Growth, Nutrition, and Metabolism of Cells In Culture V3

Growth, Nutrition, and Metabolism of Cells In Culture V3

1st Edition - January 1, 1977

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  • Editor: George Rothblat
  • eBook ISBN: 9780323145985

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Growth, Nutrition, and Metabolism of Cells in Culture, Volume 3, focuses on a number of specific, timely areas of research that make use of cell and tissue culture. The major theme of this volume is growth and its regulation in animal cells. The book includes studies on the role of growth factors in cell culture systems; the effects of cyclic nucleotides in cell proliferation in culture; metabolic regulation during the cell cycle; and the role of the cell surface in growth and metabolic regulation. There are also separate chapters on aspects of abnormal cell growth and metabolism; DNA repair; genetic analysis using cell fusion techniques; the growth of vascular cells in culture for atherosclerosis research; the culture of haploid vertebrate cells for genetic analysis of cell function; data on haploid cell culture; and the value of using cell cultures to test for the possible toxicity of various pharmacologic agents.

Table of Contents

  • List of Contributors


    Contents of Other Volumes

    I. Cell, Tissue, and Organoid Cultures of Blood Vessels

    I. Introduction

    II. Structural and Functional Heterogeneity of Vasculature

    III. Tissue and Organoid Cultures

    IV. Cell Cultures


    2. Haploid Vertebrate Cell Cultures

    I. Introduction

    II. Culture Methods

    III. Isolation of Variant Cell Lines

    IV. Haploid-Diploid Comparisons as a Test for Mutation

    V. The Nature of Haploidy in Vertebrate Cells

    VI. Summary


    3. Cell Fusion in Genetic Analysis

    I. Introduction

    II. Cell Fusion

    III. Cell Hybridization

    IV. Human Chromosome Mapping

    V. Human Regional Chromosome Assignment

    VI. Studies of Primate Evolution

    VII. Regulation of Differentiated Functions in Somatic Cell Hybrids

    VIII. Mapping of the Integration Sites of Oncogenic Viruses in Transformed Cells

    IX. Genetic Control of Malignancy

    X. Genetics of Cancer in Man


    4. The Structural and Functional Roles of Lipids in the Surface of Animal Cells Grown In Vitro

    I. The Fluid State of Lipid Bilayers

    II. Probes of Membrane Structure

    III. Lipid Alterations in Cultured Cells

    IV. Lipid Structure-Function Relationships


    5. Metabolic Regulation in the Cell Cycle

    I. Introduction

    II. The Methodology of Cell Cycle Analysis (Optimal Conditions, Choice of Cells, and Sampling Intervals)

    III. Temporal Regulation of Gene Expression

    IV. Changing Perspectives of the Cell Cycle


    6. DNA Repair in Cultured Mammalian Cells

    I. Introduction

    II. Systems of DNA Repair in Prokaryotes

    III. Measurement of DNA Repair in Cultured Mammalian Cells

    IV. Concluding Remarks


    7. Control of Animal Cell Proliferation

    I. Introduction

    II. Signals

    III. Transmitters

    IV. Receptors

    V. Machines

    VI. Programs and Clocks


    8. Cyclic Nucleotides

    I. Introduction

    II. Cyclic Nucleotide Formation, Intracellular Interactions, and Degradation

    HI. Role of Cyclic Nucleotides in Cellular Regulation

    IV. Summary


    9. Viral Transformation of Cultured Mammalian Cells as a Multistep Process

    I. Introduction and Nomenclature

    II. Transforming Agents

    III. Initiation of Transformation

    IV. Maintenance of Transformed Phenotype and Reversion

    V. Consequences of Transformation

    VI. Possible Pitfalls

    VII. Conclusion


    10. Chemical Carcinogenesis In Vitro

    I. Introduction

    II. Use of Cell Culture Systems to Study the Biological Effects of Chemical Carcinogens

    III. Use of Cell Culture Systems to Study Mechanisms of Chemical Carcinogenesis

    IV. Perspectives


    11. Toxicity Testing In Vitro

    I. Introduction

    II. Toxicology and In Vitro Studies

    III. Parameters and Methods for the Assessment of Toxicity

    IV. Future Prospects


    Author Index

    Subject Index

Product details

  • No. of pages: 562
  • Language: English
  • Copyright: © Academic Press 1977
  • Published: January 1, 1977
  • Imprint: Academic Press
  • eBook ISBN: 9780323145985

About the Editor

George Rothblat

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