Flow Cytogenetics

Human Chromosome Analysis by Flow Cytometry. Instrumentation for Chromosome Analysis and Sorting. Cell Culture for Chromosome Isolation. Chromosome Isolation Procedures. DNA Stains as Cytochemical Probes for Chromosomes. Methods for Estimating Components of Multipeaked Flow Histograms. Univariate Flow Karyotype Analysis. Bivariate Flow Karyotyping. Relation between Radiation-Induced Flow Karyotype Change

This is the first book to be devoted entirely to the application and development of flow techniques in cytogenetics. It provides comprehensive information on the use of flow cytometry and sorting for chromosome classification and purification. Cytogenetics and molecular biologists will find this book an invaluable reference source.

• Practical details for the preparation and analysis of chromosomes using flow cytometry
• Flow karyotyping for sensitive rapid analysis of chromosome normality and the detection of aberrant chromosomes
• Flow sorting as a source of chromosome-specific DNA for gene mapping and recombinant DNA libraries
• Construction and current status of chromosome-specific recombinant DNA libraries

Molecular biologists, cell biologists, and geneticists