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FRET and FLIM Techniques - 1st Edition - ISBN: 9780080549583, 9780080915128

FRET and FLIM Techniques, Volume 33

1st Edition

Series Volume Editor: Theodorus Gadella
Editor: Theodorus Gadella
Paperback ISBN: 9780080549583
eBook ISBN: 9780080915128
Imprint: Elsevier Science
Published Date: 5th December 2008
Page Count: 560
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Table of Contents

Chapter 1. Förster Resonance Energy transfer –FRET what is it, why do it, and how
it’s done. Robert M. Clegg

Chapter 2.Frequency domain FLIM theory, Instrumentation and data analysis. Peter J.
Verveer and Quentin Hanley.

Chapter 3. Time Domain FLIM; Theory, Instrumentation. Hans C. Gerritsen, A.V.
Agronskaia, A.N. Bader, A. Esposito.

Chapter 4. Multidimensional fluorescence imaging. James James McGinty,
Christopher Dunsby, Egidijus Auksorius, Pieter De Beule, Daniel S. Elson, Neil
Galletly, Oliver Hoffman, Gordon Kennedy, Peter M. P. Lanigan, Ian Munro, Björn
Önfelt, Jose Requejo-Isidro, Klaus Suhling, Clifford B. Talbot, M. John Lever,
Andrew J. deMello, Gordon S. Stamp, Mark A. A. Neil and Paul M. W. French

Chapter 5.Visible fluorescent proteins for FRET. Gert-Jan Kremers and Joachim

Chapter 6. Small Molecule-based FRET probes. Amanda Cobos Correa, Oliver
Wichmann and Carsten Schultz.

Chapter 7. Filter FRET: quantitative imaging of sensitized emission. Kees Jalink and
Jacco van Rheenen.

Chapter 8. Spectral Imaging and its use in the measurement of Förster Resonance
Energy Transfer in living cells. Steven S. Vogel, Paul. S. Blank, Srinagesh V.
Koushik and Christopher Thaler.

Chapter 9. Total Internal Reflection Fluorescence Lifetime Imaging Microscopy.
Theodorus W.J. Gadella Jr.

Chapter 10. FRET and FLIM applications in plants. Riyaz A. Bhat.

Chapter 11. Biomedical FRET-FLIM applications. Phill B. Jones, Brian J. Bacskai,
Bradley T. Hyman.

Chapter 12. Reflections on FRET imaging: formalism, probes and implementation.
Elizabeth A. Jares-Erijman and Thomas M. Jovin.


This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.


Cell biologists, biochemists, inorganic chemists, chemical engineers, material scientists, physicists and electrical engineers.


No. of pages:
© Elsevier Science 2008
5th December 2008
Elsevier Science
Paperback ISBN:
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Ratings and Reviews

About the Series Volume Editor

Theodorus Gadella

Affiliations and Expertise

Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands

About the Editor

Theodorus Gadella

Affiliations and Expertise

Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands