Gene therapy
Potential Applications of Nanotechnology
Description
Key Features
- Provides an overview of nanotechnology applications in gene therapy, from preparation of nanoparticles to in vitro and in vivo studies
- Details the tools and techniques available for preparation, characterization and in vitro and in vivo study of nanoparticles
- Details the limitations of nanoparticle-mediated gene therapy and proposes ways in which they may be overcome
Readership
Table of Contents
Dedication
List of figures and tables
Acknowledgments
Foreword
Preface
About the author
Chapter 1: Nanotechnology: an introduction
Abstract:
1.1 Introduction
1.2 Definition of nanotechnology
1.3 Structure of the book
Chapter 2: Methods of nanoparticle preparation
Abstract:
2.1 Introduction
2.2 Preparation of nanoparticles by polymerization of monomers
2.3 Preparation of nanoparticles using preformed polymers
2.4 Methods of controlled release
Chapter 3: Tools and techniques for physico-chemical characterization of nanoparticles
Abstract:
3.1 Introduction
3.2 Physico-chemical characterization
Chapter 4: Characterization of nanoparticles: in vitro and in vivo
Abstract:
4.1 Introduction
4.2 In vitro characterization of nanoparticles
4.3 In vivo characterization
4.4 Conclusions
Chapter 5: Theory and limitations to gene therapy
Abstract:
5.1 Introduction
5.2 Mechanism of gene delivery
5.3 Barriers to gene delivery
5.4 Conclusions
Chapter 6: Targeted gene delivery mediated by nanoparticles
Abstract:
6.1 Introduction
6.2 Approaches for targeted gene delivery
6.3 Conclusions
Chapter 7: Polymeric nanoparticles for gene delivery
Abstract:
7.1 Introduction
7.2 Advantages of nanoparticles
7.3 Limitations of nanoparticles
7.4 Conclusions
Chapter 8: Poly-L-lysine nanoparticles
Abstract:
8.1 Introduction
8.2 In vitro and in vivo applications of poly-L-lysine/DNA nanoparticles
8.3 Polylysine-containing peptides for gene delivery
8.4 Conclusions
Chapter 9: Chitosan nanoparticles
Abstract:
9.1 Introduction
9.2 Factors affecting transfection efficiency of chitosan nanoparticles
9.3 Conclusions
Chapter 10: Polyethylenimine nanoparticles
Abstract:
10.1 Introduction
10.2 Derivatives of PEI for in vitro and in vivo gene delivery
10.3 Degradable PEI for gene delivery
10.4 Conclusions
Chapter 11: Atelocollagen
Abstract:
11.1 Introduction
11.2 Atelocollagen-mediated gene delivery
11.3 Conclusions
Chapter 12: Protamine nanoparticles
Abstract:
12.1 Introduction
12.2 Protamine nanoparticles for gene delivery
12.3 Liposome/protamine/ DNA complexes
12.4 Protamine conjugation to other ligands
12.5 Conclusions
Chapter 13: Dendrimers
Abstract:
13.1 Introduction
13.2 Dendrimers in gene delivery
13.3 Conclusions
Chapter 14: Cyclodextrins and cyclodextrin-containing polymers
Abstract:
14.1 Introduction
14.2 Cyclodextrin-embedded polymers
14.3 Polymers with cyclodextrins as pendant groups
14.4 Cyclodextrins as adjuvants for enhanced gene delivery
14.5 Cyclodextrin-based polyrotaxanes
14.6 Conclusions
Chapter 15: Poly(D,L-lactide-co-glycolide)-based nanoparticles
Abstract:
15.1 Introduction
15.2 PLGA nanoparticles for gene delivery
15.3 Chitosan-modified PLGA nanoparticles
15.4 Polyethylenimine-modified PLGA nanoparticles
15.5 Other modifications to PLGA nanoparticles
15.6 Conclusions
Chapter 16: Metallic and inorganic nanoparticles
Abstract:
16.1 Introduction
16.2 Gold nanoparticles
16.3 Mesoporous silica nanoparticles
16.4 MSN for gene delivery
16.5 Polycation-modified MSN for gene delivery
16.6 Conclusions
Index
Product details
- No. of pages: 380
- Language: English
- Copyright: © Woodhead Publishing 2013
- Published: October 31, 2013
- Imprint: Woodhead Publishing
- Hardcover ISBN: 9781907568404
- eBook ISBN: 9781908818645