FRET and FLIM Techniques

FRET and FLIM Techniques

1st Edition - December 5, 2008

Write a review

  • Editor: Theodorus Gadella
  • Paperback ISBN: 9780080549583
  • eBook ISBN: 9780080915128

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.


Cell biologists, biochemists, inorganic chemists, chemical engineers, material scientists, physicists and electrical engineers.

Table of Contents

  • Chapter 1. Förster Resonance Energy transfer –FRET what is it, why do it, and how
    it’s done. Robert M. Clegg

    Chapter 2.Frequency domain FLIM theory, Instrumentation and data analysis. Peter J.
    Verveer and Quentin Hanley.

    Chapter 3. Time Domain FLIM; Theory, Instrumentation. Hans C. Gerritsen, A.V.
    Agronskaia, A.N. Bader, A. Esposito.

    Chapter 4. Multidimensional fluorescence imaging. James James McGinty,
    Christopher Dunsby, Egidijus Auksorius, Pieter De Beule, Daniel S. Elson, Neil
    Galletly, Oliver Hoffman, Gordon Kennedy, Peter M. P. Lanigan, Ian Munro, Björn
    Önfelt, Jose Requejo-Isidro, Klaus Suhling, Clifford B. Talbot, M. John Lever,
    Andrew J. deMello, Gordon S. Stamp, Mark A. A. Neil and Paul M. W. French

    Chapter 5.Visible fluorescent proteins for FRET. Gert-Jan Kremers and Joachim

    Chapter 6. Small Molecule-based FRET probes. Amanda Cobos Correa, Oliver
    Wichmann and Carsten Schultz.

    Chapter 7. Filter FRET: quantitative imaging of sensitized emission. Kees Jalink and
    Jacco van Rheenen.

    Chapter 8. Spectral Imaging and its use in the measurement of Förster Resonance
    Energy Transfer in living cells. Steven S. Vogel, Paul. S. Blank, Srinagesh V.
    Koushik and Christopher Thaler.

    Chapter 9. Total Internal Reflection Fluorescence Lifetime Imaging Microscopy.
    Theodorus W.J. Gadella Jr.

    Chapter 10. FRET and FLIM applications in plants. Riyaz A. Bhat.

    Chapter 11. Biomedical FRET-FLIM applications. Phill B. Jones, Brian J. Bacskai,
    Bradley T. Hyman.

    Chapter 12. Reflections on FRET imaging: formalism, probes and implementation.
    Elizabeth A. Jares-Erijman and Thomas M. Jovin.

Product details

  • No. of pages: 560
  • Language: English
  • Copyright: © Elsevier Science 2008
  • Published: December 5, 2008
  • Imprint: Elsevier Science
  • Paperback ISBN: 9780080549583
  • eBook ISBN: 9780080915128

About the Series Volume Editor

Theodorus Gadella

Affiliations and Expertise

Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands

About the Editor

Theodorus Gadella

Affiliations and Expertise

Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands

Ratings and Reviews

Write a review

There are currently no reviews for "FRET and FLIM Techniques"