Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume discusses the uptake, synthesis, and degradation of biologically important compounds, particularly the major components usually present in tissue culture medium.
The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on early development of cell culture nutrition; the biological effects of serum; the energy metabolism of malignant cells; the gaseous environment of the mammalian cell in culture; and the uptake and utilization of amino acids by cells in culture. Subsequent chapters cover purine and pyrimidine metabolism; lipids in cell culture; the use of cell cultures for sterol metabolism studies; the genetic expressions of human diploid fibroblast cell cultures; and structural features of mammalian complex carbohydrates.
List of Contributors Preface Contents of Volume II
- General Introduction Text References
- Construction of Tissue Culture Media I. Brief History II. Variability, Purity, and Methods of Sterilization III. Design of Chemically Defined Media IV. Current Media V. Conclusion References
- The Role of Serum in the Control of Multiplication of Avian and Mammalian Cells in Culture I. Introduction II. Biological Effects of Serum III. Isolation from Serum of Fractions Having Biological Effects on Cells in Culture IV. Nonserum Proteins and Other Substances Able to Mimic Some Biological Effects of Serum V. Conclusion References
- Some Aspects of the Energy Metabolism of Mammalian Cells I. Introduction II. Studies of Energy Metabolism at Various Levels of Organization III. A Hypothesis on the Relationship of Increased Glycolysis to Tumorigenicity IV. Summary and Concluding Remarks References
- The Gaseous Environment of the Mammalian Cell in Culture I. Introduction II. Physiological Parameters III. Carbon Dioxide IV. Carbon Monoxide References
- Uptake and Utilization of Amino Acids by Cell Cultures I. Introduction II. Uptake of Amino Acids III. Utilization of Amino Acids IV. Conclusion References
- Purine and Pyrimidine Metabolism of Cells in Culture Glossary I. Introduction II. Purine Metabolism III. Pyrimidine Metabolism IV. Deoxyribonucleotide Metabolism V. Nutritional Requirements VI. Selection Systems VII. Hybridization VIII. Future Uses of Human Cells in Culture with Genetically Determined Alterations in Purine and Pyrimidine Metabolism IX. Summary References
- Fatty Acid, Glyceride, and Phospholipid Metabolism I. Introduction II. Origin of Lipids in Cells in Culture IIL Serum Lipids IV. Cellula
- No. of pages:
- © Academic Press 1972
- 1st January 1972
- Academic Press
- eBook ISBN: