Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.

Key Features

    • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
    • Covers fluorescence fluctuation spectroscopy
    • Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells

    Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

    Table of Contents

    Contributors

    Preface

    Methods in Enzymology

    Chapter One. FCS in STED Microscopy

    1 Introduction

    2 STED-FCS of Lipid Membrane Dynamics

    3 Lipid Membrane Dynamics

    4 Conclusions

    References

    Further Reading

    Chapter Two. Analyzing Förster Resonance Energy Transfer with Fluctuation Algorithms

    1 Introduction

    2 FRET and FCS

    3 Filtered FCS

    4 Applications

    5 Discussion

    References

    Chapter Three. Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells

    1 Introduction

    2 Selected FFS Studies

    3 Choice of Fluorophores: General Considerations

    4 Cells: General Considerations

    5 Summary

    References

    Chapter Four. Studying the Protein Corona on Nanoparticles by FCS

    1 Introduction

    2 Sample Preparation

    3 Experimental Procedures

    4 Data Analysis

    5 Protein Corona Formation Measured by FCS

    6 Conclusions

    References

    Chapter Five. Studying Antibody–Antigen Interactions with Fluorescence Fluctuation Spectroscopy

    1 Introduction

    2 Binding Model and Experimental Considerations

    3 Studying Antibodies with FFS

    4 Instrumentation

    References

    Chapter Six. Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling

    1 Introduction

    2 A Fluorescence Fluctuation Toolbox

    3 Experimental Implementation

    4 Applications

    5 Conclusion

    References

    Chapter Seven. Interactions in Gene Expression Networks Studied by Two-Photon Fluorescence Fluctuation Spectroscopy

    1 Introduction

    2 In Vitro Interactions Between Proteins and Nucleic Acids Using Fluctuation Approaches

    3 FFM in Live Bacterial Cells

    4 Conclusions and Perspectives

    References

    Chapter Eight. Studying Ion Exchang

    Details

    No. of pages:
    376
    Language:
    English
    Copyright:
    © 2013
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780124055391
    Electronic ISBN:
    9780124058576