification

    5.4 Enzyme Catalysis

    6 Enzyme Kinetics

    6.1 Factors Influencing the Rate of an Enzyme-Catalysed Reaction

    6.2 Steady-State Enzyme Kinetics

    6.3 Enzyme Inhibition

    6.4 Allostery

    6.5 Isoenzymes

    7 Nucleic Acids

    7.1 The Structure of Nucleotides

    7.2 Polynucleotide Chains

    7.3 The Structure of DNA

    7.4 The Structure of RNA

    7.5 Some Properties of Nucleic Acids

    8 Lipids

    8.1 The Classes of Lipids

    8.2 Fatty Acids

    8.3 Waxes

    8.4 Acylglycerols

    8.5 Phosphoacylglycerols

    8.6 Plasmalogens

    8.7 Sphingolipids

    8.8 Eicosanoids

    8.9 Terpenes

    8.10 Steroids

    8.11 Circulatory Lipid Complexes

    9 Eukaryotic Cellular Organization

    9.1 Prokaryotic and Eukaryotic Cells

    9.2 The Plasma Membrane: Structure

    9.3 The Plasma Membrane: Transport Mechanisms

    9.4 Nuclear Structures

    9.5 Electron-Transporting Organelles

    9.6 Organelles Associated with Protein Synthesis

    9.7 Lysosomes and Microbodies

    9.8 The Cytoskeleton

    10 Principles of Cellular Metabolism

    10.1 Biological Energetics

    10.2 Equilibrium Thermodynamics

    10.3 Coupled Reactions

    10.4 The Role of ATP

    10.5 Metabolic Pathways

    10.6 Intracellular Control of Metabolism

    10.7 Extracellular Control of Metabolism

    11 Carbohydrate Metabolism

    11.1 The Pathways of Carbohydrate Metabolism

    11.2 The Reactions of Glycolysis

    11.3 NAD+ and Glycolysis

    11.4 The Role of Glycogen

    11.5 Glycogenesis and Glycogenolysis

    11.6 The Role of Gluconeogenesis

    11.7 The Reactions of Gluconeogenesis

    11.8 The Role of the Pentose Phosphate Pathway

    11.9 The Reactions of the Pentose Phosphate Pathway

    11.10 The Energetics of Carbohydrate Metabolism

    11.11 The Regulation of Carbohydrate Metabolism

    12 The Tricarboxylate Cycle

    12.1 Role in Metabolism

    12.2 Entry of Pyruvate into the Mitochondrion

    12.3 The Oxidative Decarboxylation of Pyruvate

    12.4 The Reactions of the Tricarboxylate Cycle

    12.5 The Energetics of the Cycle

    12.6 Anaplerosis

    12.7 The Regulation of the Cycle

    12.8 The Glyoxylate Cycle

    13 Mitochondrial Oxidative Phosphorylation

    13.1 The Role of Oxidative Phosphorylation

    13.2 The Structure of Electron-Transport Assemblies

    13.3 Oxidation-Reduction Reactions

    13.4 The Sequence of Electron-Transport Carriers

    13.5 The Synthesis of ATP

    13.6 Mechanisms of Coupling Electron Transport to ATP Synthesis

    13.7 Inhibitors of Oxidative Phosphorylation

    14 Photosynthesis

    14.1 The Role of Photosynthesis

    14.2 The Thylakoid Photosystems

    14.3 The Light Reactions: Electronic Excitation

    14.4 The Light Reactions: Electron Transport

    14.5 The Dark Reactions: The Calvin Cycle

    14.6 The Dark Reactions: Carbohydrate Synthesis

    15 The Metabolism of Lipids

    15.1 Fatty Acids as Energy Sources

    15.2 The β-Oxidation Pathway

    15.3 Ketone Body Metabolism

    15.4 The Synthesis of Fatty Acids

    15.5 The Synthesis of Triacylglycerols

    15.6 The Synthesis of Eicosanoids

    15.7 The Synthesis of Cholesterol

    15.8 The Metabolism of Steroid Hormones

    16 Nitrogen Metabolism

    16.1 The Biosynthesis of Amino Acids in Plants and Microorganisms

    16.2 The Biosynthesis of Amino Acids in Mammals and Humans

    16.3 The Degradation of Amino Acids

    16.4 The Disposal of Amino Nitrogen in the Animal Kingdom

    16.5 The Urea Cycle

    16.6 The Biosynthesis of Heterocyclic Compounds

    16.7 The Hybridoma Technique

    16.8 The Degradation of Heterocyclic Compounds

    17 Gene Expression

    17.1 The Central Dogma

    17.2 Transcription: Initiation and Elongation

    17.3 Transcription: Termination

    17.4 Translation: The Role of Amino-Acid-TRNA Ligases

    17.5 Translation: The Genetic Code

    17.6 Translation: The Mechanism of Protein Biosynthesis

    17.7 Mutation

    17.8 Control of Gene Expression

    18 The Replication of Deoxyribonucleic Acid

    18.1 Semiconservative Replication

    18.2 An Overview of DNA Replication

    18.3 The Enzymology of DNA Replication in Prokaryotes

    18.4 DNA Repair

    19 Gene Cloning

    19.1 Applications of Gene Cloning

    19.2 Outline of Gene Cloning Methodology

    19.3 Vectors Employed in E. Coli

    19.4 Restriction Endonucleases

    19.5 The Transport of Recombinant DNA Molecules into a Host Bacterium

    19.6 Selection of Recombinant DNA

    19.7 DNA-Sequencing Techniques


Product details

  • No. of pages: 268
  • Language: English
  • Copyright: © Butterworth-Heinemann 1989
  • Published: March 16, 1989
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483183671

About the Author

James C. Blackstock

Ratings and Reviews

Write a review

There are currently no reviews for "Guide to Biochemistry"