Flow Cytometry, Part B

Flow Cytometry, Part B

2nd Edition - November 9, 1994

Write a review

  • Editors: Zbigniew Darzynkiewicz, J. Paul Robinson, Harry Crissman
  • eBook ISBN: 9780080859408

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


From the Reviews of the First Edition:"This is a good reference manual for multi-user facility faced with a wide variety of biological applications."- CYTOMETRY"Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology."- KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLSFlow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells.

Key Features

* Comprehensive methodological coverage in unique style
* In depth treatment of procedures
* Description of each procedure's:

* Theoretical foundations

* Critical aspects

* Possible pitfalls
* Written by authors with extensive experience who:

* Developed or modified the technique

* Describe their experience with different instruments and applications to different cell systems

* Are the Who's Who in Flow Cytometry
* 10 methods cover assessment of apoptosis and other modes of cell death
* Practical, handbook-style presentation works in lab or classroom
* Printed on acid-free paper
* Color plates

Table of Contents

  • J.R. Selden and F. Dolbeare, A Flow Cytometric Technique for Detection of DNA Repair in Mammalian Cells.
    A. Krishan, Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells.
    D. Hedley and S. Chow, Glutathione and Cellular Resistance to Anti-Cancer Drugs.
    F. Lacombe, Assay of Cell Resistance to Ara-C.
    F. Belloc and F. Durrieu, Detection of mRNA Species by Flow Cytometry.
    H. Fischer, J. Hindkjaer, S. Pedersen, J. Koch, C. Brandt, and S. Kølvraa, Primed in Situ Labeling (PRINS) and Fluorescence in Situ Hybridization (FISH).
    B.D. Li, E.A. Timm, Jr., M.C. Riedy, S.P Harlow, and C.C. Stewart, Molecular Phenotyping by Flow Cytometry.
    S.A. Brockman and R.F. Murphy, Isolation and Analysis of Somatic Cell Mutants with Defects in Endocytic Traffic.
    M. Nüsse, W. Beisker, J. Kramer, B.M. Miller, G.A. Schreiber, S. Viaggi, E.M. Weller, and J.M. Wessels, Measurement of Micronuclei by Flow Cytometry.
    D. Evenson and L. Jost, Sperm Chromatin Structure Assay: DNA Denaturability.
    F. Spyratos and M. Brifford, Fine-Needle Cytopuncture and Flow Cytometry in the Characterization of Human Tumors.
    S. Papa and M. Valentini, Functional NK Assays Using Flow Cytometry.
    T.V. Shankey, Antibodies to Intermediate Filament Proteins as Probes for Multiparameter Flow Cytometry of Human Cancers.
    R.E. Duque, Interactive Data Analysis for Evaluation of B-Cell Neoplasia by Flow Cytometry.
    J.W.M. Visser and P. de Vries, Analysis and Sorting of Hematopoietic Stem Cells from Mouse Bone Marrow.
    B.H. Davis and N.C. Bigelow, Reticulocyte Analysis and Reticulocyte Maturity Index.
    K.A. Ault and J. Mitchell, Analysis of Platelets by Flow Cytometry.
    C.J. Janse and P.H. Van Vianen, Flow Cytometry in Malaria Detection.
    J.J. Fawcett, J.L. Longmire, J.C. Martin, L.L. Deaven, and L.S. Cram, Large-Scale Chromosome Sorting.
    J.F. Leary, Strategies for Rare Cell Detection and Isolation.
    J.V. Giorgi, Cell Sorting of Biohazardous Specimens for Assay of Immune Function.
    J.F. Keij, A.C. Groenewegen, and J.W.M. Visser, High-Speed Photodamage Cell Sorting: An Evaluation of the ZAPPER Prototype.
    A. Radbruch, B. Mechtold, A. Thiel, S. Miltenyi, and E. Pflüger, High-Gradient Magnetic Cell Sorting.
    R.E. Durand, Contributions of Flow Cytometry to Studies with Multicell Spheroids.
    J.P. Robinson, P.K. Narayanan, and W.O. Carter, Functional Measurements Using HL-60 Cells.
    J.V. Giorgi and A. Landay, HIV Infection: Diagnosis and Disease Progression Evaluation.
    B.S. Dien, M.S. Peterson, and F. Srienc, Cell-Cycle Analysis of Saccharomyces cerevisiae.
    H.B. Steen, M.W. Jernaes, K. Skarstad, and E. Boye, Staining and Measurement of DNA in Bacteria.
    G. Vesey, J. Narai, N. Ashbolt, K. Williams, and D. Veal, Detection of Specific Microorganisms in Environmental Samples Using Flow Cytometry.
    C.M. Yentsch and S.A. Pomponi, Strategies for Flow Cytometric Analysis of Marine Microalgae and Sponge Cells.
    D.W. Galbraith, Flow Cytometry and Sorting of Plant Protoplasts and Cells.
    M.A. Sommerfelt and E.J. Sorscher, Use of Fluorescence-Activated Cell Sorting for Rapid Isolation of Insect Cells Harboring Recombinant Baculovirus.
    T.M. McHugh, Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soluble Analytes.
    R.E. Durand, Calibration of Flow Cytometer Detector Systems.
    A. Schwartz and E. Fernández-Repollet, Standardization for Flow Cytometry.
    J.A. Steinkamp, Phase-Sensitive Detection Methods for Resolving Fluorescence Emission Signals and Directly Quantifying Lifetime.
    R.P. Haugland, Spectra of Fluorescent Dyes Used in Flow Cytometry.
    Chapter References.

Product details

  • No. of pages: 697
  • Language: English
  • Copyright: © Academic Press 1994
  • Published: November 9, 1994
  • Imprint: Academic Press
  • eBook ISBN: 9780080859408

About the Serial Volume Editors

Zbigniew Darzynkiewicz

Affiliations and Expertise


J. Paul Robinson

Purdue University, W. Lafayette, IN, USA

Affiliations and Expertise

Director of the Purdue University Cytometry Laboratories and Professor of Basic Medical Science and Biomedical Engineering, Purdue University, IN, USA.

Harry Crissman

Affiliations and Expertise

Cell and Molecular Biology Group, Los Alamos National Laboratory, New Mexico, U.S.A.

About the Serial Editors

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Ratings and Reviews

Write a review

There are currently no reviews for "Flow Cytometry, Part B"