Growth, Nutrition, and Metabolism of Cells In Culture V1

Growth, Nutrition, and Metabolism of Cells In Culture V1

1st Edition - January 1, 1972

Write a review

  • Editor: George Rothblat
  • eBook ISBN: 9780323148511

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume discusses the uptake, synthesis, and degradation of biologically important compounds, particularly the major components usually present in tissue culture medium. The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on early development of cell culture nutrition; the biological effects of serum; the energy metabolism of malignant cells; the gaseous environment of the mammalian cell in culture; and the uptake and utilization of amino acids by cells in culture. Subsequent chapters cover purine and pyrimidine metabolism; lipids in cell culture; the use of cell cultures for sterol metabolism studies; the genetic expressions of human diploid fibroblast cell cultures; and structural features of mammalian complex carbohydrates.

Table of Contents

  • List of Contributors


    Contents of Volume II

    1. General Introduction



    2. Construction of Tissue Culture Media

    I. Brief History

    II. Variability, Purity, and Methods of Sterilization

    III. Design of Chemically Defined Media

    IV. Current Media

    V. Conclusion


    3. The Role of Serum in the Control of Multiplication of Avian and Mammalian Cells in Culture

    I. Introduction

    II. Biological Effects of Serum

    III. Isolation from Serum of Fractions Having Biological Effects on Cells in Culture

    IV. Nonserum Proteins and Other Substances Able to Mimic Some Biological Effects of Serum

    V. Conclusion


    4. Some Aspects of the Energy Metabolism of Mammalian Cells

    I. Introduction

    II. Studies of Energy Metabolism at Various Levels of Organization

    III. A Hypothesis on the Relationship of Increased Glycolysis to Tumorigenicity

    IV. Summary and Concluding Remarks


    5. The Gaseous Environment of the Mammalian Cell in Culture

    I. Introduction

    II. Physiological Parameters

    III. Carbon Dioxide

    IV. Carbon Monoxide


    6. Uptake and Utilization of Amino Acids by Cell Cultures

    I. Introduction

    II. Uptake of Amino Acids

    III. Utilization of Amino Acids

    IV. Conclusion


    7. Purine and Pyrimidine Metabolism of Cells in Culture


    I. Introduction

    II. Purine Metabolism

    III. Pyrimidine Metabolism

    IV. Deoxyribonucleotide Metabolism

    V. Nutritional Requirements

    VI. Selection Systems

    VII. Hybridization

    VIII. Future Uses of Human Cells in Culture with Genetically Determined Alterations in Purine and Pyrimidine Metabolism

    IX. Summary


    8. Fatty Acid, Glyceride, and Phospholipid Metabolism

    I. Introduction

    II. Origin of Lipids in Cells in Culture

    IIL Serum Lipids

    IV. Cellular Uptake of Serum Lipids

    V. Lipid Biosynthesis

    VI. Lipid Utilization

    VII. Summary and Conclusions


    9. Cellular Sterol Metabolism

    I. Introduction

    II. Cellular Sterol Content and Intracellular Location

    III. Sterol Flux

    IV. Sterol Synthesis

    V. Regulation of Sterol Synthesis by Sterol Flux

    VI. Cholesteryl Ester Metabolism

    VII. Summary


    10. Human Diploid Cell Cultures: Their Usefulness in the Study of Genetic Variations in Metabolism

    I. Introduction

    II. Historical Development

    III. Human Diploid Cultures

    IV. Genetic Variations in the Metabolism of Human Diploid Fibroblast Cells

    V. Summary


    11. Complex Carbohydrates of Mammalian Cells in Culture

    I. Introduction

    II. General Structural Features of Mammalian Complex Carbohydrates

    III. Biosynthesis

    IV. Postbiosynthetic Processes

    V. Cellular Physiology and Complex Carbohydrates


    Author Index

    Subject Index

Product details

  • No. of pages: 486
  • Language: English
  • Copyright: © Academic Press 1972
  • Published: January 1, 1972
  • Imprint: Academic Press
  • eBook ISBN: 9780323148511

About the Editor

George Rothblat

Ratings and Reviews

Write a review

There are currently no reviews for "Growth, Nutrition, and Metabolism of Cells In Culture V1"