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Fluorescent and Luminescent Probes for Biological Activity - 2nd Edition - ISBN: 9780124478367, 9780080531779

Fluorescent and Luminescent Probes for Biological Activity

2nd Edition

A Practical Guide to Technology for Quantitative Real-Time Analysis

Editor: W. Mason
Hardcover ISBN: 9780124478367
eBook ISBN: 9780080531779
Imprint: Academic Press
Published Date: 16th April 1999
Page Count: 647
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Table of Contents

Part 1: Introduction to Fluorescence Microscopy: J.S. Ploem, Fluorescence Microscopy. Part II: Optical Probes and Their Applications: F.H. Kasten, Introduction to Fluorescent Probes: Properties, History, and Applications. R.P. Haugland and I.D. Johnson, Intracellular Ion Indicators. V.L. Singer and R.P. Haugland, Fluorescent Imaging of Nucleic Acids and Protein in Gels. Part III: Using Optical Probes in Cells - Practicalities, Problems, and Pitfalls: D.N. Bowser, S.H. Cody, P.N. Dubbin, and D.A. Williams, Introducing and Calibrating Fluorescent Probes in Cells and Organelles. F. Bobanovic, Electroporation: A Method for Introduction of Non-permeable Molecular Probes. S.L. Shorte and S. Bolsover, Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging. J. Hoyland, Fluorescent Probes in Practice - Potential Artifacts. Part IV: Optical Probes for Specific Molecules, Organelles, and Cells: A.V. Zelenin, Acridine Orange as a Probe for Molecular and Cell Biology. J.W. Kok and D. Hoekstra, Fluorescent Lipid Analogues: Applications in Cell and Membrane Biology. S.R. Adams, B.J. Bacskai, S.S. Taylor and R.Y. Tsien, Optical Probes for Cyclic AMP. Part V: Technology for Qulaitative and Quantitative Detection of Optical Probes in Living Cells: W.T. Mason, J. Dempster, J. Hoyland, T.J. McCann, B. Somasundaram and W. O'Brien, Quantitative Digital Imaging of Biological Activity in Living Cells with Ion-sensitive Fluorescent Probes. J. Dempster, Fast Photomeric Measurements of Cell Function. L.M. Loew, Potentiometric Membrane Dyes and Imaging Membrane Potential in Single Cells. J.-y. Wu, L.B. Cohen, and C.X. Falk, Fast Multisite Optical Measurement of Membrane Potential, with Two Examples. S. Antic and D. Zecevic, Imagine Membrane Potential Changes in Individual Neurons. Part VI: Using Novel Indications for Genetic, Molecular, and Cellular Function: G.B. Sala-Newby, J.M. Kendall, Helen E. Jones, Kathryn M. Taylor, Michael N. Badminton, David H. Llewellyn and A.K. Campbell, Bioluminescent and Chemiluminescent Indicators for Molecular Signaling and Function in Living Cells. G.A. Rutter, A.A. Culbert and J.M. Tavare, Luminescence Imaging of Gene Expression in Single Living Cells. S.R. Kain, Enhanced Variants of the Green Fluorescent Protein for Greater Sensitivity, Different Colors, and Detection of Apoptosis. R. Haggart, N. Bodsworth, and N. Foote, Rapid Detection of Microorganisms. Part VII: Applications of Confocal Microscopy for Optical Probe Imaging: C.J.R. Sheppard, Confocal Microscopy - Principles, Practice, and Options. K.-P. Yip and I. Kurtz, Dual-excitation Confocal Fluorescence Microscopy. Y.S. Prakash, M.S. Kannan, and G.C. Sieck, High-speed Confocal Imaging in Four Dimensions. G. Cox and C. Sheppard, Multiphoton Fluorescence Microscopy. P. Lipp and M.D. Bootman, High-resolution Confocal Imaging of Elementary Ca2+ Signals in Living Cells. A. Ichihara, T. Tanaami, H. Ishida, and M. Shimizu, Confocal Fluorescent Microscopy Using Nipkow Scanner. S.H. Cody and D.A. Williams, Optimizing Confical Microscopy for Thick Biological Specimens. B.R. Masters and B. Chance, Redox Confocal Imaging: Intrinsic Fluorescent Probes of Cellular Metabolism. Part VIII: Advanced Imaging and Light Detection Approaches for Optical Probe Applications: G.J. Puppels, Confocal Raman Microspectroscopy. C. Rothmann, I. Bar-Am, Z. Dubinsky, S. Katz, and Z. Malik, Spectral Imaging of Autofluorescence Molecules and DNA Probes. B. Masters, P.T.C. So, and E. Gratton, Multiphoton Excitation Microscopy and Spectroscopy of Cells, Tissues, and Human Skin In Vivo. R. Wolthuis, T.C. Bakker Schut, P.J. Caspers, H.P.J. Buschman, T.J. R~mer, H.A. Bruining, and G.J. Puppels, Raman Spectroscopic Methods for In Vitro and In Vivo Tissue Characterization. G.J. Puppels, J.M.C.C. Coremans, and H.A. Bruining, In Vivo Semiquantitative NADH-fluorescence Imaging. T.W.J. Gadella Jr., Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications. G. Skews and T. McCann, Detection of Fluorescently Labelled Proteins following Gel Electrophoresis: A Key Enabling Technology for Proteomics. Part IX: CCD Cameras: Key Enabling Technologies for Optical Probe Imaging: P. Tomkins and A. Lyons, Properties of Low-light-level Intensified Cameras. R. Aikens, Properties of Low-light-level Slow-scan Detectors. C.D. Mackay, High-speed Digital CCD Cameras - Principles and Applications. Part X: Flow Cytomeric Methodologies for Measurement of Optical Probes in Living Cells: E.R. Simons, Flow Cytometry: Use of Multiparameter Kinetics to Evaluate Several Activation Parameters Simultaneously in Individual Living Cells. A.M. Gurney, Photolabile Caged Compounds. K. Hahn, J. Kolega, J. Montibeller, R. DeBasio, P. Post, J. Myers and D.L. Taylor, Fluorescent Analogues: Optical Biosensors of the Chemical and Molecular Dynamics of Macromolecules in Living Cells. Part XI: Applications of Optical Probe Imaging to Biological Problems: M. Fricker, C. Plieth, H. Knight, E. Blancaflor, M.R. Knight, N.S. White, S. Gilroy, Fluorescence and Luminescence Techniques to Probe Ion Activities in Living Plant Cells. G. Shamkar and M.A. Horton, Nuclear Calcium: Concepts and Controversies. A.W. de Feijter, J.E. Trosko, and M.H. Wade, Assessment of Gap Junctional Intercellular Communications in Living Cells Using Fluorescence Techniques. K.E. Sawin, J.A. Theriot and T.J. Mitchison, Photoactivation of Fluorescence as a Probe for Cytoskeletal Dynamics in Mitosis and Cell Motility. K. Florine-Casteel, J.L. Lemasters and B. Herman, Video Imaging of Lipid Order. Index.


The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.

Key Features

TOPICS COVERED: @bul:* Single and multiphoton confocal microscopy

  • Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies
  • Applications of new optical probes for imaging proteins in gels
  • Probes and detection technologies for imaging membrane potential in live cells
  • Use of optical probes to detect microorganisms
  • Raman and confocal raman microspectroscopy
  • Fluorescence lifetime imaging microscopy
  • Digital CCD cameras and their application in biological microscopy


Cell biologists, biochemists, molecular biologists, and physiologists; postgraduates and research workers in both academia and industry and clinical researchers. This book will be invaluable for those wishing to start using optical probes and also for researchers in the field who want to use the most up-to-date techniques.


No. of pages:
© Academic Press 1999
16th April 1999
Academic Press
Hardcover ISBN:
eBook ISBN:


Reviews of the First Edition: "...the long-term applications of the methods described cannot be overestimated... those who have just started optical measurements of intracellular molecules will find [the book] very useful." &#;TRENDS IN NEUROSCIENCES "This book is extremely comprehensive, covering all the major and many of the more esoteric areas of the subject... this book stands alone in its field." &#;TRENDS IN PHARMACOLOGICAL SCIENCES

Ratings and Reviews

About the Editor

W. Mason

Dr. Bill Mason founded Life Science Resources in 1992 and until recently was Head of the Laboratory of Neural and Secretory Signalling at the Babraham Institute in Cambridge. He is recognised internationally as a leader in innovative R&D in real time, low light level imaging. Dr. Mason was awarded his PhD from the Physiological Laboratory in the University of Cambridge. He is also a Member of Trinity College, Cambridge. In addition to his academic and commercial interests, Dr. Mason acts as an expert scientific adviser for strategic and funding advisory bodies in the biomedical sciences including Medical Research Council (UK), NSF (USA), the Welcome Trust, the Nuffield Foundation and the Commission of European Communities. He has played a major role in UKÊscience and technology strategy as a Member of the Working Group on Higher Education and Highly Qualified Manpower for the Advisory Council on Science and Technology (ACOST) at the UK Cabinet Office in H.M. Government, where he co-authored consultative documents relating to government policy on education and training in science and technology.

Affiliations and Expertise

Cambridge, U.K.