Description

Now in two-colour throughout, the fourth edition of Eukaryotic Transcription Factors has been completely rewritten and restructured to take into account the tremendous advances in our understanding of transcription factors and the mechanisms by which they act. Considerable emphasis has been given to the interaction between transcription factors and chromatin structure. Also included is an entirely new section on the mediator complex and expansion of the space devoted to co-activators and co-repressors. This book is essential reading for all those who wish to understand the impact of molecular biology on medicine, whatever their speciality.

Key Features

* Major families of eukaryotic transcription factors described * Mechanisms of gene activation and repression analysed * Structure-function relationships indicated * Interaction between transcription factors and chromatin structure described * Roles in inducible and cell type-specific gene expression * Roles in development, differentiation and human diseases including cancer * Methods of study in vitro and in vivo

Readership

Postgraduates and researchers, as well as advanced undergraduates, in the fields of molecular biology, biochemistry, molecular genetics, developmental biology and endocrinology.

Table of Contents

Preface
Acknowledgements
List of Tables
DNA sequences, transcription factors and chromatin structure
The importance of transcription
Chromatin structure and its remodelling
Chromatin structure and gene regulation
Chromatin remodelling factors
Histone acetylation
DNA sequence elements
The gene promoter
Sequences involved in the basic process of transcription
Sequences involved in regulated transcription
Sequences which act at a distance
Negatively acting DNA sequences
Interaction between factors bound at various sites
Conclusions
References
Methods for studying transcription factors
Introduction
Methods for studying DNA-protein interactions
DNA mobility shift assay
DNaseI footprinting assay
Methylation interference assay
In vivo Footprinting assay
Methods for purifying and/or cloning transcription factors
Protein purification
Gene cloning
Use of cloned genes
Domain mapping of transcription factors
Determining the DNA binding specificity of an uncharacterised factor
Identification of target genes for transcription factors
Conclusions
References
Transcription factors and constitutive transcription
RNA polymerases
The stable transcriptional complex
RNA polymerase I
RNA polymerase III
RNA polymerase II
Stepwise assembly of the RNA polymerase II basal transcriptional
complex
The RNA polymerase holoenzyme
TBP, The universal transcription

Details

No. of pages:
384
Language:
English
Copyright:
© 2004
Published:
Imprint:
Academic Press
eBook ISBN:
9780080531267
Print ISBN:
9780124371781

Reviews

Now in two-colour throughout, the fourth edition of Eukaryotic Transcription Factors has been completely rewritten and restructured to take into account the tremendous advances in our understanding of transcription factors and the mechanisms by which they act. Considerable emphasis has been given to the interaction between transcription factors and chromatin structure. Also included is an entirely new section on the mediator complex and expansion of the space devoted to co-activators and co-repressors. This book is essential reading for all those who wish to understand the impact of molecular biology on medicine, whatever their speciality.