Extracellular Glycolipids of Yeasts - 1st Edition - ISBN: 9780124200692, 9780124200968

Extracellular Glycolipids of Yeasts

1st Edition

Biodiversity, Biochemistry, and Prospects

Authors: Ekaterina Kulakovskaya Tatiana Kulakovskaya
eBook ISBN: 9780124200968
Paperback ISBN: 9780124200692
Imprint: Academic Press
Published Date: 10th January 2014
Page Count: 124
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Description

Extracellular Glycolipids of Yeasts: Biodiversity, Biochemistry, and Prospects provides a comprehensive view of the biochemistry, biological activity, and practical application of extracellular glycolipids of yeast. This book brings much-needed clarity to the complex topic of glycolipids and streamlines the rather confusing terminology used for glycolipids. It also provides a wealth of modern data on their composition, structure and properties, biosynthetic pathways, methods of isolation and identification, antifungal activity, and mechanisms of action.

Studies of extracellular glycolipids of yeast now draw the attention of researchers in life science and biotechnology due to numerous recently revealed biological properties of these compounds. These compounds are scientifically and practically promising in medicine and agriculture due to their biosurfactant and fungicidal properties, as well as a number of other biological activities. Extracellular Glycolipids of Yeasts gives researchers studying biochemistry of microorganisms and related biologically active compounds a much-needed guide to the basic data that will aid in these increasingly generative pursuits.

Key Features

  • Provides a clear overview of the basic data on yeast biosurfactants using a simple survey-style approach
  • Delivers comprehensive view of biochemistry, biological activity, and practical application of yeasts to aid in their scientific and practical use
  • Clarifies and simplifies the complex topic of glycolipids, and its often-confusing terminology

Readership

Researchers working on biochemistry of microorganisms and their biologically active compounds; novel fungicides for medicine and agriculture, including plant protection; and students specializing in biochemistry of microorganisms and biotechnology. 

Table of Contents

Acknowledgments

Introduction

Chapter 1. Structure and Occurrence of Yeast Extracellular Glycolipids

1.1 The Structures of Extracellular Glycolipids of Yeast

1.2 Glycolipid Occurrence in Eumycetes

Chapter 2. Methods for Studying Yeast Extracellular Glycolipids

2.1 Culture Media and Methods for Increasing the Yield of Yeast Extracellular Glycolipids

2.2 Purification Methods

2.3 Thin-Layer Chromatography Systems for Glycolipid Detection

2.4 Chemical Methods

2.5 NMR Spectroscopy and Mass Spectrometry

2.6 Methods for Studying Physicochemical Properties and Antifungal and Membrane-Damaging Activities

2.7 Molecular Biology Methods

Chapter 3. Physicochemical Properties of Yeast Extracellular Glycolipids

3.1 Solubility

3.2 Stability During Storage and thermal Stability

3.3 Molecular Masses

3.4 Surface-Active Properties

3.5 Lactonization and Self-Assembly

3.6 Interaction Between Cellobiose Lipids and Artificial Membranes

Chapter 4. Biological Activities of Extracellular Yeast Glycolipids

4.1 Antifungal Activity of Cellobiose Lipids

4.2 Membrane-Damaging Activity of Cellobiose Lipids

4.3 Biological Activities of MELs and Sophorolipids

4.4 The Biological Activities of Rare Fungal Glycolipids

4.5 The Role of Extracellular Glycolipid for Yeast Producers

Chapter 5. Metabolism of Yeast Extracellular Glycolipids

5.1 Biosynthesis of Extracellular Glycolipids

5.2 Catabolism of Extracellular Glycolipids

Chapter 6. Prospects of Practical Application of Sophorolipids, Cellobiose Lipids, and MELs

6.1 Application as Membranotropic Agents

6.2 Prospects of Application of Yeast Extracellular Glycolipids in Industry, Agriculture, and Medicine

6.3 Commercial Products Based on Yeast Extracellular Glycolipids

Appendix. Selected Techniques of Purification and Assay of Extracellular Yeast Glycolipids

A.1 Methods for Cultivating Producers and Obtaining Glycolipids

A.2 Methods of Antifungal Activity Assay

A.3 Methods of Membrane-Damaging Activity Assay

References

Subject Index

The Index of Generic Names

Details

No. of pages:
124
Language:
English
Copyright:
© Academic Press 2014
Published:
Imprint:
Academic Press
eBook ISBN:
9780124200968
Paperback ISBN:
9780124200692

About the Author

Ekaterina Kulakovskaya

Degrees:

Mr.Sci: July 2005 in Biology (Pushchino State University)

Ph.D.: November 2006 in Biochemistry (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): Antifungal yeast glycolipids. (Supervisors Prof. Kulaev, I.S., and Dr. W.I.Golubev Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)

Positions held:

2005-2008: Junior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

2008-Present: Researcher Skryabin Institute of Biochemistry and Physiology of Microorganisms

Affiliations and Expertise

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

Tatiana Kulakovskaya

Degrees:

Mr.Sci: July 1979 in Biochemistry (Moscow State University, 1979)

Ph.D.: Marh 1985 in Biochemistry (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): ATPase and ion transport in yeast vacuoles. (Supervisor Prof. Kulaev, I.S., Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)

Dr. Sci. in Biochemistry (December, 2001) (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): Exopolyphosphatases of Saccharomyces cerevisiae

Positions held:

1984-1989 Junior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

1989-1992 Researcher Skryabin Institute of Biochemistry and Physiology of Microorganisms

1993-2004 Senior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms

2004-2005 Leading Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms

2005-Present: Head of Laboratory of Regulation of Biochemical Processes Skryabin Institute of Biochemistry and Physiology of Microorganisms

Affiliations and Expertise

Laboratory of Biochemical Processes Regulation Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences