Globins and Other Nitric Oxide-Reactive Proteins, Part B

Globins and Other Nitric Oxide-Reactive Proteins, Part B

1st Edition - April 22, 2008

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  • Editor: Robert K. Poole
  • eBook ISBN: 9780080877921
  • Hardcover ISBN: 9780123742780

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: This volume is the second of two planned volumes on the topic of globin and other nitric oxide-reactive proteins.


Researchers in academics and industry studying biochemistry.

Table of Contents

  • Section I. Nitric Oxide-Metabolising and Detoxifying Enzymes

    Chapter 1
    Structural Studies on Flavodiiron Proteins
    João B. Vicente, Maria Arménia Carrondo, Miguel Teixeira and Carlos Frazão

    Chapter 2
    Biochemical, Spectroscopic and Thermodynamic Properties of Flavodiiron Proteins
    João B. Vicente, Marta C. Justino, Vera L. Gonçalves, Lígia M. Saraiva and Miguel Teixeira

    Chapter 3
    Kinetic Characterization of the Escherichia coli Nitric Oxide Reductase Flavorubredoxin
    João B. Vicente, Francesca M. Scandurra, Elena Forte, Maurizio Brunori, Paolo Sarti, Miguel Teixeira and Alessandro Giuffrè

    Chapter 4
    Escherichia coli cytochrome c-nitrite reductase NrfA
    Thomas A. Clarke, Paul C. Mills, Susie R. Poock, Julea N. Butt1, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher Söderberg, Stephen Spiro, Jessica Van Wonderen, David J. Richardson

    Chapter 5
    The respiratory NO reductase (NorBC) from Paracoccus denitrificans
    Sarah J. Field, Faye H. Thorndycroft, Andrey D. Matorin,
    David J. Richardson and Nicholas J. Watmough

    Chapter 6
    Redox-controlled dinitrosyl formation at the diiron-oxo center of NorA
    Rainer Cramm, Katja Strube

    Chapter 7
    Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor
    Li Zhangand Hirofumi Shoun

    Chapter 8
    A Quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain
    Maria G. Mason, Rebecca S. Holladay, Peter Nicholls, Mark Shepherd and Chris E. Cooper

    Section II. Sensor Proteins
    Chapter 9
    Cloning, Expression, and Purification of the N-terminal Heme Binding Domain of the Globin-Coupled Sensors
    Jennifer A. Saito, Tracey Allen K. Freitas, and Maqsudul Alam

    Chapter 10
    Oxygen-Sensing Histidine-Protein Kinases: Assays of Ligand-Binding and Turnover of Response-Regulator Substrates
    Marie-Alda Gilles-Gonzalez, Gonzalo Gonzalez, Eduardo H. S. Sousa, and Jason Tuckerman

    Chapter 11
    Reactions of nitric oxide and oxygen with FNR (regulator of Fumarate and Nitrate Reduction), a global transcriptional regulator, during anaerobic growth of Escherichia coli
    Jason C. Crack, Nick E. Le Brun, Andrew J. Thomson, Jeffrey Green and Adrian J. Jervis

    Chapter 12
    Genome wide identification of binding sites for the nitric oxide sensitive transcriptional regulator NsrR.
    Sam Efromovich, David Grainger, Diane Bodenmiller, and Stephen Spiro.

    Chapter 13
    Methods in Enzymology: Globins and other NO-reactive proteins. Characterization of the NO-reactive transcriptional activator NorR.
    Benoît D’Autréaux, Nick Tucker, Stephen Spiro and Ray Dixon

    Section III. Advanced Spectroscopic Methods

    Chapter 14
    Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni: A Comparative Study with Resonance Raman Spectroscopy
    Changyuan Lu, Tsuyoshi Egawa, Masahiro Mukai, Robert K. Pooleand Syun-Ru Yeh

    Chapter 15
    The power of using continuous-wave and pulsed electron paramagnetic resonance methods for the structure analysis of the ferric forms and NO-ligated ferrous forms of globins.
    Sabine Van Doorslaer and Filip Desmet

    Chapter 16
    Oxygen binding to haem proteins in solution, encapsulated in silica gels and in the crystalline state
    Luca Ronda, Stefano Bruno, Serena Faggiano, Stefano Bettati and Andrea Mozzarelli

    Chapter 17
    Characterization of ligand migration mechanisms inside haemoglobins from the analysis of geminate rebinding kinetics
    Stefania Abbruzzetti, Stefano Bruno, Serena Faggiano, Luca Ronda, Elena Grandi, Andrea Mozzarelli, Cristiano Viappiani

    Chapter 18
    Ligand Dynamics in Heme Proteins Observed by
    Fourier Transform Infrared Spectroscopy at Cryogenic Temperatures
    Karin Nienhaus, G. Ulrich Nienhaus

    Chapter 19
    Time-resolved x-ray crystallography of heme proteins
    Vukica Šrajer and William E. Royer, Jr

    Chapter 20
    Structural Dynamics of Myoglobin
    Maurizio Brunori, Dominque Bourgeois and Beatrice Vallone

    Chapter 21
    Use of the Conjugate Peak Refinement Algorithm for Identification of Ligand Binding Pathways in Globins
    Stephen D. Golden and Kenneth W. Olsen

    Chapter 22
    Finding gas migration pathways in proteins using implicit ligand sampling
    Jordi Cohen, Kenneth W Olsen, and Klaus Schulten

    Chapter 23
    Identification of Ligand Binding Pathways in Truncated Hemoglobins Using Locally Enhanced Sampling Molecular Dynamics
    Stephen D. Golden and Kenneth W. Olsen

Product details

  • No. of pages: 712
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: April 22, 2008
  • Imprint: Academic Press
  • eBook ISBN: 9780080877921
  • Hardcover ISBN: 9780123742780

About the Editor

Robert K. Poole

Robert K. Poole
Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years’ experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

Affiliations and Expertise

West Riding Professor of Microbiology, Department of Molecular Biology and Biotechnology, University of Sheffield, UK

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