Eukaryotic Transcription Factors

Eukaryotic Transcription Factors

4th Edition - November 21, 2003

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  • Author: David Latchman
  • eBook ISBN: 9780080531267

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Description

Now in two-colour throughout, the fourth edition of Eukaryotic Transcription Factors has been completely rewritten and restructured to take into account the tremendous advances in our understanding of transcription factors and the mechanisms by which they act. Considerable emphasis has been given to the interaction between transcription factors and chromatin structure. Also included is an entirely new section on the mediator complex and expansion of the space devoted to co-activators and co-repressors.This book is essential reading for all those who wish to understand the impact of molecular biology on medicine, whatever their speciality.

Key Features

  • Major families of eukaryotic transcription factors described
  • Mechanisms of gene activation and repression analysed
  • Structure-function relationships indicated
  • Interaction between transcription factors and chromatin structure described
  • Roles in inducible and cell type-specific gene expression
  • Roles in development, differentiation and human diseases including cancer
  • Methods of study in vitro and in vivo

Readership

Postgraduates and researchers, as well as advanced undergraduates, in the fields of molecular biology, biochemistry, molecular genetics, developmental biology and endocrinology

Table of Contents

  • DNA sequences, transcription factors and chromatin structure
    The importance of transcription
    Chromatin structure and its remodelling
    Chromatin structure and gene regulation
    Chromatin remodelling factors
    Histone acetylation
    DNA sequence elements
    The gene promoter
    Sequences involved in the basic process of transcription
    Sequences involved in regulated transcription
    Sequences which act at a distance
    Negatively acting DNA sequences
    Interaction between factors bound at various sites
    Conclusions
    References
    Methods for studying transcription factors
    Introduction
    Methods for studying DNA-protein interactions
    DNA mobility shift assay
    DNaseI footprinting assay
    Methylation interference assay
    In vivo Footprinting assay
    Methods for purifying and/or cloning transcription factors
    Protein purification
    Gene cloning
    Use of cloned genes
    Domain mapping of transcription factors
    Determining the DNA binding specificity of an uncharacterised factor
    Identification of target genes for transcription factors
    Conclusions
    References
    Transcription factors and constitutive transcription
    RNA polymerases
    The stable transcriptional complex
    RNA polymerase I
    RNA polymerase III
    RNA polymerase II
    Stepwise assembly of the RNA polymerase II basal transcriptional
    complex
    The RNA polymerase holoenzyme
    TBP, The universal transcription factor?
    Conclusions
    References
    Families of DNA binding transcription factors
    Introduction
    The homeodomain
    Transcription factors in Drosophila development
    The homeobox
    DNA binding by the helix-turn-helix motif in the homeobox
    Regulation of DNA binding specificity by interaction between different
    homeobox proteins
    Homeodomain transcription factors in other organisms
    POU proteins
    Pax proteins
    The two cysteine two histidine finger
    Transcription factors with the two cysteine two histidine finger
    DNA binding by the two cysteine two histidine finger
    The multi-cysteine finger
    Steroid receptors
    DNA binding by the multi-cysteine zinc finger
    The basic DNA binding domain
    The leucine zipper and the basic DNA binding domain
    The helix-loop-helix motif and the basic DNA binding domain
    Dimerisation of basic DNA binding domain-containing factors
    Other DNA-binding motifs
    Conclusions
    References
    Activation of gene expression by transcription factors
    Activation domains
    Nature of activation domains
    Acidic domains
    Glutamine-rich domains
    Proline-rich domains
    Functional relationship of the different activation domains
    Interaction of activation domains with the basal transcriptional complex
    Activators and the basal transcriptional complex
    Stimulation of factor binding
    Stimulation of factor activity
    Interaction of activation domains with other regulatory proteins
    The mediator complex
    TAFs
    CBP and other co-activators
    A multitude of targets for transcriptional activators
    Other targets for transcriptional activators
    Modulation of chromatin structure
    Stimulation of transcriptional elongation
    Conclusions
    Repression of gene expression by transcription factors
    Repression of transcription
    Indirect repression
    Inhibition of activator binding by masking of its DNA binding site
    Inhibition of activator binding by formation of a non-DNA binding
    complex
    Quenching of an activator
    Degradation of an activator
    Direct repression
    Mechanisms of transcriptional repression
    Direct repression by DNA binding transcription factors
    Direct repression by factors binding to the basal transcriptional complex
    Other targets for transcriptional repressors
    Modulation of chromatin structure
    Inhibition of transcriptional elongation
    Conclusions
    References
    Regulation of transcription factor synthesis
    Transcription factor regulation
    Regulated synthesis of transcription factors
    The MyoD transcription factor
    Homeobox transcription factors
    Mechanisms regulating the synthesis of transcription factors
    Regulation of transcription
    Regulation of RNA splicing
    Regulation of translation
    Conclusions
    Regulation of transcription factor
    Evidence for the regulated activity of transcription factors
    Regulation by protein-ligand binding
    Examples of regulation by ligand binding
    The nuclear receptors
    Regulation by protein-protein interactions
    Inhibition of transcription factor activity by protein-protein interaction
    Activation of transcription factors by protein-protein interaction
    Alteration of transcription factor function by protein-protein interaction
    Regulation by protein modification
    Transcription factor modification
    Phosphorylation
    Acetylation
    Methylation
    Ubiquitination
    Regulation by protein degradation and processing
    Role of regulated activity
    Conclusions
    Transcription factors and human disease
    Diseases caused by transcription factor mutations
    Cancer
    Cellular oncogenes and cancer
    Fos, Jun and AP1
    v-erbA and the thyroid hormone receptor
    The myc oncogene
    Other oncogenic transcription factors
    Anti-oncogenes and cancer
    Nature of anti-oncogenes
    p53
    The Retinoblastoma protein
    Other anti-oncogenic transcription factors.
    Conclusions
    References
    Conclusions and future prospects

Product details

  • No. of pages: 384
  • Language: English
  • Copyright: © Academic Press 2003
  • Published: November 21, 2003
  • Imprint: Academic Press
  • eBook ISBN: 9780080531267

About the Author

David Latchman

Affiliations and Expertise

Master of Birkbeck, University of London, Professor of Genetics, Birkbeck and University College London, UK

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