Growth, Nutrition, and Metabolism of Cells In Culture V3

1st Edition - January 1, 1977
Editor: George Rothblat
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 4 5 9 8 - 5

Growth, Nutrition, and Metabolism of Cells in Culture, Volume 3, focuses on a number of specific, timely areas of research that make use of cell and tissue culture. The major theme… Read more

Growth, Nutrition, and Metabolism of Cells In Culture V3

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Growth, Nutrition, and Metabolism of Cells in Culture, Volume 3, focuses on a number of specific, timely areas of research that make use of cell and tissue culture. The major theme of this volume is growth and its regulation in animal cells. The book includes studies on the role of growth factors in cell culture systems; the effects of cyclic nucleotides in cell proliferation in culture; metabolic regulation during the cell cycle; and the role of the cell surface in growth and metabolic regulation. There are also separate chapters on aspects of abnormal cell growth and metabolism; DNA repair; genetic analysis using cell fusion techniques; the growth of vascular cells in culture for atherosclerosis research; the culture of haploid vertebrate cells for genetic analysis of cell function; data on haploid cell culture; and the value of using cell cultures to test for the possible toxicity of various pharmacologic agents.

List of Contributors

Preface

Contents of Other Volumes

I. Cell, Tissue, and Organoid Cultures of Blood Vessels

I. Introduction

II. Structural and Functional Heterogeneity of Vasculature

III. Tissue and Organoid Cultures

IV. Cell Cultures

References

2. Haploid Vertebrate Cell Cultures

I. Introduction

II. Culture Methods

III. Isolation of Variant Cell Lines

IV. Haploid-Diploid Comparisons as a Test for Mutation

V. The Nature of Haploidy in Vertebrate Cells

VI. Summary

References

3. Cell Fusion in Genetic Analysis

I. Introduction

II. Cell Fusion

III. Cell Hybridization

IV. Human Chromosome Mapping

V. Human Regional Chromosome Assignment

VI. Studies of Primate Evolution

VII. Regulation of Differentiated Functions in Somatic Cell Hybrids

VIII. Mapping of the Integration Sites of Oncogenic Viruses in Transformed Cells

IX. Genetic Control of Malignancy

X. Genetics of Cancer in Man

References

4. The Structural and Functional Roles of Lipids in the Surface of Animal Cells Grown In Vitro

I. The Fluid State of Lipid Bilayers

II. Probes of Membrane Structure

III. Lipid Alterations in Cultured Cells

IV. Lipid Structure-Function Relationships

References

5. Metabolic Regulation in the Cell Cycle

I. Introduction

II. The Methodology of Cell Cycle Analysis (Optimal Conditions, Choice of Cells, and Sampling Intervals)

III. Temporal Regulation of Gene Expression

IV. Changing Perspectives of the Cell Cycle

References

6. DNA Repair in Cultured Mammalian Cells

I. Introduction

II. Systems of DNA Repair in Prokaryotes

III. Measurement of DNA Repair in Cultured Mammalian Cells

IV. Concluding Remarks

References

7. Control of Animal Cell Proliferation

I. Introduction

II. Signals

III. Transmitters

IV. Receptors

V. Machines

VI. Programs and Clocks

References

8. Cyclic Nucleotides

I. Introduction

II. Cyclic Nucleotide Formation, Intracellular Interactions, and Degradation

HI. Role of Cyclic Nucleotides in Cellular Regulation

IV. Summary

References

9. Viral Transformation of Cultured Mammalian Cells as a Multistep Process

I. Introduction and Nomenclature

II. Transforming Agents

III. Initiation of Transformation

IV. Maintenance of Transformed Phenotype and Reversion

V. Consequences of Transformation

VI. Possible Pitfalls

VII. Conclusion

References

10. Chemical Carcinogenesis In Vitro

I. Introduction

II. Use of Cell Culture Systems to Study the Biological Effects of Chemical Carcinogens

III. Use of Cell Culture Systems to Study Mechanisms of Chemical Carcinogenesis

IV. Perspectives

References

11. Toxicity Testing In Vitro

I. Introduction

II. Toxicology and In Vitro Studies

III. Parameters and Methods for the Assessment of Toxicity

IV. Future Prospects

References

Author Index

Subject Index