Eukaryotic Transcription Factors

4th Edition

Authors: David Latchman
Hardcover ISBN: 9780124371781
eBook ISBN: 9780080531267
Imprint: Academic Press
Published Date: 21st November 2003
Page Count: 384
108.00 + applicable tax
104.00 + applicable tax
64.99 + applicable tax
80.95 + applicable tax
Compatible Not compatible
VitalSource PC, Mac, iPhone & iPad Amazon Kindle eReader
ePub & PDF Apple & PC desktop. Mobile devices (Apple & Android) Amazon Kindle eReader
Mobi Amazon Kindle eReader Anything else

Institutional Access


Now in two-colour throughout, the fourth edition of Eukaryotic Transcription Factors has been completely rewritten and restructured to take into account the tremendous advances in our understanding of transcription factors and the mechanisms by which they act. Considerable emphasis has been given to the interaction between transcription factors and chromatin structure. Also included is an entirely new section on the mediator complex and expansion of the space devoted to co-activators and co-repressors.

This book is essential reading for all those who wish to understand the impact of molecular biology on medicine, whatever their speciality.

Key Features

  • Major families of eukaryotic transcription factors described
  • Mechanisms of gene activation and repression analysed
  • Structure-function relationships indicated
  • Interaction between transcription factors and chromatin structure described
  • Roles in inducible and cell type-specific gene expression
  • Roles in development, differentiation and human diseases including cancer
  • Methods of study in vitro and in vivo


Postgraduates and researchers, as well as advanced undergraduates, in the fields of molecular biology, biochemistry, molecular genetics, developmental biology and endocrinology.

Table of Contents

List of Tables
DNA sequences, transcription factors and chromatin structure
The importance of transcription
Chromatin structure and its remodelling
Chromatin structure and gene regulation
Chromatin remodelling factors
Histone acetylation
DNA sequence elements
The gene promoter
Sequences involved in the basic process of transcription
Sequences involved in regulated transcription
Sequences which act at a distance
Negatively acting DNA sequences
Interaction between factors bound at various sites
Methods for studying transcription factors
Methods for studying DNA-protein interactions
DNA mobility shift assay
DNaseI footprinting assay
Methylation interference assay
In vivo Footprinting assay
Methods for purifying and/or cloning transcription factors
Protein purification
Gene cloning
Use of cloned genes
Domain mapping of transcription factors
Determining the DNA binding specificity of an uncharacterised factor
Identification of target genes for transcription factors
Transcription factors and constitutive transcription
RNA polymerases
The stable transcriptional complex
RNA polymerase I
RNA polymerase III
RNA polymerase II
Stepwise assembly of the RNA polymerase II basal transcriptional
The RNA polymerase holoenzyme
TBP, The universal transcription


No. of pages:
© Academic Press 2004
Academic Press
eBook ISBN:
Hardcover ISBN:

About the Author

David Latchman

Affiliations and Expertise

Master of Birkbeck, University of London, Professor of Genetics, Birkbeck and University College London, UK