Fe-S Cluster Enzymes Part B

Fe-S Cluster Enzymes Part B

1st Edition - January 20, 2018
This is the Latest Edition
  • Editor: Sheila David
  • Hardcover ISBN: 9780128147177
  • eBook ISBN: 9780128147184

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Description

Methods in Enzymology, Volume 599 is the second of two volumes focused on Fe-S cluster enzymes. Topics of interest in this new release include steps towards understanding mitochondrial Fe/S cluster biogenesis, iron sulfur clusters in zinc finger proteins, electrochemistry of Iron-sulfur enzymes, NRVS for Fe in biology and its experiment and basic interpretation, methods for studying iron regulatory protein 1, an important protein in human iron metabolism, the characterization of glutaredoxin Fe-S cluster binding interactions using circular dichroism spectroscopy, fluorescent reporters to track Fe-S cluster assembly and transfer reactions, methods for studying the Fe-S cluster containing base excision repair glycosylase MUTYH, and more.

Key Features

  • Contain contributions from leading authorities on enzymology
  • Informs and updates on all the latest developments in the field

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Steps Towards Understanding Mitochondrial Fe/S Cluster Biogenesis
    Dennis R. Winge
    2. Iron Sulfur Clusters in Zinc Finger Proteins
    Sarah Michel
    3. Electrochemistry of Iron-sulfur Enzymes
    Fraser Armstrong
    4. NRVS for Fe in Biology: Experiment and Basic Interpretation
    Leland Bruce Gee
    5. Methods for studying Iron Regulatory Protein 1: an important protein in human iron metabolism
    Tracey Rouault
    6. Characterization of glutaredoxin Fe-S cluster binding interactions using circular dichroism spectroscopy
    Caryn E. Outten
    7. Conformationally gated electron transfer in nitrogenase. Isolation, purification and characterization of nitrogenase from Gluconacetobacter diazotrophicus
    F. Akif Tezcan and Cedric P. Owens
    8. Fluorescent reporters to track Fe-S cluster assembly and transfer reactions
    David Barondeau
    9. Methods for studying the Fe-S cluster containing base excision repair glycosylase MUTYH
    Sheila David
    10. Advanced X-ray Spectroscopic Methods for Studying Iron-Sulfur-Containing Proteins and Model Complexes
    Serena DeBeer
    11. Chemical and biochemical reconstitution of iron-sulfur cluster assembly
    Roland Lill
    12. Biochemical analyses of human iron-sulfur protein biogenesis and of related diseases
    Roland Lill
    13. Nbp35-Cfd1 Iron sulfur cluster scaffold: a case study in employing fluorescent nucleotide probes to understand NTPase for metallocofactor biosynthesis
    Deborah Perlstein
    14. Methods for efficient large-scale production, purification, and structure determinations Recombinant Fe-S cluster proteins
    John Tainer

Product details

  • No. of pages: 508
  • Language: English
  • Copyright: © Academic Press 2018
  • Published: January 20, 2018
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128147177
  • eBook ISBN: 9780128147184

About the Serial Volume Editor

Sheila David

Sheila S. David is Professor of Chemistry at the Department of Chemistry at the University of California, Davis.

Affiliations and Expertise

Department of Chemistry, University of California, Davis, USA

Latest reviews

(Total rating for all reviews)

  • Nunziatamaio Sat Nov 24 2018

    Fe-S Cluster Enzymes Part B

    I have enjoyed reading this volume enormously. It is well balanced and there is very little redundancy between the different chapters, which nicely complement one another.