COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Footprinting of Nucleic Acid-Protein Complexes - 1st Edition - ISBN: 9780125865005, 9781483295015

Footprinting of Nucleic Acid-Protein Complexes

1st Edition

Series Editor: Arnold Revzin
eBook ISBN: 9781483295015
Imprint: Academic Press
Published Date: 20th October 1993
Page Count: 193
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

Introduction. M. Brenowitz, D. Senear, E. Jamison, and D. Dalma-Weiszhausz, Quantitative DNAse I Footprinting. A. Mazumder, Footprinting DNA-Protein and RNA-Ligand Interactions by Chemical Nucleolytic Activity of 1,10 Phenanthroline-Copper. J.S. Bashkin and T.D. Tullius, Hydroxyl Radical Footprinting. J.D. Gralla, M. Hsieh, and C. Wong, Permanganate Probing. M.M. Becker, Photofootprinting DNA in Vitro and in Vivo. J. Cao and A. Revzin,Exonuclease III Digestion. J. Cao and A. Revzin, Interference and Missing Contact Footprinting.


Footprinting of Nucleic Acid-Protein Complexes<$> provides protocols for studying the stoichiometry, binding site size and location, and structural changes in nucleic acids caused by their interaction with proteins. The methods are central to studying key biological processes, such as transcription and translation. The techniques are important to experiments in vivo<$>and in vitro<$>, in eukaryotes and in prokaryotes, at qualitative and quantitative levels,and across many disciplines.
This book is a laboratory manual of footprinting techniques for studying nucleic acid-protein interactions. It contains clear and concise descriptions of the most important methodologies, and includes in vivo<$> as well as in vitro<$> applications. It is aimed at bench scientists from graduate students on, and should be of value in industrial labs as well as in academic settings. Use of different footprinting approaches can provide unique insights into DNA-protein systems. The protocols containedin this handbook are written to be"user-friendly,"and thus should be conducive to extending the use of footprinting to new systems. The section on quantitative analysis of DNAse I footprints should prove especially useful for in depth evaluation of cooperative interactions.

Key Features

(For the End User)
Provides clear exposition of footprinting techniques for characterizing DNA-protein interactions
Covers both protection methods for identifying sites of protein binding and interference methods for determining points of contact between DNA and protein
Includes approaches for both in vitro and in vivo measurements
High quality, timely, and of lasting practical value in the laboratory


Researchers, technicians, and students in academic and industrial laboratories working in nucleic acid and protein biochemistry, biotechnology, genetics, molecular, cell, and developmental biology, and other areas of biomedicaland biological research.


No. of pages:
© Academic Press 1993
20th October 1993
Academic Press
eBook ISBN:

Ratings and Reviews

About the Series Editor

Arnold Revzin

Affiliations and Expertise

Michigan State University, East Lansing, Michigan, USA