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Growth, Nutrition, and Metabolism of Cells In Culture V1 - 1st Edition - ISBN: 9780125983013, 9780323148511

Growth, Nutrition, and Metabolism of Cells In Culture V1

1st Edition

Editor: George Rothblat
eBook ISBN: 9780323148511
Imprint: Academic Press
Published Date: 1st January 1972
Page Count: 486
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Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. The chapters are both detailed and comprehensive enough for the specialist and broad enough to provide a general background for the nonspecialist. The present volume discusses the uptake, synthesis, and degradation of biologically important compounds, particularly the major components usually present in tissue culture medium. The book begins by tracing the history of the development of tissue culture. This is followed by separate chapters on early development of cell culture nutrition; the biological effects of serum; the energy metabolism of malignant cells; the gaseous environment of the mammalian cell in culture; and the uptake and utilization of amino acids by cells in culture. Subsequent chapters cover purine and pyrimidine metabolism; lipids in cell culture; the use of cell cultures for sterol metabolism studies; the genetic expressions of human diploid fibroblast cell cultures; and structural features of mammalian complex carbohydrates.

Table of Contents

List of Contributors


Contents of Volume II

1. General Introduction



2. Construction of Tissue Culture Media

I. Brief History

II. Variability, Purity, and Methods of Sterilization

III. Design of Chemically Defined Media

IV. Current Media

V. Conclusion


3. The Role of Serum in the Control of Multiplication of Avian and Mammalian Cells in Culture

I. Introduction

II. Biological Effects of Serum

III. Isolation from Serum of Fractions Having Biological Effects on Cells in Culture

IV. Nonserum Proteins and Other Substances Able to Mimic Some Biological Effects of Serum

V. Conclusion


4. Some Aspects of the Energy Metabolism of Mammalian Cells

I. Introduction

II. Studies of Energy Metabolism at Various Levels of Organization

III. A Hypothesis on the Relationship of Increased Glycolysis to Tumorigenicity

IV. Summary and Concluding Remarks


5. The Gaseous Environment of the Mammalian Cell in Culture

I. Introduction

II. Physiological Parameters

III. Carbon Dioxide

IV. Carbon Monoxide


6. Uptake and Utilization of Amino Acids by Cell Cultures

I. Introduction

II. Uptake of Amino Acids

III. Utilization of Amino Acids

IV. Conclusion


7. Purine and Pyrimidine Metabolism of Cells in Culture


I. Introduction

II. Purine Metabolism

III. Pyrimidine Metabolism

IV. Deoxyribonucleotide Metabolism

V. Nutritional Requirements

VI. Selection Systems

VII. Hybridization

VIII. Future Uses of Human Cells in Culture with Genetically Determined Alterations in Purine and Pyrimidine Metabolism

IX. Summary


8. Fatty Acid, Glyceride, and Phospholipid Metabolism

I. Introduction

II. Origin of Lipids in Cells in Culture

IIL Serum Lipids

IV. Cellular Uptake of Serum Lipids

V. Lipid Biosynthesis

VI. Lipid Utilization

VII. Summary and Conclusions


9. Cellular Sterol Metabolism

I. Introduction

II. Cellular Sterol Content and Intracellular Location

III. Sterol Flux

IV. Sterol Synthesis

V. Regulation of Sterol Synthesis by Sterol Flux

VI. Cholesteryl Ester Metabolism

VII. Summary


10. Human Diploid Cell Cultures: Their Usefulness in the Study of Genetic Variations in Metabolism

I. Introduction

II. Historical Development

III. Human Diploid Cultures

IV. Genetic Variations in the Metabolism of Human Diploid Fibroblast Cells

V. Summary


11. Complex Carbohydrates of Mammalian Cells in Culture

I. Introduction

II. General Structural Features of Mammalian Complex Carbohydrates

III. Biosynthesis

IV. Postbiosynthetic Processes

V. Cellular Physiology and Complex Carbohydrates


Author Index

Subject Index


No. of pages:
© Academic Press 1972
1st January 1972
Academic Press
eBook ISBN:

About the Editor

George Rothblat

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