Frontiers in Physicochemical Biology

Frontiers in Physicochemical Biology

1st Edition - January 1, 1978

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  • Editor: Bernard Pullman
  • eBook ISBN: 9780323147316

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Frontiers in Physicochemical Biology documents the proceedings of an international symposium held in celebration of the 50th anniversary of the Institut de Biologie Physico-Chimique, (Fondation Edmond de Rothschild) in Paris, May 23-27, 1977. The symposium was organized to cover the main topics of modern day molecular biology and biophysics, which are also the major disciplines researched at the Institute. The contributions made by researchers at the symposium are organized into four parts. Part I contains papers on the physical chemistry of fundamental biomolecules. Part II presents studies on physicochemical aspects of the mechanisms of genetic expression. Part III examines the biochemistry of oxygen and hemoglobin. Part IV deals with the study of organized systems.

Table of Contents

  • List of Contributors


    Part I The Physical Chemistry of Fundamental Biomolecules

    1 Current Progress and Future Prospects of the Research on Nucleic Acids

    I. Introduction

    II. Nuclear Magnetic Resonance Studies

    III. Experimental Evaluation of Electrostatic Interaction and Studies on Nonionic Oligonucleotide Analogues

    IV. Future Prospects


    2 The Structure of Transfer RNA Molecules in Solution

    I. Introduction

    II. Tertiary Structure of tRNA in Solution

    III. Ring-Current Shift Theory Analysis of Low-Field nmr Spectra

    IV. Hydrogen Binding of the Ribose 2'-OH Group in RNA

    V. Summary


    3 The Chemistry of Membrane-Active Peptides and Proteins



    4 Conformational Analysis of Polypeptides: Application to Homologous Proteins

    I. Introduction

    II. Standard Geometry

    III. Computation Procedures

    IV. Approaches to the Multiple-Minimum Problem

    V. Computation of Models for the Three-Dimensional Structures of Three Snake Venom Inhibitors

    VI. Concluding Remarks


    5 Aspects of Biomolecules in Their Surroundings: Hydration and Cation Binding

    I. Introduction

    II. Hydration

    III. Cation Binding

    IV. The Triple System: Substrate-Water-Cation or the Problem of the Through-Water Cation Binding


    Part II Physicochemical Aspects of the Mechanisms of Genetic Expression

    6 Site-Directed Mutagenesis as a Tool in Genetics

    I. Phage Qβ and Its Replication

    II. Site-Directed Mutations in Qβ RNA

    III. Perspectives for Site-Directed Mutagenesis


    7 Gene Control by the λ Phage Repressor

    I. Operator Structure

    II. Promoter Structure

    III. λ Repressor

    IV. Repressor Control of Ν and cro

    V. Self-Regulation of cl

    VI. Translational Control of cI

    VII. Summary


    8 Nuclear Magnetic Resonance Studies of Protein-Nucleotide Interactions

    I. Ternary Enzyme-Metal Nucleotide Complexes

    II. Higher Enzyme-Substrate Complexes

    III. Working Enzymes

    IV. Conclusions


    9 The Role of RNA's in the Structure and Function of Ribosomes

    I. A Rapid Survey of Present Knowledge on the Structure of E. coli Ribosomal RNA's

    II. RNA-RNA Interactions

    III. Interactions between Ribosomal RNA's and Proteins


    10 Mechanism of Initiation of Protein Synthesis

    I. Initiation Sequence: Characterization of Factors

    II. Selection of Initiation Signals

    III. Secondary Structure of mRNA as Negative Control

    IV. Part Played by Ribosomal Proteins and Initiation Factors

    V. Specific Mutants Affecting Initiation

    VI. Conclusion


    Part III Biochemistry of Oxygen and Hemoglobin

    11 Molecular Oxygen and Superoxide Dismutase: Environmental Threat and Biological Defense

    I. Induction of Superoxide Dismutase in Escherichia coli K12

    II. Induction of Superoxide Dismutase in E. coli, at Constant Oxygenation, in Response to Changes in Metabolism

    III. A Convenient New Assay for Superoxide Dismutase


    12 Indoleamine 2,3-Dioxygenase: A Superoxide-Utilizing Enzyme

    I. Introduction

    II. Biological Function

    III. Purification and Molecular Properties

    IV. Role of Superoxide Anion

    V. Experiments with KO2

    VI. Interaction of Enzyme with KO2

    VII. Experiments with 18O2-

    VIII. Interaction of Enzyme with Substrate

    IX. Is O2- a Substrate in Vivo?

    X. Conclusion


    13 Reaction Intermediates of D-Amino Acid Oxidase

    I. Introduction

    II. Occurrence of Two Types of Oxidoreduction Intermediates

    III. Lifetime of the Purple Intermediate

    IV Effect of Hydrogen Bonding on the Formation of the Intermediate

    V. Role of Protein in the Formation of the Intermediates

    VI. Reactivity of the Intermediates with Molecular Oxygen


    14 Biological Aspects of Superoxide Dismutase

    I. Introduction

    II. Production of O2-

    III. Superoxide Dismutases

    IV. Variation in SOD Levels during Development of Ceratitis capitata

    V. Erythrocyte SOD, Catalase, and Glutathione Peroxidase Levels in Different Animals

    VI. Biological Utility of O2-

    VII. Toxicity of Superoxide Radicals

    VIII. Ionizing Irradiations

    IX. Chemiluminescence of O2-

    X. Clinical Aspects of SOD Levels in Humans

    XI. General Conclusion


    15 Recent Developments on the Active-Site Structure and Mechanism of Bovine Copper- and Zinc-Containing Superoxide Dismutase

    I. Chemical Structure of the Metal Binding Sites and General Reaction Mechanism of the Enzyme

    II. Anionic Inhibitors of the Enzyme

    III. Spectral Studies of Co2+-Substituted Superoxide Dismutase

    IV. Catalytic Protonation and Deprotonation of the Bridging Imidazole


    16 Binding of Oxygen to Hemoglobin A in the T-State

    I. Models

    II. The Value of L and Properties of R- and T-State Hemoglobins

    III. Effects of Temperature on the Oxygen-Hemoglobin Reaction

    IV. Effect of pH and Effectors

    V. Conclusions and Problems


    Part IV Study of Organized Systems

    17 The Structure and Assembly of the Membrane of Semliki Forest Virus

    I. Semliki Forest Virus

    II. Composition of SFV

    III. Growth Cycle of SFV

    IV. Structure of the Virus Membrane Glycoproteins

    V. The Final Event in Virus Maturation: The Budding



    18 Oscillations: A Property of Organized Systems

    I. General Mechanistics of Dynamic Organization

    II. Nonequilibrium Dynamics

    III. Experimental Observation of Oscillations

    IV. General Remarks


    19 Cation-Induced Regulatory Mechanism of Enzyme Reactions

    I. Introduction

    II. Catalytic Implications of the Polyelectrolyte Theory: An Oversimplified Treatment

    III. Membrane-Bound Enzymes

    IV. Gene Expression Systems

    V General Conclusion


    20 The Proton Pumps of Photosynthesis

    I. Introduction

    II. Protolytic Reactions in Green Plant Photosynthesis

    III. The Proton Pumps of Bacterial Photosynthesis

    IV. Photochemical Reaction Centers


    21 The Photosynthetic Intramembrane Electric Field

    I. Formation of the Membrane Potential in Algae

    II. Relaxation of the Membrane Potential


    Subject Index

Product details

  • No. of pages: 520
  • Language: English
  • Copyright: © Academic Press 1978
  • Published: January 1, 1978
  • Imprint: Academic Press
  • eBook ISBN: 9780323147316

About the Editor

Bernard Pullman

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