Introduction. M. Brenowitz, D. Senear, E. Jamison, and D. Dalma-Weiszhausz, Quantitative DNAse I Footprinting. A. Mazumder, Footprinting DNA-Protein and RNA-Ligand Interactions by Chemical Nucleolytic Activity of 1,10 Phenanthroline-Copper. J.S. Bashkin and T.D. Tullius, Hydroxyl Radical Footprinting. J.D. Gralla, M. Hsieh, and C. Wong, Permanganate Probing. M.M. Becker, Photofootprinting DNA in Vitro and in Vivo. J. Cao and A. Revzin,Exonuclease III Digestion. J. Cao and A. Revzin, Interference and Missing Contact Footprinting.
Footprinting of Nucleic Acid-Protein Complexes<$> provides protocols for studying the stoichiometry, binding site size and location, and structural changes in nucleic acids caused by their interaction with proteins. The methods are central to studying key biological processes, such as transcription and translation. The techniques are important to experiments in vivo<$>and in vitro<$>, in eukaryotes and in prokaryotes, at qualitative and quantitative levels,and across many disciplines. This book is a laboratory manual of footprinting techniques for studying nucleic acid-protein interactions. It contains clear and concise descriptions of the most important methodologies, and includes in vivo<$> as well as in vitro<$> applications. It is aimed at bench scientists from graduate students on, and should be of value in industrial labs as well as in academic settings. Use of different footprinting approaches can provide unique insights into DNA-protein systems. The protocols containedin this handbook are written to be"user-friendly,"and thus should be conducive to extending the use of footprinting to new systems. The section on quantitative analysis of DNAse I footprints should prove especially useful for in depth evaluation of cooperative interactions.
(For the End User) Provides clear exposition of footprinting techniques for characterizing DNA-protein interactions Covers both protection methods for identifying sites of protein binding and interference methods for determining points of contact between DNA and protein Includes approaches for both in vitro and in vivo measurements High quality, timely, and of lasting practical value in the laboratory
Researchers, technicians, and students in academic and industrial laboratories working in nucleic acid and protein biochemistry, biotechnology, genetics, molecular, cell, and developmental biology, and other areas of biomedicaland biological research.
- No. of pages:
- © Academic Press 1993
- 20th October 1993
- Academic Press
- eBook ISBN:
Michigan State University, East Lansing, Michigan, USA