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Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume discusses the uptake, synthesis, and degradation of biologically important compounds, particularly the major components usually present in tissue culture medium.
The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on early development of cell culture nutrition; the biological effects of serum; the energy metabolism of malignant cells; the gaseous environment of the mammalian cell in culture; and the uptake and utilization of amino acids by cells in culture. Subsequent chapters cover purine and pyrimidine metabolism; lipids in cell culture; the use of cell cultures for sterol metabolism studies; the genetic expressions of human diploid fibroblast cell cultures; and structural features of mammalian complex carbohydrates.
List of Contributors
Contents of Volume II
1. General Introduction
2. Construction of Tissue Culture Media
I. Brief History
II. Variability, Purity, and Methods of Sterilization
III. Design of Chemically Defined Media
IV. Current Media
3. The Role of Serum in the Control of Multiplication of Avian and Mammalian Cells in Culture
II. Biological Effects of Serum
III. Isolation from Serum of Fractions Having Biological Effects on Cells in Culture
IV. Nonserum Proteins and Other Substances Able to Mimic Some Biological Effects of Serum
4. Some Aspects of the Energy Metabolism of Mammalian Cells
II. Studies of Energy Metabolism at Various Levels of Organization
III. A Hypothesis on the Relationship of Increased Glycolysis to Tumorigenicity
IV. Summary and Concluding Remarks
5. The Gaseous Environment of the Mammalian Cell in Culture
II. Physiological Parameters
III. Carbon Dioxide
IV. Carbon Monoxide
6. Uptake and Utilization of Amino Acids by Cell Cultures
II. Uptake of Amino Acids
III. Utilization of Amino Acids
7. Purine and Pyrimidine Metabolism of Cells in Culture
II. Purine Metabolism
III. Pyrimidine Metabolism
IV. Deoxyribonucleotide Metabolism
V. Nutritional Requirements
VI. Selection Systems
VIII. Future Uses of Human Cells in Culture with Genetically Determined Alterations in Purine and Pyrimidine Metabolism
8. Fatty Acid, Glyceride, and Phospholipid Metabolism
II. Origin of Lipids in Cells in Culture
IIL Serum Lipids
IV. Cellular Uptake of Serum Lipids
V. Lipid Biosynthesis
VI. Lipid Utilization
VII. Summary and Conclusions
9. Cellular Sterol Metabolism
II. Cellular Sterol Content and Intracellular Location
III. Sterol Flux
IV. Sterol Synthesis
V. Regulation of Sterol Synthesis by Sterol Flux
VI. Cholesteryl Ester Metabolism
10. Human Diploid Cell Cultures: Their Usefulness in the Study of Genetic Variations in Metabolism
II. Historical Development
III. Human Diploid Cultures
IV. Genetic Variations in the Metabolism of Human Diploid Fibroblast Cells
11. Complex Carbohydrates of Mammalian Cells in Culture
II. General Structural Features of Mammalian Complex Carbohydrates
IV. Postbiosynthetic Processes
V. Cellular Physiology and Complex Carbohydrates
- No. of pages:
- © Academic Press 1972
- 1st January 1972
- Academic Press
- eBook ISBN:
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